myelin imaging
Recently Published Documents


TOTAL DOCUMENTS

34
(FIVE YEARS 5)

H-INDEX

9
(FIVE YEARS 0)

2021 ◽  
Vol 118 (45) ◽  
pp. e2106143118
Author(s):  
Ming-Yu Wu ◽  
Alex Y. H. Wong ◽  
Jong-Kai Leung ◽  
Chuen Kam ◽  
Kenneth Lap-Kei Wu ◽  
...  

Myelin, the structure that surrounds and insulates neuronal axons, is an important component of the central nervous system. The visualization of the myelinated fibers in brain tissues can largely facilitate the diagnosis of myelin-related diseases and understand how the brain functions. However, the most widely used fluorescent probes for myelin visualization, such as Vybrant DiD and FluoroMyelin, have strong background staining, low-staining contrast, and low brightness. These drawbacks may originate from their self-quenching properties and greatly limit their applications in three-dimensional (3D) imaging and myelin tracing. Chemical probes for the fluorescence imaging of myelin in 3D, especially in optically cleared tissue, are highly desirable but rarely reported. We herein developed a near-infrared aggregation-induced emission (AIE)-active probe, PM-ML, for high-performance myelin imaging. PM-ML is plasma membrane targeting with good photostability. It could specifically label myelinated fibers in teased sciatic nerves and mouse brain tissues with a high–signal-to-background ratio. PM-ML could be used for 3D visualization of myelin sheaths, myelinated fibers, and fascicles with high-penetration depth. The staining is compatible with different brain tissue–clearing methods, such as ClearT and ClearT2. The utility of PM-ML staining in demyelinating disease studies was demonstrated using the mouse model of multiple sclerosis. Together, this work provides an important tool for high-quality myelin visualization across scales, which may greatly contribute to the study of myelin-related diseases.


Author(s):  
KR Campanholo ◽  
MS Pitombeira ◽  
CM Rimkus ◽  
MF Mendes ◽  
SL Apóstolos-Pereira ◽  
...  

2021 ◽  
Vol 17 ◽  
Author(s):  
Vikas Bhatia ◽  
Raghav Seth ◽  
Arushi Gahlot Saini ◽  
Paramjeet Singh

: This article's primary goal is to provide an image-based review to paediatricians to gain insight into the typical appearance of myelin evolution. We briefly discuss the structure and development of myelination, the role of qualitative and quantitative MRI in myelin imaging, and provide an image-based review as a quick reference for understanding the pattern of myelination on MR imaging.


2021 ◽  
pp. 0271678X2110208
Author(s):  
Marina Yu Khodanovich ◽  
Ilya L Gubskiy ◽  
Marina S Kudabaeva ◽  
Darya D Namestnikova ◽  
Alena A Kisel ◽  
...  

Remyelination is a key process enabling post-stroke brain tissue recovery and plasticity. This study aimed to explore the feasibility of demyelination and remyelination monitoring in experimental stroke from the acute to chronic stage using an emerging myelin imaging biomarker, macromolecular proton fraction (MPF). After stroke induction by transient middle cerebral artery occlusion, rats underwent repeated MRI examinations during 85 days after surgery with histological endpoints for the animal subgroups on the 7th, 21st, 56th, and 85th days. MPF maps revealed two sub-regions within the infarct characterized by distinct temporal profiles exhibiting either a persistent decrease by 30%–40% or a transient decrease followed by return to nearly normal values after one month of observation. Myelin histology confirmed that these sub-regions had nearly similar extent of demyelination in the sub-acute phase and then demonstrated either chronic demyelination or remyelination. The remyelination zones also exhibited active axonal regrowth, reconstitution of compact fiber bundles, and proliferation of neuronal and oligodendroglial precursors. The demyelination zones showed more extensive astrogliosis from the 21st day endpoint. Both sub-regions had substantially depleted neuronal population over all endpoints. These results histologically validate MPF mapping as a novel approach for quantitative assessment of myelin damage and repair in ischemic stroke.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Adam V. Dvorak ◽  
Emil Ljungberg ◽  
Irene M. Vavasour ◽  
Lisa Eunyoung Lee ◽  
Shawna Abel ◽  
...  

AbstractThe traditional approach for measuring myelin-associated water with quantitative magnetic resonance imaging (MRI) uses multi-echo T2 relaxation data to calculate the myelin water fraction (MWF). A fundamentally different approach, abbreviated “mcDESPOT”, uses a more efficient steady-state acquisition to generate an equivalent metric (fM). Although previous studies have demonstrated inherent instability and bias in the complex mcDESPOT analysis procedure, fM has often been used as a surrogate for MWF. We produced and compared multivariate atlases of MWF and fM in healthy human brain and cervical spinal cord (available online) and compared their ability to detect multiple sclerosis pathology. A significant bias was found in all regions (p < 10–5), albeit reversed for spinal cord (fM-MWF =  − 3.4%) compared to brain (+ 6.2%). MWF and fM followed an approximately linear relationship for regions with MWF <  ~ 10%. For MWF >  ~ 10%, the relationship broke down and fM no longer increased in tandem with MWF. For multiple sclerosis patients, MWF and fM Z score maps showed overlapping areas of low Z score and similar trends between patients and brain regions, although those of fM generally had greater spatial extent and magnitude of severity. These results will guide future choice of myelin-sensitive quantitative MRI and improve interpretation of studies using either myelin imaging approach.


2020 ◽  
Author(s):  
Timothy W. Chapman ◽  
Genaro E. Olveda ◽  
Elizabeth Pereira ◽  
Robert A. Hill

ABSTRACTMyelin is critical for neural circuit function and its destruction is widespread in neurodegenerative disease and aging. In these conditions, homeostatic repair mechanisms initiate oligodendrocyte replacement by resident progenitor cells called NG2-glia. To investigate the cellular dynamics of this repair we developed a novel demyelination model by combining intravital myelin imaging with a targeted single-cell ablation technique called 2Phatal. Oligodendrocyte 2Phatal activated a stereotyped degeneration cascade which triggered remyelination by local NG2-glia. Remyelination efficiency was dependent on initial myelin patterning and dynamic imaging revealed rapid repair mechanisms resulting in near-seamless transitions between myelin loss and repair. A subset of morphologically complex NG2-glia executed this remyelination, pointing towards unrecognized functional diversity within this population. Age-related demyelination mirrored the degenerative cascade observed with 2Phatal, while remyelination in aging was defective due to failed oligodendrogenesis. Thus, oligodendrocyte 2Phatal revealed cellular diversity within the oligodendrocyte lineage and uncovered novel forms of rapid remyelination.


2020 ◽  
Vol 41 (6) ◽  
pp. E43-E44
Author(s):  
A. Hagiwara ◽  
M. Hori ◽  
C. Andica ◽  
O. Abe ◽  
S. Aoki

2020 ◽  
Vol 338 ◽  
pp. 108687 ◽  
Author(s):  
Gerhard S. Drenthen ◽  
Walter H. Backes ◽  
Albert P. Aldenkamp ◽  
R. Jeroen Vermeulen ◽  
Sylvia Klinkenberg ◽  
...  

2020 ◽  
Vol 87 (5) ◽  
pp. 710-724 ◽  
Author(s):  
Russell Ouellette ◽  
Gabriel Mangeat ◽  
Ildiko Polyak ◽  
Marcel Warntjes ◽  
Yngve Forslin ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document