ASSESS THE EFFECTIVENESS OF STRUCTURED TEACHING PROGRAMMED REGARDING COLLECTION OF CORD BLOOD FOR STEM CELL THERAPY AT BRAM HOSPITAL OF RAIPUR

The stem cells, derived from the cord blood are hematopoietic stem cells. These have immense potential in curing blood related disorders like blood cancers, thalassemia etc. These can be useful in treating tissue related disorders of heart, bone, spinal cord etc. Stem cells are characterized by the ability to renew through mitotic cell division and differentiate into a diverse range of specialized cell types. Stem Cells are the basic building blocks of the body and have the potential to replenish other cells and give rise to number of tissues which constitute different organs. To conduct the study, 60 antenatal mothers are selected the age range of subjects was 21 to 40 years. The need for the study arises after knowing those antenatal mothers were unaware and having inadequate knowledge regarding cord blood collection for stem cell therapy. The above facts created an interest to conduct a structured teaching programme to assess its effectiveness on knowledge and attitude of antenatal mother on cord blood collection for stem cell therapy. The pretest, the mean score of knowledge is 13.2 mean % is 50.7, & attitude is 31.58, mean % 63.16, the post test the mean score of knowledge is 18.1, mean % is 69.65. Attitude 38.05, mean % 76.1.

Evaluation of Microbial Contamination Rates in Umbilical Cord Blood Collection and Processing for Biobanking in Vietnam

The umbilical cord blood (CB) has recently been considered an abundant source of hematopoietic stem cells (HSCs) for transplantation compared to bone marrow. However, the collection and processing of CB have a high risk of microbial contamination. Hence, the procedures to collect and process the UCB are carefully considered. This study evaluates the microbial contamination rate to find the frequency and distribution of bacterial organisms among CB sampling and processing in Vietnam. In addition, this study correlates with contamination rates between the delivery method, cesarean section, and vaginal delivery. The results create best practices to avoid a high level of contamination of UCB during collection and processing for biobanking.

Spinal cord injury as an indicator of abuse in forensic assessment of abusive head trauma (AHT)

Abusive head trauma (AHT) in children is notoriously one of the most challenging diagnoses for the forensic pathologist. The pathological “triad”, a combination of intracranial subdural haematoma, cerebral oedema with hypoxic-ischaemic changes and retinal haemorrhages, is frequently argued to be insufficient to support a corroborated verdict of abuse. Data from all available English-language scientific literature involving radiological and neuropathological spinal cord examination is reviewed here in order to assess the contribution of spinal cord changes in differentiating abusive from accidental head trauma. In agreement with the statistically proven association between spinal subdural haemorrhage (SDH) and abuse (Choudhary et al. in Radiology 262:216–223, 2012), spinal blood collection proved to be the most indicative finding related to abusive aetiology. The incidence of spinal blood collection is as much as 44–48% when all the spinal cord levels are analysed as opposed to just 0–18% when the assessment is performed at cervical level only, in agreement with the evidence of the most frequent spinal SDH location at thoracolumbar rather than cervical level. In this review, the source of spinal cord blood collection and how the age of the child relates to the position of spinal cord lesions is also discussed. We concluded that the ante mortem MRI examination and post mortem examination of whole-length spinal cord is of fundamental interest for the assessment of abuse in the forensic setting.

Emotional labour and cord blood collection: frontline perspectives

PurposeThis paper aims to examine emotional labour in the work of frontline staff (FLS) of the Canadian Blood Services' Cord Blood Bank (CBB), contributes to understandings of emotional labour by allied healthcare workers and suggests implications for healthcare managers.Design/methodology/approachQualitative interviews with 15 FLS were conducted and analyzed as part of a process evaluation of donor recruitment and cord blood collection in Canada.FindingsEmotional labour with donors and hospital staff emerged as a vital component of FLS' donor recruitment and cord blood collection work. Emotional labour was performed with donors to contribute to a positive birthing experience, facilitate communication and provide support. Emotional labour was performed with hospital staff to gain acceptance and build relationships, enlist support and navigate hierarchies of authority.Research limitations/implicationsThe results indicate that FLS perform emotional labour with women to provide donor care and with hospital staff to facilitate organizational conditions. The findings are based on FLS' accounts of their work and would be enhanced by research that examines the perspectives of donors and hospital staff.Practical implicationsAttention should be paid to organizational conditions that induce the performance of emotional labour and may add to FLS workload. Formal reciprocal arrangements between FLS and hospital staff may reduce the responsibility on FLS and enable them to focus on recruitment and collections.Originality/valueThis paper addresses a gap in the healthcare management literature by identifying the emotional labour of allied healthcare workers. It also contributes to the cord blood banking literature by providing empirically grounded analysis of frontline collection staff.

Adapting Cord Blood Collection and Banking Standard Operating Procedures for HLA-Homozygous Induced Pluripotent Stem Cells Production and Banking for Clinical Application

In this article, we will discuss the main aspects to be considered to define standard operation procedures (SOPs) for the creation of an induced pluripotent stem cell (iPSC) bank using cord blood (CB)—or similar cell type—bank guidelines for clinical aims. To do this, we adapt the pre-existing SOP for CB banking that can be complementary for iPSCs. Some aspects of iPSC manufacturing and the particular nature of these cells call for special attention, such as the potential multiple applications of the cells, proper explanation to the donor for consent of use, the genomic stability and the risk of genetic privacy disclosure. Some aspects of the iPSC SOP are solidly established by CB banking procedures, other procedures have good consensus in the scientific and medical community, while others still need to be further debated and settled. Given the international sharing vocation of iPSC banking, there is an urgent need by scientists, clinicians and regulators internationally to harmonize standards and allow future sample interchange between many iPSC bank initiatives that are springing up worldwide.

Cord Blood Banking: Antenatal Care Provider’s Roles and Responsibilities

Background. Umbilical Cord Blood (UCB) banking done either for private storage or for donation to public cord blood banks involves active participation of obstetricians. Counseling the expectant parents, providing them with unbiased and balanced information, and collecting the UCB with diligence confer a lot of social as well as moral responsibility upon obstetricians. This makes it even more important that the obstetricians in current practice stay well-informed and updated with UCB collection and its storage guidelines. The present study was conducted to assess the current status of obstetricians about UCB banking in terms of their awareness, attitude, and expectations from it. Materials and Methods. A cross-sectional study was conducted across three hospitals. A self-administered 22-item questionnaire was given to obstetricians to assess their awareness, attitude, and expectations about UCB banking. Finally, 154 completed questionnaires were analyzed using SPSS software (version 15.0). The awareness, attitude, and expectations were assessed and reported as primary endpoints and the self-rated knowledge levels, and sources of information were reported as secondary endpoints. Results. Overall, the awareness was poor, but the attitude was favorable for UCB banking amongst obstetricians. Around 74% felt that obstetricians must be well-informed about UCB banking-related counseling and collection protocols. However, 55% felt it to be an additional burden for the obstetrician, and 57% believed that financial compensation must be given to obstetricians involved with cord blood collection procedures. The majority remained unclear about their expectations from UCB banking. The self-rated knowledge was poor and very poor for 75% obstetricians. 89.6% derived their information from representatives of private cord blood companies. Conclusion. Although poor in awareness levels, obstetricians possessed a favorable attitude towards UCB banking. Continuing medical education needs to focus more on such current issues of public importance to keep professionals updated. This is one way to minimise percolation of wrong facts and figures by the industries with conflicting interest to the healthcare providers.