Intrinsic brainstem circuits comprised of Chx10-expressing neurons contribute to reticulospinal output in mice

Glutamatergic reticulospinal neurons in the gigantocellular reticular nucleus (GRN) of the medullary reticular formation can function as command neurons, transmitting motor commands to spinal cord circuits to instruct movement. Recent advances in our understanding of this neuron-dense region have been facilitated by the discovery of expression of the transcriptional regulator, Chx10, in excitatory reticulospinal neurons. Here, we address the capacity of local circuitry in the GRN to contribute to reticulospinal output. We define two sub-populations of Chx10-expressing neurons in this region, based on distinct electrophysiological properties and somata size (small and large), and show that these populations correspond to local interneurons and reticulospinal neurons, respectively. Using focal release of caged glutamate combined with patch clamp recordings, we demonstrated that Chx10 neurons form microcircuits in which the Chx10 local interneurons project to and facilitate the firing of Chx10 reticulospinal neurons. We discuss the implications of these microcircuits in terms of movement selection.

Response bias reveals the role of interhemispheric inhibitory networks in movement preparation and execution

Human movement is influenced by various cognitive processes, such as bias, that dynamically shape competing movement representations. However, the neurophysiological mechanisms underlying the effects of bias on movement selection across the lifespan remains poorly understood.Healthy young (n = 21) and older (n = 20) adults completed a choice reaction time task necessitating left- or right-hand responses to imperative stimuli (IS). Response bias was manipulated via a cue that informed participants a particular response was 70% likely (i.e., the IS was either congruent, or incongruent, with the cue); biasing was either fixed for blocks of trials (block-wise bias) or varied from trial-to-trial (trial-wise bias). As well as assessing the behavioural manifestations of bias, we used transcranial magnetic stimulation to determine changes in corticospinal excitability (CSE) and short- and long-interval interhemispheric inhibition (SIHI, LIHI) during movement preparation and execution. Participants responded more quickly, and accurately, in congruent compared to incongruent trials. CSE decreases occurred in both hands following the cue, consistent with the ‘inhibition for impulse control’ hypothesis of preparatory inhibition. In contrast, IHI modulations occurred in a hand-specific manner. Greater SIHI was observed during movement preparation in the hand biased away from, compared to the hand biased towards, the cue; furthermore, greater SIHI was observed during movement execution in the hand biased towards the cue when it was not required to respond (i.e., incongruent trial) compared to when it was required to respond (congruent trial). Additionally, during the movement preparation period, the LIHI ratio of the hand biased towards, compared to the hand biased away from, the cue was greatest when the cue varied trial-by-trial. Overall, the IHI results provide support for the ‘inhibition for competition resolution’ hypothesis, with hand specific modulation of inhibition during movement preparation and execution.

Local brainstem circuits contribute to reticulospinal output in the mouse

ABSTRACTGlutamatergic reticulospinal neurons in the gigantocellular reticular nucleus (GRN) of the medullary reticular formation can function as command neurons, transmitting motor commands to spinal cord circuits. Recent advances in our understanding of this neuron-dense region have been facilitated by the discovery of expression of the transcriptional regulator, Chx10, in excitatory reticulospinal neurons. Here, we address the capacity of local circuitry in the GRN to contribute to reticulospinal output. We define two sub-populations of Chx10-expressing neurons in this region, based on distinct electrophysiological properties and somata size (small and large), and show that these correspond to local interneurons and reticulospinal neurons, respectively. Using focal release of caged-glutamate combined with patch clamp recordings, we demonstrated that Chx10 neurons form microcircuits in which the Chx10 interneurons project to and facilitate the firing of Chx10 reticulospinal neurons. We discuss the implications of these microcircuits in terms of movement selection.SIGNIFICANCE STATEMENTReticulospinal neurons in the medullary reticular formation play a key role in movement. The transcriptional regulator Chx10 defines a population of glutamatergic neurons in this region, a proportion of which have been shown to be involved in stopping, steering, and modulating locomotion. While it has been shown that these neurons integrate descending inputs, we asked whether local processing also ultimately contributes to reticulospinal outputs. Here, we define Chx10-expressing medullary reticular formation interneurons and reticulospinal neurons, and demonstrate how the former modulate the output of the latter. The results shed light on the internal organization and microcircuit formation of reticular formation neurons.

Functional Connectivity and Classification of Actual and Imaginary Motor Movement

Imaginary Motor movement is an utmost important for the designing of brain computer interface to assist the individual with physically disability. Brain signals associated with actual motor movement include the signal for muscle activity whereas in case of imaginary motor movement actual muscle movement is not present .Authors have investigated the similarity/dissimilarity between the eeg signals generated in both the cases along with the baseline activity. To instruct the brain computer interface signals generated by electrodes of EEG must resemble with actual motor movement. Selection of electrodes placement plays an important role for this purpose. In this study major four regions of the brain has been covered frontal, temporal, parietal and occipital region of the scalp and features are extracted from the signals are standard deviations, kurtosis, skew and mean. Support Vector Machine is used for the classification between actual and imaginary motor movement along with differentiation between baseline and imaginary motor movement and actual motor movement at 14 different electrodes positions. Statistical performances of the classifier have been evaluated by computing sensitivity, specificity and accuracy. The location involved to achieve maximum accuracy for the classification of motor movements (actual and imaginary) and no motor movement is at frontal, temporal and parietal region

Electrocorticographic dissociation of alpha and beta rhythmic activity in the human sensorimotor system

This study uses electrocorticography in humans to assess how alpha- and beta-band rhythms modulate excitability of the sensorimotor cortex during movement selection, as indexed through a psychophysically-controlled movement imagery task. Both rhythms displayed effector-specific modulations, tracked spectral markers of action potentials in the local neuronal population, and showed spatially systematic phase relationships (traveling waves). Yet, alpha- and beta-band rhythms differed in their anatomical and functional properties, were weakly correlated, and traveled along opposite directions across the sensorimotor cortex. Increased alpha-band power in the somatosensory cortex ipsilateral to the selected arm was associated with spatially-unspecific inhibition. Decreased beta-band power over contralateral motor cortex was associated with a focal shift from relative inhibition to excitation. These observations indicate the relevance of both inhibition and disinhibition mechanisms for precise spatiotemporal coordination of neuronal populations during movement selection. Those mechanisms are implemented through the substantially different neurophysiological properties of sensorimotor alpha- and beta-band rhythms.

Prior Knowledge of Target Direction and Intended Movement Selection Improves Indirect Reaching Movement Decoding

Objective.Previous studies have demonstrated that target direction information presented by the dorsal premotor cortex (PMd) during movement planning could be incorporated into neural decoder for achieving better decoding performance. It is still unknown whether the neural decoder combined with only target direction could work in more complex tasks where obstacles impeded direct reaching paths.Methods.In this study, spike activities were collected from the PMd of two monkeys when performing a delayed obstacle-avoidance task. We examined how target direction and intended movement selection were encoded in neuron population activities of the PMd during movement planning. The decoding performances of movement trajectory were compared for three neural decoders with no prior knowledge, or only target direction, or both target direction and intended movement selection integrated into a mixture of trajectory model (MTM).Results.We found that not only target direction but also intended movement selection was presented in neural activities of the PMd during movement planning. It was further confirmed by quantitative analysis. Combined with prior knowledge, the trajectory decoder achieved the best performance among three decoders.Conclusion.Recruiting prior knowledge about target direction and intended movement selection extracted from the PMd could enhance the decoding performance of hand trajectory in indirect reaching movement.

Basal ganglia output reflects internally-specified movements

How movements are selected is a fundamental question in systems neuroscience. While many studies have elucidated the sensorimotor transformations underlying stimulus-guided movements, less is known about how internal goals – critical drivers of goal-directed behavior – guide movements. The basal ganglia are known to bias movement selection according to value, one form of internal goal. Here, we examine whether other internal goals, in addition to value, also influence movements via the basal ganglia. We designed a novel task for mice that dissociated equally rewarded internally-specified and stimulus-guided movements, allowing us to test how each engaged the basal ganglia. We found that activity in the substantia nigra pars reticulata, a basal ganglia output, predictably differed preceding internally-specified and stimulus-guided movements. Incorporating these results into a simple model suggests that internally-specified movements may be facilitated relative to stimulus-guided movements by basal ganglia processing.