Fruit host-dependent fungal communities in the microbiome of wild Queensland fruit fly larvae

Bactrocera tryoni (Froggatt), the Queensland fruit fly (Qfly), is a highly polyphagous tephritid fly that is widespread in Eastern Australia. Qfly physiology is closely linked with its fungal associates, with particular relationship between Qfly nutrition and yeast or yeast-like fungi. Despite animal-associated fungi typically occurring in multi-species communities, Qfly studies have predominately involved the culture and characterisation of single fungal isolates. Further, only two studies have investigated the fungal communities associated with Qfly, and both have used culture-dependant techniques that overlook non-culturable fungi and hence under-represent, and provide a biased interpretation of, the overall fungal community. In order to explore a potentially hidden fungal diversity and complexity within the Qfly mycobiome, we used culture-independent, high-throughput Illumina sequencing techniques to comprehensively, and holistically characterized the fungal community of Qfly larvae and overcome the culture bias. We collected larvae from a range of fruit hosts along the east coast of Australia, and all had a mycobiome dominated by ascomycetes. The most abundant fungal taxa belonged to the genera Pichia (43%), Candida (20%), Hanseniaspora (10%), Zygosaccharomyces (11%) and Penicillium (7%). We also characterized the fungal communities of fruit hosts, and found a strong degree of overlap between larvae and fruit host communities, suggesting that these communities are intimately inter-connected. Our data suggests that larval fungal communities are acquired from surrounding fruit flesh. It is likely that the physiological benefits of Qfly exposure to fungal communities is primarily due to consumption of these fungi, not through syntrophy/symbiosis between fungi and insect ‘host’.

VALIDATION OF THE MALAY-TRANSLATED EXPECTANCY VALUE MODEL QUESTIONNAIRE AND MULTI-DIMENSIONAL SPORTSPERSONSHIP ORIENTATIONS SCALE AMONG MALAYSIAN ADOLESCENCE FIELD HOCKEY PLAYERS

This study presents an overview of Malaysian adolescent field hockey players’ expectancy beliefs, subjective values and sportspersonship attitude. We aimed to translate and validate two questionnaires i.e., Expectancy Value Model Questionnaire (EVMQ) and Multi-dimensional Sportspersonship Orientations Scale (MSOS) to Malay language. The EVMQ and MSOS were translated into the Malay language using the back translation method. A total of 230 respondents (15.40 ± 2.17 years) were recruited for this study. The result of analyses are EVMQ-M (M = 5.38 ± .95) and MSOS-M (M = 3.64 ± .40) respectively. Content validity was checked by experts in the fields for content conformity and culture bias whereas face validity was confirmed by ten participants. Both questionnaires showed high internal consistency (reliability); EVMQ-M (α = .94) and MSOS-M (α = .82). Data fits were checked using the Structural Equation Model; EVMQ-M (χ² (df = 39) = 84.07, CFI = .97, GFI = .94, TLI = .95, RMSEA = .07, ChiSq/df = 2.16) and MSOS-M (χ² (df = 146) = 321.76, CFI = .92, GFI = .87, TLI = .90, RMSEA = .07, ChiSq/df = 2.20) also showed good construct validity. The translated Malay version EVMQ-M and the MSOS-M are reliable and valid instruments to assess Malaysian adolescent field hockey players.

Cross-cultural literary translation strategies within a Maltese bilingual context

The process of literary translation includes the source culture-specific elements that constitute an integral part of the source text. This paper aims to identify and analyse various translation strategic processes that could be adopted in translating cultural factors within the parameters of a Maltese bilingual, but not necessarily bicultural, context. Each of the suggested strategic procedures is presented in useful flow-chart formats, varying from source language/source culture to target language/target culture bias approach in order to keep cultural losses to a minimum whilst maximising cultural gains and, therefore, to make the transformation of the source text into the target text successful. Such flow-charts are aimed to provide the literary translator with a rapid means of achieving an adequate and satisfying suggested solution for a quality cross-cultural transposition of the cultural elements encountered within a bilingual context. In certain instances, it is also suggested that some strategies are used concurrently with others. To achieve this aim, an extended practical translation exercise by the author himself is used. This paper also helps to strengthen further both the level of research in narrative translation studies in general, and the research done in Maltese narrative literary translation from a cultural point of view.

Competition during enrichment of pathogenicEscherichia colimay result in culture bias

Deadly outbreaks and illnesses due to Shiga toxin-producing Escherichia coli (STEC) occur worldwide; however, the cultivation methods required for adequate monitoring and traceback investigations are inefficient at best. Detection of STEC relies heavily on enrichment; yet no standard media or protocols exist. Furthermore, whether enrichment may bias detection of multiple STEC serogroups from complex samples is unknown. Thus, 14 STEC strains of serogroups O157 and the top six non-O157s (O26, O45, O103, O111, O121, and O145) were enriched in pairs for 6–78 h in broth and evaluated by quantitative polymerase chain reaction (qPCR). Here we show that a conventional 6-h enrichment protocol did not result in intra-species culture bias for the isolates tested. However, subsequent enrichments often produced biased cultures, with differences in the qPCR gene copy number ≥2 log10apparent in 12%, 38%, and 52% of competitions after 30, 54, and 78 h of consecutive enrichments, respectively. Some strains were able to prevail and (or) out-compete the opponent strain in 100% of competitions. Our results suggest that culture bias should be considered and (or) evaluated further due to the potential implications during routine pathogen screening and outbreak investigations.

Structure and specificity of novel aminopeptidase from marine sediment Archaea

The Earth's microbial diversity remained largely unexplored until recent developments in DNA sequencing. Novel methods enabled us to access genomic information of uncultured microbial organisms and create hypotheses about their metabolic capabilities. These predictions primarily rely on the sequence similarity between a novel protein and characterized proteins. Such an approach introduces a "culture" bias: the well-understood proteins come from a set of laboratory-grown bacteria, while novel microbial proteins are obtained from a variety of environments, including the most extreme. One such niche is ocean sediment – an unexplored ecosystem that plays important roles in geochemical cycles. Single-cell genomics targeting sedimentary populations identified four new archaeons encoding putative intra- and extra-cellular proteases [1]. This discovery suggests that heterotrophic marine Archaea evolved to degrade detrital proteins and might contribute to global carbon cycling. The novel proteases share some sequence similarity with well-known protein-degrading enzymes, but generally are distant homologs. Thus, functional screening is necessary to validate sequence-based predictions. One of the proteases shares sequence similarity with S15 peptidases, cocaine esterases and α-amino acid ester hydrolases (AEH). Phylogeny indicates that the gene is of bacterial origin. Enzymatic assays reveal α-aminopeptidase activity towards dipeptides with a preference for a small, L-configured hydrophobic residue at the N-terminus. The crystal structure shows a homotetrameric, self-compartmentalizing enzyme with four independent active sites localized inside the oligomeric assembly accessible from the internal channel. The active site contains a serine protease triad (Ser-His-Asp) and a cluster of negatively charged residues that bind the N-terminal NH3+ group of the substrate molecule. Therefore, the observed activity suggests that the enzyme (designated as AP TA1) may act on di- or tri-peptides produced during extracellular degradation and subsequently imported to the cell. As a close homolog of AEHs, it is also possible that AP TA1 might participate in the synthesis of yet-to-be-discovered secondary metabolites. Supported by NIH GM094585, DOE/BER DE-AC02-06CH11357 & C-DEBI 36202823 & 157595.

Impact of Political and Cultural Factors on Online Education in Africa: the Strategies to Build Capabilities

Recently the concept of online education has received considerable attention worldwide; however, its low success rate in Africa warrants further investigation. The purpose of this study is to examine the impact of political and cultural factors on online education. For the purpose of the study, the political factor constitutes government support, technological infrastructure and trained instructors, whereas the cultural factor focuses on gender bias, culture bias and language barrier of learners. Drawing on the theory of source-position-performance, we argue that source (i.e., online education) should be promoted in rural areas as usages of mobile technologies and cellphones are more than computers, and that online education leads to competitive advantage. Finally, we propose a couple of strategies to build capability.

Bimusicalism: The Implicit Dual Enculturation of Cognitive and Affective Systems

ONE PROMINENT EXAMPLE OF GLOBALIZATION AND MASS cultural exchange is bilingualism, whereby world citizens learn to understand and speak multiple languages.Music, similar to language, is a human universal, and subject to the effects of globalization. In two experiments, we asked whether bimusicalism exists as a phenomenon, and whether it can occur even without explicit formal training and extensive music-making. Everyday music listeners who had significant exposure to music of both Indian (South Asian) and Western traditions (IW listeners) and listeners who had experience with only Indian or Western culture (I or W listeners) participated in recognition memory and tension judgment experiments where they listened to Western and Indian music. We found that while I and W listeners showed an in-culture bias, IW listeners showed equal responses to music from both cultures, suggesting that dual mental and affective sensitivities can be extended to a nonlinguistic domain.