ANTIRADICAL ACTIVITY OF ESSENTIAL OILS OF FILIPENDULA ULMARIA (L.) MAXIM, HYPERICUM PERFORA-TUM L. AND PULMONARIA MOLLIS WULFEN EX HORNEM OF THE KRASNOYRSK TERRITORI FLORA

The antiradical properties of essential oils from the inflorescences of Filipendula ulmaria (L). Maxim, herbages Hypericum perforatum L. and Pulmonaria mollis Wulfen ex HORNEM., growing on the territory of the Krasnoyarsk territory were studied. For this purpose, the reaction of essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. Essential oil of the plants under study received comprehensive hydroponically. The component composition of the oils was determined by chromatography-mass spectrometry. The main components of essential oil of F. ulmaria inflorescences are methyl salicylate (28.2%), salicylic aldehyde (2.8 %) and linalool (4.9%), essential oil of H. perforatum – γ - amorphene (30.7%), δ-cadinen (7.1%), (E, E)-β-farnesene (5.5%), caryophyllene (5.0%), ledol (5.0%), essential oil of P. mollis – di-n-butyl phthalate (18.7%), docosan (13.4%), tetracosan (11.6 %). The results of the DPPH test showed that the essential oils of the inflorescences of F. ulmaria and the aboveground part of H. perforatum and P. mollis exhibit antiradical activity (ARA). According to the size of the ARA of essential oils, the studied plants can be arranged in the following row: P. mollis > F. ulmaria > H. perforatum.

COMPLEXES OF METALS WITH HYDRAZONE HYDRAZIDE SALICYLIC ACID

Complexes of Cu (II), Ni (II), and Co (II) with hydrazone derivatives of salicylic acid hydrazide and 3,5-di-tert-butyl salicylic aldehyde (LH) were synthesized and the structure of these compounds studied by means of elemental analysis methods, IR and electronic spectroscopy, magnetochemistry and thermal analysis. It revealed that the complexes are monomeric and have a composition [MLXSoI] where M is a metal ion, L is a ligand, X is an inorganic anion, and Sol is a solvent molecule. LH- in the complexes behaves like a monoanionic tridentate ligand. It found that the resulting complexes exhibit noticeable inhibitory and urease activity.

Synthesis and Structure-Activity Relationship Studies of Hydrazide-Hydrazones as Inhibitors of Laccase from Trametes versicolor

A series of hydrazide-hydrazones 1–3, the imine derivatives of hydrazides and aldehydes bearing benzene rings, were screened as inhibitors of laccase from Trametes versicolor. Laccase is a copper-containing enzyme which inhibition might prevent or reduce the activity of the plant pathogens that produce it in various biochemical processes. The kinetic and molecular modeling studies were performed and for selected compounds, the docking results were discussed. Seven 4-hydroxybenzhydrazide (4-HBAH) derivatives exhibited micromolar activity Ki = 24–674 µM with the predicted and desirable competitive type of inhibition. The structure–activity relationship (SAR) analysis revealed that a slim salicylic aldehyde framework had a pivotal role in stabilization of the molecules near the substrate docking site. Furthermore, the presence of phenyl and bulky tert-butyl substituents in position 3 in salicylic aldehyde fragment favored strong interaction with the substrate-binding pocket in laccase. Both 3- and 4-HBAH derivatives containing larger 3-tert-butyl-5-methyl- or 3,5-di-tert-butyl-2-hydroxy-benzylidene unit, did not bind to the active site of laccase and, interestingly, acted as non-competitive (Ki = 32.0 µM) or uncompetitive (Ki = 17.9 µM) inhibitors, respectively. From the easily available laccase inhibitors only sodium azide, harmful to environment and non-specific, was over 6 times more active than the above compounds.

Salicylic aldehyde-n(4)-(4-ethoxyanilinyl) thiosemicarbazone: optimization of synthesis by surface response and structural characteristics

Salicylic aldehyde-N(4)-(4-ethoxyanilinyl)thiosemicarbazone (H2L) was synthesized by condensation between salicylic aldehyde (SA) and N(4)-(4-ethoxyanilinyl)thiosemicarbazide (TSCZ) in presence of glacial acetic acid as a homogeneous catalyst in ethanol. As a result of analysis by the surface response based on Box – Hunter model with the confidence level of 95%, the expectable yield reached the highest point (around 68%) at 80oC for 10 minutes when approximately 1 mole of SA reacted to equivalently 1 mole of TSCZ. The structure of H2L was solved by IR, UV-Vis, 1H and 13C NMR, HSQC, HMBC and HRMS, which indicated that the thioketone is the predominant form of H2L in the solid phase. The UV-Vis data in variety of pH showed that H2L was assigned to be a diacid with pKa1 = 8.8 and pKa2 = 13.6.

Researches the Process of Formation Salicylaldoxime the Condensation of Salicylaldehyde and Hydroxylamine Sulfate with a Polarographic Control

The use of the polarographic method allows us to trace the condensation of salicylicaldehyde with hydroxylamine sulfate, which results in salicylaldoxime formation. This makes it possible to reduce salicylaldoxime losses occurring as a result of its hydrolysis in the course of salicylamine electrosynthesis, by reduction of salicyloxime forming in the course of salicylicaldehyde and hydroxylamine sulfate condensation. It was found that the rate of salicylic aldehyde and hydroxylamine interaction is high. Salicylicaldehyde completely reacts with hydroxylamine when their molar ratio is equal to 1:5, and salicyloxime is formed with quantitatively yield under these conditions.

Water-stable [Ni(salen)]-type electrode material based on phenylazosubstituted salicylic aldehyde imine ligand

We have synthesized a new water-stable [Ni(salen)]-type electrode material, which was found to be a stacked polymer due to the presence of substituents, preventing oxidative coupling of the phenyl rings.