Characterization of Stem Cells and Immune Cells in Preterm and Term Mother’s Milk

Background: Human milk is known to be rich in cellular components, including stem cells and immune cells. However, the dynamics of these cellular components at different lactation stages, and the differences between milk for preterm and term infants, are poorly understood. Research aim: To identify changes in the cellular components of human milk at different lactation stages, and to explore the associations of these changes with maternal and infant characteristics. Methods: Forty mothers of newborns of different gestational ages were enrolled. Colostrum, transitional, and mature milk samples were collected. Stem cell and immune cell molecule markers were detected using flow cytometry. Pluripotent genes (SOX2, NANOG, OCT4, and KLF4) were detected via quantitative real-time PCR. Results: Human milk contained some stem cells but more immune cells. The percentages of hemopoietic stem cells were significantly higher in mature milk than in colostrum, and the percentages of total immune cells were lower in mature milk than in colostrum. The percentages of hemopoietic stem cells in colostrum and transitional milk were influenced by gestational age. Some minor differences in the cell composition of human milk could be explained by maternal body mass index, the mode of delivery, and parity. Conclusion: Our results again confirmed that human milk contains stem cells. Additionally, the percentages of hemopoietic stem cells and major immune cells changed dynamically at different lactation stages and were associated with gestational age at delivery.

PUNCTURE TREATMENT OF SINUSITIS IN PATIENTS WHITH CYTOPENIA AFTER HEMOPOIETIC STEM CELLS TRANSPLANTATION

Introduction: Sinusitis is one of the most common infectious complications at the stages of hematopoietic stem cell transplantation. Diagnosis and treatment patients whith cytopenia often causes difficulties for otorhinolaryngologists. Objective: To analyze the possibility of puncture of the maxillary sinuses in patients whith cytopenia after hemopoietic stem cells transplantation. Materials and methods. The case histories of 350 patients whith rhinosinusitis, who received treatment at the Raisa Gorbacheva Memorial Research Institute of Children Oncology, Hematology and Transplantation from January 2017 to June 2018 were analyzed. Of these patients, 41 (11%) (11 – 62 y.o.) underwent puncture of the maxillary sinuses. The control group included 59 immunocompetent patients undergoing puncture treatment for sinusitis. Results: The clinical picture of sinusitis in patients with cytopenia was different from the classical one. In cases of severe anemia (<80 g/l), paleness of the mucous membranes of the nasal cavity prevailed; with extremely severe neutropenia (<0.5 x 109/l), and leukopenia (<1.0 x 109/l) in the vast majority of patients, the nasal discharge was mucopurulent. 10% patients with drug immunosuppression and agranulocytosis have pronounced edema and hyperemia of nasal soft tissues. In 19.5% patients, who underwent puncture, severe thrombocytopenia (<25 x 109/l) was observed. In 3 (7%) patients, significant bleeding was observed after puncture. In punctate we observed Str. Viridans (26.8%), Pseudomonas spp. (14.6%), and Klebsiella pneumonia (12.2%) of cases. Conclusion: Sinusitis with cytopenia is prone to atypical course. Symptoms in these conditions can vary from scanty to pronounced. Puncture of the maxillary sinuses in such patients can be performed according to indications.

Evolutionary intraembryonic origin of vertebrate hematopoietic stem cells in the elasmobranch spleen

The electric ray (Torpedo Marmorata Risso) provides an animal model for the detection of early intraembryonic hemopoietic stem cells in sea vertebrates. The spleen of this bone-marrowless vertebrate appears to be the major site of hemopoietic stem cell differentiation during development and in adulthood. Splenic development in this species was investigated and hemopoietic stem cells were detected in this organ by immunocytochemistry utilizing CD34 and CD38 antibodies. At stage I (2-cm-long embryos with external gills), the spleen contains only mesenchymal cells. At stage II (3-4 cm-long embryos with a discoidal shape and internal gills), an initial red pulp was observed in the spleen, without immunostained cells. At stage III (10-11-cm-long embryos), the spleen contained well-developed white pulp, red pulp and ellipsoids. Image analysis at stage III showed four cell populations, i.e. CD34+/CD38-, CD34+/CD38+, CD34-/CD38+, and CD34-/CD38- cells. The present findings, obtained from an elasmobranch, indicate that the CD34 and CD38 phenotypes are conserved through vertebrate evolution.

Srsf2 P95H initiates myeloid bias and myelodysplastic/myeloproliferative syndrome from hemopoietic stem cells

Key Points Srsf2 P95H/+ mutation within hemopoietic stem cells is required to initiate myeloid-biased hemopoiesis. Mutation of Srsf2 is sufficient to initiate the development of MDS/MPN in vivo in the setting of native hemopoiesis.

LIFE SPAN PREDICTION AT METASTATIC NON-SMALL CELL LUNG CANCER

Individual parameters of circulating hemopoietic stem cells (HSC) lymphoid origin were measured by cytofluorometry before treatment of patients with metastatic non-small cell lung cancer and were retrospectively compared with individual life span's (LS). The possibility of poor prognosis of treatment's results (LS

Committed Hemopoietic Progenitors, Not Stem Cells, are the Principal Responders to Hox Gene Transduction

ABSTRACTAs hemopoietic stem cells differentiate, their proliferative lifespan shortens by unknown mechanisms. Homeobox cluster (Hox) genes have been implicated by their enhancement of self-renewal when transduced into hemopoietic cells, but gene deletions have been inconclusive because of functional redundancy. Here we enforced HOXB4 expression in purified precursor stages, and compared responses of early stages expressing the endogenous genes with later stages that did not. Contrary to the prevalent view that transduced Hox genes enhance the self-renewal of hemopoietic stem cells, stem cells or their multipotent progeny expressing the endogenous genes showed little response. Instead, immortalization, extensive self-renewal and acquired reconstituting potential occurred in committed erythroid and myeloid progenitors where the endogenous genes were shutting down. The results change our understanding of the stages affected by exogenous HOX proteins and point to shutdown of the endogenous genes as a principal determinant of the shortened clonal lifespans of committed progenitor cells.