stationary phase cell
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2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Sanket Pramod Anaokar ◽  
Ravindra Kodali ◽  
Benjamin Jonik ◽  
Alexiy Nikiforov ◽  
Ida Lager ◽  
...  

2016 ◽  
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Sujata S. Chaudhari ◽  
Vinai C. Thomas ◽  
Marat R. Sadykov ◽  
Jeffrey L. Bose ◽  
Daniel J. Ahn ◽  
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Science ◽  
2009 ◽  
Vol 325 (5947) ◽  
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D.-C. Oh ◽  
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1990 ◽  
Vol 36 (9) ◽  
pp. 657-663 ◽  
Author(s):  
Matthew A. Vierkant ◽  
Daniel W. Martin ◽  
James R. Stewart

Since poly-β-hydroxybutyrate (PHB) inclusions have been reported in only a few strains of Acinetobacter grown on β-hydroxybutyrate, it was of interest to study comparatively the production of this lipid reserve in eight different acinetobacters. Three lines of evidence for PHB production were used: spectrophotometric analysis, infrared analysis, and electron microscopy. The spectrophotometric method showed that all eight strains produce PHB in stationary phase, the amounts ranging from 0.55 to 10.7 fmol/cell. PHB production was maximum after 1 h of growth in batch culture, with the amounts declining by 2 h and returning to near stationary phase cell levels in 4 h. Infrared spectra of purified PHB from the eight strains matched the spectrum of a commercial sample of PHB. In electron micrographs, the PHB reserves were visible as small electron-transparent bodies delineated by ultramembranes. PHB production in four strains (bath cultures) varied in response to exogenous glucose or xylose, with maximum production occurring over a range of 8–17 mM monosaccharide. Glucose was transported into cells by all strains in amounts that ranged from 0.5 to 1.6 fmol/cell, and 0.1–2.2% of that glucose was converted into PHB. Glucose transport was inhibited by cyanide. Key words: poly-β-hydroxybutyrate, acinetobacters.


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