diterpene cyclase
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Biochemistry ◽  
2020 ◽  
Vol 59 (47) ◽  
pp. 4507-4515
Author(s):  
Yue Zhang ◽  
Lisa M. Prach ◽  
Terrence E. O’Brien ◽  
Frank DiMaio ◽  
Daniil M. Prigozhin ◽  
...  

2020 ◽  
Vol 15 (10) ◽  
pp. 2820-2832 ◽  
Author(s):  
Xiaowen Tang ◽  
Fan Zhang ◽  
Tao Zeng ◽  
Wei Li ◽  
Sheng Yin ◽  
...  
Keyword(s):  

2020 ◽  
Vol 18 (29) ◽  
pp. 5586-5588
Author(s):  
Cody Lemke ◽  
Owen Whitham ◽  
Reuben J. Peters

The class II diterpene cyclase from pleuromutilin biosynthesis, selectively with magnesium, catalyzes ring expansion/contraction between its initial mutildienyl diphosphate product and halimadienyl diphosphate, providing novel catalytic insight.


2018 ◽  
Vol 20 (4) ◽  
pp. 1200-1202 ◽  
Author(s):  
Meimei Xu ◽  
Meirong Jia ◽  
Young J. Hong ◽  
Xihou Yin ◽  
Dean J. Tantillo ◽  
...  
Keyword(s):  
Class Ii ◽  

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Kazunori Okada ◽  
Hiroshi Kawaide ◽  
Koji Miyamoto ◽  
Sho Miyazaki ◽  
Ryosuke Kainuma ◽  
...  

2014 ◽  
Vol 70 (6) ◽  
pp. 1528-1537 ◽  
Author(s):  
Ronja Janke ◽  
Christian Görner ◽  
Max Hirte ◽  
Thomas Brück ◽  
Bernhard Loll

Sesquiterpenes and diterpenes are a diverse class of secondary metabolites that are predominantly derived from plants and some prokaryotes. The properties of these natural products encompass antitumor, antibiotic and even insecticidal activities. Therefore, they are interesting commercial targets for the chemical and pharmaceutical industries. Owing to their structural complexity, these compounds are more efficiently accessed by metabolic engineering of microbial systems than by chemical synthesis. This work presents the first crystal structure of a bacterial diterpene cyclase, CotB2 from the soil bacteriumStreptomyces melanosporofaciens, at 1.64 Å resolution. CotB2 is a diterpene cyclase that catalyzes the cyclization of the linear geranylgeranyl diphosphate to the tricyclic cyclooctat-9-en-7-ol. The subsequent oxidation of cyclooctat-9-en-7-ol by two cytochrome P450 monooxygenases leads to bioactive cyclooctatin. Plasticity residues that decorate the active site of CotB2 have been mutated, resulting in alternative monocyclic, dicyclic and tricyclic compounds that show bioactivity. These new compounds shed new light on diterpene cyclase reaction mechanisms. Furthermore, the product of mutant CotB2W288Gproduced the new antibiotic compound (1R,3E,7E,11S,12S)-3,7,18-dolabellatriene, which acts specifically against multidrug-resistantStaphylococcus aureus. This opens a sustainable route for the industrial-scale production of this bioactive compound.


2011 ◽  
Vol 286 (50) ◽  
pp. 42840-42847 ◽  
Author(s):  
Yoshinori Sugai ◽  
Yohei Ueno ◽  
Ken-ichiro Hayashi ◽  
Shingo Oogami ◽  
Tomonobu Toyomasu ◽  
...  

2011 ◽  
Vol 7 (7) ◽  
pp. 431-433 ◽  
Author(s):  
Mustafa Köksal ◽  
Huayou Hu ◽  
Robert M Coates ◽  
Reuben J Peters ◽  
David W Christianson

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