cell autonomy
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2021 ◽  
Author(s):  
Lusheng Fan ◽  
Cui Zhang ◽  
Yong Zhang ◽  
Ethan Stewart ◽  
Jakub Jez ◽  
...  

Mobile microRNAs (miRNAs) serve as local and long-distance signals in developmental patterning and stress responses in plants. However, mechanisms governing the non-cell autonomous activities of miRNAs remain elusive. Here, we show that mutations that disrupt microtubule dynamics are specifically defective for the non-cell autonomous actions of mobile miRNAs, including miR165/6 that is produced in the endodermis and moves to the vasculature to pattern xylem cell fates in Arabidopsis roots. We show that KTN1, a subunit of a microtubule-severing enzyme, is required in source and intermediary cells to inhibit the loading of miR165/6 into ARGONUATE1 (AGO1), which is cell-autonomous, to enable the miRNA's cell exit. Microtubule disruption enhances the association of miR165/6 with AGO1 in the cytosol. These findings suggest that, while cell-autonomous miRNAs load into AGO1 in the nucleus, cytoplasmic AGO1 loading of mobile miRNAs is a key step regulated by microtubules to promote the range of miRNA's cell-to-cell movement.


2018 ◽  
Vol 35 (2) ◽  
pp. 189-190
Author(s):  
David F. Albertini
Keyword(s):  

2017 ◽  
Author(s):  
Catalina Casillas ◽  
Henk Roelink

AbstractSonic Hedgehog (Shh) signaling is characterized by strict non-cell autonomy; cells expressing Shh do not respond to their ligand. Here, we identify several Shh mutations that gain the ability to activate the Hedgehog (Hh) pathway in cis. This activation requires the extracellular cysteine rich domain of Smoothened, but is otherwise independent of Ptch1/2. Many of the identified mutations disrupt either a highly conserved catalytic motif found in peptidases or an a-helix domain frequently mutated in holoprosencephaly-causing SHH alleles. The expression of gain-of-function mutants often results in the accumulation of unprocessed Shh pro-peptide, a form of Shh we demonstrate is sufficient to activate the Hh response cell-autonomously. Our results demonstrate that Shh is capable of activating the Hh pathway via Smo independently of Ptch1/2, and that it harbors an intrinsic mechanism that prevents cell-autonomous activation of the pathway to favor non-cell autonomous signaling.


eNeuro ◽  
2017 ◽  
Vol 4 (4) ◽  
pp. ENEURO.0160-17.2017 ◽  
Author(s):  
Takako Noguchi ◽  
Tanya L. Leise ◽  
Nathaniel J. Kingsbury ◽  
Tanja Diemer ◽  
Lexie L. Wang ◽  
...  

2014 ◽  
Vol 16 (5) ◽  
pp. 055003 ◽  
Author(s):  
Holley E Lynch ◽  
Jim Veldhuis ◽  
G Wayne Brodland ◽  
M Shane Hutson

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