Reconstructing summer upper-level flow in the northern Rocky Mountains using an alpine larch tree-ring chronology

Mid-latitude mesoscale weather during the climatological summer is strongly influenced by fluctuations in synoptic-scale circulation patterns. Previous research has linked Arctic amplification to alterations in summer synoptic climatology, leading to more extreme weather events in the mid-latitudes. In this study, seasonal (JJA) upper-level (500 hPa) atmospheric flow is reconstructed in the mid-latitudes using an alpine larch Larix lyallii Parl. tree-ring chronology sampled from western Montana. Significant relationships were found between alpine larch radial growth and upper-level flow patterns derived from the North American Regional Reanalysis dataset (1979-2015). Meridional and zonal flows that manifest in ridging are associated with enhanced radial growth of alpine larch (i.e. meridional flow west [r = 0.504, p = 0.001] and zonal flow north [r = 0.642, p < 0.001] of the study site). Meridional and zonal flows associated with troughing result in decreased radial growth (i.e. meridional flow east [r = -0.497, p = 0.001] and zonal flow south [r = -0.584, p < 0.001] of the study site). Using the leave-one-out method, a linear regression model was calibrated and verified between a principal component analysis score derived from measurements of upper-level flow in western North America and alpine larch tree growth. The 444 yr climate reconstruction of summer 500 hPa flow suggests that ridging is becoming more intense over the western United States and Canada since the 1980s.

A First Generation Heterobasidion Hybrid Discovered in Larix lyalli in Montana

On September 25, 2010, a wood sample was collected from an entirely decayed root ball of an alpine larch (Larix lyallii Parl.), 10 cm in diameter at breast height, recently downed, but still green. No attempts were made to determine whether the decay progressed into the stem. The discovery occurred in a stand in the Bitterroot Mountains, south of Darby, Montana (elev. 2,530 m; 45.893528° N, 114.278322° W). Several adjacent alpine larches were either dead or displayed thin crowns, and an old Heterobasidion basidiocarp was found on the decayed root ball of a neighboring dead tree, suggesting the presence of a root disease pocket. The stand is mature and composed of alpine larch, whitebark pine (Pinus albicaulis Engelm.), and a few subalpine firs (Abies lasiocarpa (Hooker) Nuttall), but only larches were symptomatic. No stumps were visible, and the site is in a designated wilderness area characterized by minimal forest management. Wood chips displaying a white rot with bleached speckles were plated on 2% malt agar, and cultures displaying the typical Heterobasidion anamorph (Spiniger meineckellus) were visible after 7 days. DNA was extracted from two distinct cultures, and the sequences of three nuclear loci, namely the internal transcribed spacer, the elongation factor 1-alpha, and the glyceraldehyde 3-phosphate dehydrogenase, were analyzed. The sequence of the mitochondrial ATPase was also sequenced. All loci were amplified using the primers indicated in Linzer et al. (2). Sequences of all three nuclear loci (GenBank Accession Nos. KF811480 to 82) unequivocally indicated both isolates to be first generation hybrids between H. irregulare (Underw.) Garbel. & Otrosina and H. occidentale Otrosina & Garbel. Cumulatively, sequences were heterozygous at over 40 positions in all three loci, and for the presence of two indels (one in ITS, one in EF 1-alpha). Polymorphisms and indels indicated alleles from both species were present in these heterokaryotic (ploidy n+n) isolates. The mitochondrial ATPase (KF811483 to 84) indicated instead the cytoplasm belonged to H. occidentale, suggesting that species was the first to be established in the infected tree and was either dikaryotized by a basidiopsore of the other species, or subject to nuclear re-assortment through di-mon mating with a genotype of H. irregulare. This is the first report of a Heterobasidion sp. in L. lyalli, and it is the second report of a natural Heterobasidion hybrid in North America (1). This finding indicates Alpine larch may be a host for both Heterobasidion species, as described for pine stumps in California (1). Thus, this conifer may have provided a substrate for the hybridization and interspecific gene introgression documented to have occurred before stumps were generated in high frequency by modern forestry practices (2). References: (1) M. Garbelotto et al. Phytopathology 86:543, 1996. (2) R. Linzer et al. Mol. Phylogenet. Evol. 46:844, 2008.

Cone entomofauna of whitebark pine and alpine larch (Pinaceae): potential impact of Leptoglossus occidentalis (Hemiptera: Coreidae) and a new record of Strobilomyia macalpinei (Diptera: Anthomyiidae)

Laboratory and field feeding tests with Leptoglossus occidentalis Heidemann demonstrated that both immature and mature seed bugs can use cones and foliage of whitebark pine, Pinus albicaulis Engelmann, as a food source for 1- to 2-week periods. Damage to unprotected whitebark pine cones by seed bugs ranged from 0.3 to 2.1% of seeds per cone. Total insect damage ranged from 0.4 to 7.1% of seeds per cone. A seed chalcid, Megastigmus Dalman. sp. (Hymenoptera: Torymidae), was documented for the first time on whitebark pine and was found in 4.7% of examined seeds at one site. The larch cone fly, Strobilomyia macalpinei Michelsen, was reared from cones of alpine larch, Larix lyallii Parl., from the Bitterroot Range of Montana. This is the first record of this species in the United States and the first since its description in 1988. Ninety-four percent of a sample of alpine larch cones were damaged by cone fly larvae and 64% contained larvae or puparia.

Isolation, characterizaton, and inheritance of microsatellite loci in alpine larch and western larch

Microsatellite loci or simple sequence repeat loci (SSRs) were isolated in alpine larch (Larix lyallii Parl.) and western larch (Larix occidentalis Nutt.). In total, 14 SSR loci were characterized; two [(TCT)4, A7] came from published Larix DNA sequence data, one (CA)17 was obtained from a partial non-enriched alpine larch total genomic DNA library, and the remaining 11 loci were obtained from larch genomic DNAs enriched for (CA)n repeats. The SSR regions in these clones could be divided into three categories: perfect repeat sequences without interruption, imperfect repeat sequences with interruption(s), and compound repeat sequences with adjacent tandem simple dinucleotides. Eight of the 14 loci analyzed were found to be polymorphic and useful markers after silver-staining polyacrylamide gel electrophoresis. In addition, several SSR primers developed for alpine larch were able to successfully amplify polymorphic loci in its related species, western larch, and among other closely related taxa within the Larix genus. The inheritance of microsatellite loci was verified by analysis of haploid megagametophyte and diploid embryo tissues of progeny obtained from controlled crosses between western larch and alpine larch. All microsatellite loci analyzed had alleles that segregated according to expected Mendelian frequencies. Two species-specific markers (UAKLly10a and UAKLla1) allow easy and rapid identification of specific genetic entry of alpine larch and western larch at any stage in the sporophyte phase of the life cycle. Therefore, these markers are efficient in identifying the parental species and to validate controlled crosses between these two closely related species. These results are important in tree improvement programs of alpine larch and western larch aimed at producing genetically improved hybrid stock for reforestation in Western Canada and U.S.A.Key words: database search, enriched library, inheritance, Larix, microsatellites, simple sequence repeats, PCR.

The dendrochronological characteristics of alpine Larch

Ring-width chronologies have been developed for alpine larch (Larixlyallii Pari.) at six tree-line sites in the Banff–Kananaskis area of the southern Canadian Rockies. Evaluation of all chronologies confirms that alpine larch ring-width series have higher mean sensitivities, lower autocorrelation, and greater common variance than series from other tree species growing at tree line in this area. Missing and very narrow rings created cross-dating difficulties at all sites but were most problematic for the sites along the Continental Divide. Up to 1% of the rings were missing from entire chronologies, but for specific years, up to 77% of the rings were missing at one site. Marker (narrow) rings were present at all sites for the years 1610, 1654, 1715, 1720, 1723, 1752, 1799, 1824, 1842, 1844, 1915, 1925, 1951, and 1971. Tree growth rates were reduced for the periods 1675–1710, 1815–1850, and 1960–1978 whereas increased growth occurred during 1650–1680, 1750–1800, 1850–1900, and 1920–1950. The only factor that could affect all sites over such a broad geographic area is climate. Principal components analysis indicated that the first eigenvector derived from the six chronologies accounted for 70.7% of their common variance and the first three components accounted for 91.6%. The high correlation between the six first principal component and standard chronologies suggested that either method may be used for standardizing tree-ring data from alpine larch.