Questing behavior of adult Amblyomma americanum (L.) in a laboratory setting

Tick-borne diseases are on the rise globally; however, information is lacking about tick questing behavior. In this laboratory study, we explored tick preferences for stem type (plastic grass, wooden, and metal), questing height, and head orientation. Using 60 Amblyomma americanum adults over three 72-hour replicates, we determined that 21.7% of ticks quested at any given time and that ticks exhibited a strong preference to quest with their heads oriented downwards, irrespective of stem type. Individual ticks tended to quest on only one stem in this study, and a maximum of three. Nonetheless, ticks appeared to prefer questing on wooden and plastic grass stems over metal stems. We did not find an effect of time of day on tick questing rates. Increased understanding of tick questing behavior can improve vector control efforts.

Evaluation of Remote Categorical Loudness Scaling

Purpose: The aims of this study were to (a) demonstrate the feasibility of administering categorical loudness scaling (CLS) tests in a remote setting, (b) assess the reliability of remote compared with laboratory CLS results, and (c) provide preliminary evidence of the validity of remote CLS testing. Method: CLS data from 21 adult participants collected in a home setting were compared to CLS data collected in a laboratory setting from previous studies. Five participants took part in studies in both settings. Precalibrated equipment was delivered to participants who performed headphone output level checks and measured ambient noise levels. After a practice run, CLS measurements were collected for two runs at 1 and 4 kHz. Results: Mean headphone output levels were within 1.5 dB of the target calibration level. Mean ambient noise levels were below the target level. Within-run variability was similar between the two settings, but across-run bias was smaller for data collected in the laboratory setting compared with the remote setting. Systematic differences in CLS functions were not observed for the five individuals who participated in both settings. Conclusions: This study demonstrated that precise stimulus levels can be delivered and background noise levels can be controlled in a home environment. Across-run bias for remote CLS was larger than for in-laboratory CLS, indicating that further work is needed to improve the reliability of CLS data collected in remote settings. Supplemental Material https://doi.org/10.23641/asha.17131856

Het gebruik van wearables bij chronische stress en burn-out

The use of wearables in chronic stress and burnout In recent years, stress-related disorders have become increasingly common in the labor force. Prevention and treatment of chronic stress and burnout are important as this affects all walks of life. A literature search through the databases PubMed and Embase revealed that wearables are promising in the prevention and treatment of work-related stress. Wearables are able to monitor stress in a non-invasive and objective way by measuring parameters such as heart rate variability, skin conductance and skin temperature. By giving the user insight into the factors that trigger stress, a decrease in the average stress level can be achieved. Less research has been done on the use of wearables in burnout. In order to find the ideal combination of stress parameters, further studies which also take into account contextual factors, such as exercise and nutrition, cost and ease of use, are needed in- and outside the laboratory setting.

The Design of Sensor and Transducer Learning Media based on Augmented Reality

This study aimed at determining the design stage of the augmented reality-based learning media for sensors and transducers course. This study employed the Research and Development method with the ADDIE model with the stages of analysis, design, development, implementation, and evaluation. This article focuses on the design stage during the process of media development. The developed learning media can be used among vocational students by visualizing the sensor and transducer components like in the real laboratory setting. The design of the developed learning media contained application storyboards and animation storyboards for practicing the six sensors. This augmented reality learning media is designed to support online learning and students’ autonomous learning in the practical course of sensors and transducers.

Chronobiological Effect on the Reproductive Behavior of Chrysomya megacephala (Diptera: Calliphoridae)

The most widely used entomological method of determining the time since death (minimum postmortem interval, mPMI) has been calculating the developmental time of blow flies (Diptera: Calliphoridae) on the deceased body. However, because blow flies are known to be diurnal, nocturnal oviposition has been excluded from standard mPMI calculations. This has been challenged by recent studies demonstrating nocturnal oviposition due to an unknown reason. Therefore, this study investigated the role of chronobiology. We recorded the locomotion amount and pattern of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) under different chronobiological conditions and examined whether Ch. megacephala can oviposit under nighttime conditions in field and laboratory settings. Subjects were found to have a daily activity pattern under normal darkness conditions (12:12 L:D) and under continuous darkness (DD), but they exhibited no pattern under continuous light (LL). Free-running period was approximately 1,341 min/d (22.35 h/d). In the field, no flies were observed during nighttime. Oviposition occurred in the laboratory setting during daytime with no lights and during nighttime with artificial lights. Free-running subjects oviposited in both active and resting periods, with more eggs laid during active than resting periods. The result of this study indicates it is possible to induce oviposition behavior during evening hours on Ch. megacephala. However, this was only observed in the laboratory setting and could only happen during the flies’ subjective day.

Short Communication: Latent Detection of Downy Mildew (Peronospora pisi) in Bioassays against Pisum sativum

Downy mildew of peas is caused by the obligate parasite Peronospora pisi, which occurs sporadically throughout temperate pea-growing regions across the world. To screen pea lines against this biotrophic pathogen, a suitable and reproducible in vitro method using living plant material is required. Field screening can be influenced by environmental factors, thus giving variable results. The aim of this study was to develop a method that could reliably be used to screen pea cultivars against P. pisi in a laboratory setting. A range of bioassays were used to test various methods of inoculation, utilizing sporangia and naturally infested soil. Latent infection was achieved by planting seeds in soil collected from a site with a known history of P. pisi infection and directly inoculating young pea plants with sporangia. Out of the 108 plants which survived the experimental period, only two plants expressed visible signs of disease; however, through a two-step nested PCR process we detected latent infection in 24 plants. This research highlights the importance of considering the presence of latent infection when screening pea lines against downy mildew.

Lost in transition: The effects of transitioning between firearms and electronic control devices (ECDs) on perception-response times (PRTs)

The research described in this article tested the perception-response times for experienced police officers to transition from a firearm to a TASER and from a TASER to a firearm. The theoretical models and police training on use of force have largely ignored the temporal space between force modalities. Escalating through force modalities has by default been treated as equivalent, in task and timing, to deescalating through force modalities. This study employed a randomized controlled experiment using a police firearms training simulator and 139 active law enforcement officers. The average perception-response time for transition from a TASER to a firearm was 2.49 seconds for experienced police officers in response to an anticipated visual stimulus in a laboratory setting. The average perception-response time for transition from a firearm to a TASER was 4.7 seconds for experienced police officers in a response to an anticipated visual stimulus in a laboratory setting. 70% of the officers that participated in the study had never participated in department training that required them to transition between a firearm and a TASER. The findings demonstrate that moving from TASER to firearm and from firearm to TASER are not equivalent tasks. In the case of firearms and TASERs, it is significantly faster to move up the force continuum—from TASER to firearm—than it is to move down the force continuum. This research has implications for police training, tactics, policy, research, and post hoc investigations involving the use or potential use of force.