Humanization of wildlife gut microbiota in urban environments

Urbanization is rapidly altering Earth’s environments, demanding investigations of the impacts on resident wildlife. Here, we show that urban populations of coyotes (Canis latrans) and crested anole lizards (Anolis cristatellus) acquire gut microbiota constituents found in humans, including the gut bacterial lineages most significantly associated with urbanization in humans (e.g., Bacteroides). Comparisons of urban and rural wildlife and human populations revealed significant convergence of the gut microbiota among urban host populations. Remarkably, all microbial lineages found in humans that were overrepresented in urban wildlife relative to rural wildlife were also overrepresented in urban humans relative to rural humans. These results indicate parallel effects of urbanization on human and wildlife gut microbiota and suggest spillover of bacteria from humans into wildlife in cities.

Lessons Learned from Developing Best Management Practices for Urban Tree Care and Wildlife

Urban forests create indispensable habitat for declining wildlife populations. The tree care industry is essential to the viability of urban forests and thus the survival of urban wildlife. At the same time, tree care operations such as tree removal and branch pruning present clear threats to urban wildlife and their habitats. Here we describe the development of a grassroots coalition of arborists and wildlife advocates in the Western United States and the process of charting a path to best management practices and professional training to mitigate the impacts of tree care practices to wildlife. In particular, we describe the unique challenges and opportunities that arose through this multi-disciplinary process and build a case for the benefits of uniting diverse communities of practice around complex urban ecological problems. We finish by laying out recommendations to the international arboriculture and urban forestry practitioner and research communities.

Wildlife Affordances of Urban Infrastructure: A Framework to Understand Human-Wildlife Space Use

Landscape affordances, what the environment offers an animal, are inherently species-specific to the extent that each taxon has unique needs and responses to landscape characteristics. Wildlife responses to landscape features range on a continuum from avoidance to attraction and quantifying these habits are the backbone of wildlife movement ecology. In anthropogenically modified landscapes, many taxa do not occupy areas heavily influenced by humans, while some species seem to flourish, such as coyotes (Canis latrans) and pigeons (Columba livia). Sufficient overlap in landscapes designed for human purposes (e.g., freeway underpasses, channelized waterways, and cemeteries) but which are also suitable for wildlife (e.g., by providing sources of food, shelter, and refuge) underlies wildlife persistence in urban areas and is increasingly important in the world's largest metropoles. Studying these overlapping worlds of humans and wildlife in cities provides a rich foundation for broadening human perceptions of cities as ecosystems that exhibit emergent hybridity, whereby certain anthropogenic features of urban landscapes can be used by wildlife even as they maintain their utility for humans. By examining scaling dynamics of the infrastructural signature, the phenomena of urban wildlife movement patterns conforming to the shapes of human infrastructural forms, we hope to expand on prior research in wildlife landscape ecology by stressing the importance of understanding the overlapping worlds of humans and wildlife. Further knowledge of the urban ecological commons is necessary to better design cities where emergent hybridity is leveraged toward the management goals of reducing human wildlife conflict and promoting biodiversity.

Common notions and composite collaborations: Thinking with Spinoza to design urban infrastructures for human and wild cohabitants

This paper explores the ways in which we might construct urban environments that are responsible to the needs of more than just human cohabitants. Drawing on Spinoza’s common notion and attentive to the possibilities of socio-natures that both construct and respond to the habitat needs of urban wildlife, I look at how urban design and wildlife habitat might be thought and planned together as a human/non-human composite, invoking a complex spatial and temporal choreography which serves divergent needs. Drawing on examples of urban design in Toronto, Canada, this paper offers a way to think of the city as a composite body in Spinoza’s terms, to become open to an awareness of the city as a composition of forces—a choreography of bodies that are constantly interweaving and overflowing imagined boundaries, struggles that are fought as much over time as space, the accommodation of the temporalities and spatialities of other life processes, other rhythms and cycles that would, without a recalibration, sync uneasily with the pacing and spacing of human requirements.

Pigeons and People: Resource Ecology and Human Dimensions of Urban Wildlife

<p>Urban areas and human populations are growing. Cities provide highly modified habitat for species that can adapt their feeding and other behaviours. The growth of urban landscapes and human populations may result in an increase in human-wildlife conflict. Businesses which prepare and sell food (food establishments) may be more likely to encounter conflict with urban wildlife, which may lead to negative attitudes towards urban wildlife. Negative attitudes towards wildlife could create polarised communities and possibly affect the success of environmental initiatives. This study sought to understand (1) how feral pigeons use urban environments and the resources key to their distribution and congregation; (2) whether feral pigeons are food limited in Wellington City; and (3) how the interactions of owners and managers of food establishments with feral pigeons influence their attitudes to feral pigeons. I used 8 transects through the central City which covered a representative sample of urban habitats, including the central business district, green space, and waterfront to estimate resource selection. Bird capture and banding were used to determine feral pigeon condition at a range of sites across the City and included a mix of high, medium and low anthropogenic fed sites. A written survey of owners and managers of food establishments in Wellington was conducted to evaluate attitudes to feral pigeons (n = 62). Feral pigeon resource selection is mainly influenced by people and where they choose to eat (∆AIC ≤ W = 0.999), such as sites with outdoor seating where people may directly feed feral pigeons. However, once a site has been selected, areas with tertiary vegetation and disposed food (W = 0.324 and W = 0.297) are the most likely to attract larger flocks of feral pigeons (although a number of other variables also influence flock size, such as availability of freshwater). Feral pigeons do not appear to be food limited in Wellington as condition was not significantly different between sites (n=48, body condition, (body mass/tarsus length) Kruskal-Wallis = 2.06, p = 0.36; keel condition, Kruskal-Wallis = 0.7283, p = 0.6948; feather condition Kruskal-Wallis = 2.7943, p = 0.2473). Attitudes of food establishment owners and managers towards feral pigeons are most influenced by how often they see feral pigeons (∆AICc ≤ W = 0:465). Therefore, direct experience rather than knowledge, engagement, action or socio-demographics has the most influence on attitudes of owners and managers of food establishments. These results suggest that feral pigeon populations are largely dependent on the availability of anthropogenic foods. Reducing the food provided by people may limit feral pigeon populations. Reductions in pigeon populations are also likely to change attitudes of business owners and reduce conflict because they will be less likely to encounter pigeons. Limiting feeding and access to food waste is probably the most effective way of managing pigeon populations.</p>

Pigeons and People: Resource Ecology and Human Dimensions of Urban Wildlife

<p>Urban areas and human populations are growing. Cities provide highly modified habitat for species that can adapt their feeding and other behaviours. The growth of urban landscapes and human populations may result in an increase in human-wildlife conflict. Businesses which prepare and sell food (food establishments) may be more likely to encounter conflict with urban wildlife, which may lead to negative attitudes towards urban wildlife. Negative attitudes towards wildlife could create polarised communities and possibly affect the success of environmental initiatives. This study sought to understand (1) how feral pigeons use urban environments and the resources key to their distribution and congregation; (2) whether feral pigeons are food limited in Wellington City; and (3) how the interactions of owners and managers of food establishments with feral pigeons influence their attitudes to feral pigeons. I used 8 transects through the central City which covered a representative sample of urban habitats, including the central business district, green space, and waterfront to estimate resource selection. Bird capture and banding were used to determine feral pigeon condition at a range of sites across the City and included a mix of high, medium and low anthropogenic fed sites. A written survey of owners and managers of food establishments in Wellington was conducted to evaluate attitudes to feral pigeons (n = 62). Feral pigeon resource selection is mainly influenced by people and where they choose to eat (∆AIC ≤ W = 0.999), such as sites with outdoor seating where people may directly feed feral pigeons. However, once a site has been selected, areas with tertiary vegetation and disposed food (W = 0.324 and W = 0.297) are the most likely to attract larger flocks of feral pigeons (although a number of other variables also influence flock size, such as availability of freshwater). Feral pigeons do not appear to be food limited in Wellington as condition was not significantly different between sites (n=48, body condition, (body mass/tarsus length) Kruskal-Wallis = 2.06, p = 0.36; keel condition, Kruskal-Wallis = 0.7283, p = 0.6948; feather condition Kruskal-Wallis = 2.7943, p = 0.2473). Attitudes of food establishment owners and managers towards feral pigeons are most influenced by how often they see feral pigeons (∆AICc ≤ W = 0:465). Therefore, direct experience rather than knowledge, engagement, action or socio-demographics has the most influence on attitudes of owners and managers of food establishments. These results suggest that feral pigeon populations are largely dependent on the availability of anthropogenic foods. Reducing the food provided by people may limit feral pigeon populations. Reductions in pigeon populations are also likely to change attitudes of business owners and reduce conflict because they will be less likely to encounter pigeons. Limiting feeding and access to food waste is probably the most effective way of managing pigeon populations.</p>

Public health risk of Giardia and Cryptosporidium infection from urban wildlife

Introduction/Objective The dedication of large areas in Anchorage for urban park environments encouraged increased interaction between migrating Canadian geese, approximately 1500 moose and humans. Urban wildlife inhabiting public establishments such as parking lots, creeks, playgrounds, and suburban backyards results in significant environmental fecal contamination. Previous studies found varying potential for zoonotic transmission of parasites through direct fecal contact or water contamination. With a significant number of vulnerable unsheltered individuals in Anchorage using public green belts, there is risk of asymptomatic, under diagnosed, parasitic infection due to exposure to fecal contamination. Methods/Case Report To assess environmental risk of parasitic zoonosis from urban wildlife, we surveyed fourteen high impact exposure areas including waterways and green spaces throughout Anchorage with ground collection of 106 fecal samples from moose (n=76) and geese (n=30). Using standard rapid antigen immunoassay detection method, we identified preserved samples for the presence of Cryptosporidium or Giardia. Results (if a Case Study enter NA) Overall evaluation of fecal samples found 23.6% positive for parasitic colonization in 87% of collection sites. Giardia was identified in 10.4% and Cryptosporidium in 13.2%. Comparative evaluation of each species found geese with a higher percentage of Cryptosporidium isolated (30%) when compared to moose (6%). Giardia was predominately found in moose (13%) with very few geese colonized. Conclusion Considering the high population of outdoor enthusiasts and a significant number of unsheltered individuals in Anchorage, discovering nearly a quarter of urban moose and geese colonized with Giardia and/or Cryptosporidium identifies a significant public health risk. Often using creeks and lagoons as their water source Anchorage’s vulnerable population includes a large number of immunocompromised HIV positive individuals in which parasitic infection, especially Cryptosporidium, can cause overall poor health and reduced quality of life. Therefore, active surveillance of parasitic colonization in urban wildlife is essential and continued evaluation necessary to determine public health risk.

Public risk from antibiotic resistant Escherichia coli colonized in urban wildlife

Introduction/Objective Escherichia coli is an especially dangerous health threat to humans when it develops multi- drug resistance. Decreased sensitivity to quinolone and beta-lactam antibiotics has been documented with antibiotic use in health care and agriculture. Migrating birds interact with wastewater treatment plants colonized with drug resistant Escherichia coli potentially transfer to other wildlife. Anchorage is home to 1500 moose and migrating geese providing a potential risk for Escherichia coli colonization. Because fecal contamination is abundant in Anchorage’s greenspaces, we evaluated the presence of Escherichia coli and antibiotic resistance in urban moose. Methods/Case Report Between 2018-2020, moose fecal samples were ground collected during the spring thaw within Anchorage Alaska, placed in transport media and cultured to MacConkey agar. Lactose fermenting colonies were further identified as Escherichia coli by biochemical testing and sub-cultured to sheep blood agar for antimicrobial evaluation. Using the Kirby Bauer method, antimicrobial sensitivity for cefpodoxime, ampicillin, piperacillin/tazobactam, gentamycin and ciprofloxacin were performed and interpreted using Clinical Laboratory Standards Institute guidelines Results (if a Case Study enter NA) A total of 150 samples were analyzed with 39% positive for Escherichia coli: fourteen in 2018, seventeen in 2019, and twenty-eight in 2020. In 2018, resistance was significant in ampicillin (43%), limited in piperacillin/tazobactam and cefpodoxime (14%). In 2019 found a 40% overall increase of antimicrobial resistance and by 2020 Escherichia coli isolates were resistant in all antibiotics tested: cefpodoxime (100%), ampicillin (96%), piperacillin/tazobactam (43%), gentamycin (35%), and ciprofloxacin (32%). Conclusion Measuring resistant patterns of Escherichia coli in wildlife is essential to understand the risk of colonization. Transmission of infectious agents can occur due to environmental exposure and Anchorage has expanded greenspaces resulting in increased human interaction with urban wildlife. In 2018, we found antimicrobial resistance only to ampicillin but subsequent years discovered decrease sensitivity and by 2020 Escherichia coli isolates resistant to one or more antibiotics including beta-lactams, quinolones, and aminoglycosides. The risk of zoonosis of multi-drug resistant Escherichia coli, especially in our housing insecure, has a significant impact for patient outcome.