DNA Methylation of Endoglin Pathway Genes in Pregnant Women With and Without Preeclampsia

Objective: We compared blood-based DNA methylation levels of endoglin ( ENG) and transforming growth factor beta receptor 2 ( TGFβR2) gene promoter regions between women with clinically-overt preeclampsia and women with uncomplicated, normotensive pregnancies. Methods: We used EpiTect Methyl II PCR Assays to evaluate DNA methylation of CpG islands located in promoter regions of ENG (CpG Island 114642) and TGFβR2 (CpG Island 110111). Preeclampsia was diagnosed based on blood pressure, protein, and uric acid criteria. N = 21 nulliparous preeclampsia case participants were 1:1 frequency matched to N = 21 nulliparous normotensive control participants on gestational age at sample collection (±2 weeks), smoking status, and labor status at sample collection. Methylation values were compared between case and control participant groups [( ENG subset: n = 20 (9 cases, 11 controls); TGFβR2 subset: n = 28 (15 cases, 13 controls)]. Results: The majority of the preeclampsia cases delivered at ⩾34 weeks’ gestation (83%). Average methylation levels for ENG ([M ± (SD)]; Case Participant Group = 6.54% ± 4.57 versus Control Participant group = 4.81% ± 5.08; P = .102) and TGFβR2 (Case Participant Group = 1.50% ± 1.37 vs Control Participant Group = 1.70% ± 1.40; P = .695) promoter CpG islands did not differ significantly between the participant groups. Removal of 2 extreme outliers in the ENG analytic subset revealed a trend between levels of ENG methylation and pregnancy outcome (Case Participant Group = 5.17% ± 2.16 vs Control Participant Group = 3.36% ± 1.73; P = .062). Conclusion: Additional epigenetic studies that include larger sample sizes, investigate preeclampsia subtypes, and capture methylation status of CpG island shores and shelves are needed to further inform us of the potential role that ENG and TGFβR2 DNA methylation plays in preeclampsia pathophysiology.

A magnetoencephalographic study of longitudinal brain function alterations following carpal tunnel release

We investigate changes in brain function before and after carpal tunnel release. Magnetoencephalography (MEG), during which we recorded somatosensory evoked cortical magnetic fields (SEFs), and a clinical evaluation were performed before surgery and 6 months after. The distance on the vertical axis between the equivalent current dipoles (ECDs) for the first and third digits before surgery was significantly less than after surgery. There were no significant differences in values between the control participant and patients after surgery. In terms of distal motor latency, there was a negative correlation with the distance. The recovery function of the root mean square (RMS) before surgery for the N20m was less suppressed at 10 ms of ISI in patients, compared to controls. There were no significant differences in the RMS values for patients before and after surgery. Our results indicate that treating peripheral nerve lesions, such as in carpal tunnel release, positively modifies brain function.

Controlling a robotic arm for functional tasks using a wireless head-joystick: A case study of a child with congenital absence of upper and lower limbs

Children with movement impairments needing assistive devices for activities of daily living often require novel methods for controlling these devices. Body-machine interfaces, which rely on body movements, are particularly well-suited for children as they are non-invasive and have high signal-to-noise ratios. Here, we examined the use of a head-joystick to enable a child with congenital absence of all four limbs to control a seven degree-of-freedom robotic arm. Head movements were measured with a wireless inertial measurement unit and used to control a robotic arm to perform two functional tasks – a drinking task and a block stacking task. The child practiced these tasks over multiple sessions; a control participant performed the same tasks with a manual joystick. Our results showed that the child was able to successfully perform both tasks, with movement times decreasing by ~40-50% over 6-8 sessions of training. The child’s performance with the head-joystick was also comparable to the control participant using a manual joystick. These results demonstrate the potential of using head movements for the control of high degree-of-freedom tasks in children with limited movement repertoire.

Brain Activation for Knee Movement Measured Days Before Second Anterior Cruciate Ligament Injury: Neuroimaging in Musculoskeletal Medicine

Background Anterior cruciate ligament (ACL) injury has multifactorial causes encompassing mechanical, hormonal, exposure, and anatomical factors. Alterations in the central nervous system also play a role, but their influence after injury, recovery, and recurrent injury remain unknown. Modern neuroimaging techniques can be used to elucidate the underlying functional and structural alterations of the brain that predicate the neuromuscular control adaptations associated with ACL injury. This knowledge will further our understanding of the neural adaptations after ACL injury and rehabilitation and in relation to injury risk. In this paper, we describe the measurement of brain activation during knee extension-flexion after ACL injury and reconstruction and 26 days before a contralateral ACL injury. Methods Brain functional magnetic resonance imaging data for an ACL-injured participant and a matched control participant were collected and contrasted. Results Relative to the matched control participant, the ACL-injured participant exhibited increased activation of motor-planning, sensory-processing, and visual-motor control areas. A similar activation pattern was present for the contralateral knee that sustained a subsequent injury. Conclusions Bilateral neuroplasticity after ACL injury may contribute to the risk of second injury, or aspects of neurophysiology may be predisposing factors to primary injury. Clinical Implications Sensory-visual-motor function and motor-learning adaptations may provide targets for rehabilitation.

Compulsive buying: a field study of mood variability during acquisition episodes

Empirical investigations of mood variability during actual and ‘real time’ shopping episodes are absent from the compulsive-buying (CB) literature. A field experiment was therefore conducted with a participant meeting desired clinical and research requirements for identifying CB and a control subject for comparison purposes. Each participant went on four shopping trips and rated various parameters of mood and self-perception prior to, during and after each shopping trip. Each phase (i.e. pre-shop, shop and post-shop) lasted for 1.5 h, with ratings of mood and self-evaluation taken every 10 min. Results indicate that, although shopping was a guilty pleasure for the CB participant, there were more similarities than differences apparent in terms of mood and self-evaluation between the CB and control participant. The CB participant experienced significant levels of self-dislike during the shopping episodes. In terms of the comparisons between the pre-, during and post-shopping phases, the first 40 minutes of actual shopping seemed to be particularly arousing/exciting for the CB participant. The study is discussed in terms of key methodological requirements for increased validity and reliability in studying CB phenomena and the range of indicated possible cognitive and behavioural interventions.

The Development and Activation of Achievement Goals Within Tennis: II. A Player, Parent, and Coach Intervention

This study acts as a follow-up to a previous investigation into the development and activation of achievement goals within young tennis players (Harwood & Swain, 2001). The project investigated the effects of a season-long player, parent, and coach intervention program on goal involvement responses, self-regulation, competition cognitions, and goal orientations of three junior tennis players. First, each player reported goal involvement, self-regulation, self-efficacy, and perceptions of threat and challenge prior to three ego-involving match situations. Aligned with a matched control participant, each treatment player, with their parents and coach, engaged in educational sessions and cognitive-motivational tasks over a three-month competition and training period. Postintervention, positive directional changes were reported in all players except the control participant. This study reinforces to applied researchers and practitioners the importance and practicability of social-cognitive and task-based interventions designed to facilitate optimal, motivational, and psychological states in high pressure competitive situations.

Amnesic patients' impaired category exemplar production priming

This study examined the effect of varying the number of potential target words on amnesic patients' category exemplar production performance. In Experiment 1, 4 words from each of 6 categories were presented to amnesic patients and normal control participants. This was followed by an indirect task in which each participant produced the first 8 words that came to mind when presented with a category cue. On this task the amnesic patients were impaired. This outcome stands in sharp contrast to most other category exemplar production tasks that have been reported. However, these other paradigms tend to restrict participants' processing during target item presentation while our procedure allowed them to analyze the target words as they chose. Our procedure may have allowed the control participant more opportunity to “cluster” target words from the same category during list presentation and this, in turn, may have given them an advantage at the time of category exemplar production. Therefore, in Experiment 2, only 1 word per category was presented in the target list and only 2 words per category were requested during category exemplar production. Surprisingly, the amnesic patients still exhibited impaired performance. Therefore, it was suggested that perhaps amnesic patients' known inability to perform semantic levels of processing during individual target word presentation may have resulted in impaired priming for categorical features for these patients. (JINS, 1998, 4, 576–583.)

World of Bizcraft

This article sketches the features required of a platform (which I refer to as ‘World of Bizcraft’) that supports virtual worlds dedicated to research and education on business-related topics. Key features include progressivity of content and challenges, which is a standard feature in most educational processes; certification of players’ achievements, rather than the achievements of the players’ characters; the ability to control participant interaction, collaboration and creation of game assets; implementation of induced value, which forms the foundation of experimental research in economics; production functions that capture the realities of real businesses; sophisticated property rights that support complex software-enforced contracts; and comprehensive systems for business reporting.