Hematological Changes Following Oral Administration of Aqueous Leaf Extract of Ficus capensis in Albino Rats

2016 ◽  
Vol 5 (1) ◽  
pp. 1-7
Author(s):  
N Njoku-Oji ◽  
C Nwike ◽  
U Dimkpa ◽  
N Ifegwu ◽  
L Anike ◽  
...  
Keyword(s):  
Oral Administration ◽  
Leaf Extract ◽  
Albino Rats ◽  
Aqueous Leaf Extract ◽  
Hematological Changes

scholarly journals In vitro Antioxidant and In vivo Hepatocurative and Nephrocurative Activities of Aqueous Leaf Extract of Newbouldia laevis in Albino Rats

2021 ◽  
pp. 114-125
Author(s):  
Mohammed A. Sulaiman ◽  
Mahmoud S. Jada ◽  
Augustine Elizabeth ◽  
Abubakar Umar Modibbo
Keyword(s):  
Oral Administration ◽  
Leaf Extract ◽  
Kidney Diseases ◽  
Albino Rats ◽  
Protein Levels ◽  
Ameliorative Effect ◽  
Liver And Kidney ◽  
Aqueous Leaf Extract

The in vitro antioxidant activity and in vivo hepatocurative and nephrocurative potential of Newbouldia laevis aqueous leaf extract (NLALE) was evaluated. The study used 30 male, albino rats (Rattus norvegicus) weighing 180 ± 20 g, of which 25 were intoxicated by oral administration of a single dose of diclofenac (100 mg/kg b. wt.). Animals were treated by oral administration of silymarin (200 mg/kg b. wt.), furosemide (1.5 mg/kg b. wt.) and NLALE (200 mg/kg and 400 mg/kg b. wt.) for seven consecutive days before animals were sacrificed on the 8th day and serum/plasma was analyzed for biochemical markers of hepatotoxicity and nephrotoxicity. Phytochemical screening of NLALE revealed the presence of alkaloids, flavonoids, glycosides, phenols, saponins, steroids and tannins. The extract scavenged DPPH radical, reduced Fe3+ and inhibited TBARs in comparable manner to ascorbic acid in vitro. NLALE also attenuated diclofenac-induced liver and kidney intoxication as indicated by the significantly (p<0.05) reduced levels of serum biomarkers of hepatotoxicity: ALT, AST, bilirubin, but increased total protein levels and nephrotoxicity: urea, creatinine, Na+ and K+. The observed effects are dose dependent as the 400 mg/kg b. wt. appeared to be more potent than the 200 mg/kg b. wt. dose. It may be concluded from this study that Newbouldia laevis leaf has ameliorative effect against diclofenac-induced hepatotoxicity and nephrotoxicity probably through antioxidative mechanism and the curative claim and the folkloric use of the plant in the treatment of liver and kidney diseases have been scientifically validated

scholarly journals HEPATOPROTECTIVE AND ANTI-HEPATOTOXIC ACTIVITIES OF AQUEOUS LEAF EXTRACT OF TACAZZEA BARTERI AGAINST CARBON TETRACHLORIDE INDUCED HEPATOTOXICITY IN ALBINO RATS

2013 ◽  
Vol 2 (9) ◽  
pp. 60-65 ◽  
Author(s):  
Obioha Mary Quinette Uru ◽  
Ilodigwe Emmanuel Emeka ◽  
Ajaghaku Daniel Lotanna ◽  
Umeokoli Blessing Ogechukwu
Keyword(s):  
Carbon Tetrachloride ◽  
Leaf Extract ◽  
Albino Rats ◽  
Aqueous Leaf Extract

Acute and sub-acute toxicity of antisickling polyherbal extract in Wistar albino rats

2020 ◽  
Vol 23 ◽  
pp. 41-53
Author(s):  
O.O. Amujoyegbe ◽  
M. Idu ◽  
J.M. Agbedahunsi ◽  
E.M. Obuotor ◽  
I.J. Olawuni ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Blood Cell ◽  
Oral Administration ◽  
Sickle Cell ◽  
Leaf Extract ◽  
Hematological Parameter ◽  
Albino Rats ◽  
Histopathological Evaluation ◽  
High Doses ◽  
Cell Disorder

Piper guineense, Gongronema latifolium and Cymbopogon citratus (PGC) serve as an effective polyherbal antisickling extract used in the  management of sickle cell disorder. This present study assessed the toxicity effect of the ethanol leaf extract of PGC in rats. The acute oral toxicity  test of the polyherbal was evaluated in albino rats using a single-dose based on behavioral changes and mortality. Sub-acute toxicological  evaluation of PGC was examined using biochemical, hematological and histopathological methods. Biochemical analysis was carried out using liver markers enzymes, kidney markers enzymes, and lipid profiles. The hematological measurement includes white blood cell counts (WBC),  Lymphocytes (LYM), monocytes (MON), granulocytes (GRAN), Red blood cell count (RBC). The organs (liver, kidney, and heart) were collected and prepared using standard protocols with hematoxylin and eosin for histopathological evaluation. The acute toxicity study of ethanol leaf extract of PGC up to the limit dose of 2000 mg/kg body weight of the animals used did not produce any signs and symptoms of toxic effects or mortality after 48 hrs. of oral administration. There were no significant differences (p < 0.001) in the observed values between the control and the treated groups for all the biochemical and hematological parameters analyzed. Histopathological evaluation of the organs demonstrated mild degeneration in the kidney and liver while the heart revealed no pathological changes in the treated group of rats. The result of acute toxicity indicates that the  combined antisickling polyherbal PGC extract appeared to be safe and non- toxic. Our findings for the 28 days daily oral administration of PGC extract was dose dependent and well tolerated by the animals. Although, slight changes were observed in some biochemical parameters and histology of the kidney and liver at high doses when compared with control rats. Therefore, the consumption of the antisickling polyherbal PGC extract orally should be used encouraged at lower doses and high doses should be avoided for the management of sickle cell disorder until subjected to further cytotoxicity evaluation. Keywords: Polyherbal combination, acute toxicity, subacute toxicity, biochemical parameter, hematological parameter, histopathological parameters.

scholarly journals Gastroprotective effect of the aqueous leaf extract of Guiera senegalensis in Albino rats

2013 ◽  
Vol 6 (10) ◽  
pp. 771-775 ◽  
Author(s):  
GC Akuodor ◽  
AD Essien ◽  
E David-Oku ◽  
KC Chilaka ◽  
JL Akpan ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Albino Rats ◽  
Gastroprotective Effect ◽  
Aqueous Leaf Extract ◽  
Guiera Senegalensis
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