Changes in Erythrocytic Membrane Free Energy of Albino Rabbits Administered Ethanol Leaf Extract of Spondias mombin Linn

2012 ◽  
Vol 2 (3) ◽  
pp. 199-208
Author(s):  
C. Igwe
Keyword(s):  
Free Energy ◽  
Leaf Extract ◽  
Spondias Mombin

scholarly journals IN VITRO AND IN SILICO EVALUATION OF XANTHINE OXIDASE INHIBITORY ACTIVITY OF QUERCETIN CONTAINED IN SONCHUS ARVENSIS LEAF EXTRACT

2017 ◽  
Vol 10 (14) ◽  
pp. 50
Author(s):  
Rini Hendriani ◽  
Nursamsiar Nursamsiar ◽  
Ami Tjitraresmi
Keyword(s):  
Free Energy ◽  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
In Silico ◽  
Leaf Extract ◽  
Binding Free Energy ◽  
Uv Spectrophotometry ◽  
Docking Simulations ◽  
Oxidase Enzyme

Objective: The aim of the present study was to examine the inhibiting effects of quercetin contained in Sonchusarvensis leaf extract on the activity of xanthine oxidase, an essential enzyme for uric acid synthesis.Methods: Activity test was conducted in vitro by measuring the activity of xanthine oxidase using UV spectrophotometry and in silico by determining the interaction of quercetin and allopurinol (as comparation drug) with xanthine oxidase enzyme in terms of hydrogen bonds and binding free energy. Docking simulations were performed by Autodock4.2 package.Results: The active fraction, using the solvent n-hexane, ethyl acetate and water, tested the inhibitory activity of the xanthine oxidase enzyme in vitro obtained respectively IC50 of 263.19, 16.20 and 141.80 μg/ml. Isolates with highest activity identified as quercetin. The xanthine oxidase enzyme inhibitory activity insilico by molecular docking showed quercetin has free energy binding ˗7.71 kcal/mol, more negative than that of allopurinol ˗5.63 kcal/mol.Conclusion: This shows the affinity of quercetin stronger than that of allopurinol; so that it can be predicted that quercetin was more potential to inhibit xanthine oxidase enzyme activity. Thus the extract of the S. arvensis leaves containing the active compound quercetin was a potential use as antihyperuricemia.  

scholarly journals Toxicological evaluation of aqueous leaf extract of Spondias mombin using albino rat

2018 ◽  
pp. 23-30
Author(s):  
NWAOGWUGWU JOEL, FRIDAY UHEGBU ◽  
OKEREKE STANLEY, EGEGE AMAKA, ATASI OKECHUKWU
Keyword(s):  
Leaf Extract ◽  
Lethal Dose ◽  
Albino Rats ◽  
Albino Rat ◽  
Toxicological Evaluation ◽  
Spondias Mombin ◽  
Reduced Body ◽  
Aqueous Leaf Extract ◽  
Dose Dependent ◽  
Different Doses

Toxicological evaluation of aqueous leaf extract of Spondias mombin using albino rat was carried out. Twenty-five albino rats were grouped into five groups of five rats each. Group 1 served as the control, while groups 2-5 served as the test groups. The test groups were placed on different doses of the studied leaf extract after the lethal dose was determined. The studied extractconsists mainly of flavonoids, saponins, alkaloid, tannins and cyanogenic glycosides, which have considerable therapeutic values, and reduced body weight considerably in test rats against the control. Levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP)in test rats reduced significantly (p<0.05) when compared to the control. However, the observed histopathological changes associated with the kidney and liver of test rats against those of the control may suggest that the toxicity of the extract could be dose dependent. This study has shown the toxicological evaluation of aqueous leaf extract of S. mombin using albino rat.

EFFECT OF PRE-COPULATORY TREATMENT WITH AQUEOUS LEAF EXTRACT OF Spondias mombin ON GESTATIONAL CHARACSTERISTICS OF RABBITS

2019 ◽  
Vol 16 (2) ◽  
pp. 83-87
Author(s):  
A. A. OLOYE ◽  
N. OBI
Keyword(s):  
Birth Weight ◽  
Litter Size ◽  
Leaf Extract ◽  
Control Group ◽  
Gestation Length ◽  
Spondias Mombin ◽  
Average Litter Size ◽  
Aqueous Leaf Extract ◽  
And Control

Fifteen plurigravida rabbits weighing between 2.0 and 2.6 kg were used for the study of gestational characteristics after pre-copulatory oral administration of aqueous leaf extract of Spondias mombin. The fifteen does were divided into three groups of five does per group. Four untreated reproductively proven bucks kept separately in different cages were used for copulation. Dosages administered were 400mg/kg and 800mg/kg for groups A and B respectively while distilled water was served to control group C. Mating, pregnancy diagnosis using ultrasound and determination of birth parameters were conducted subsequently at the end of extract treatment. Average litter size,birth weight and gestation length observed in all dosage groups had statistically similar values (p≥0.05).However, it is worth noting the relatively higher values of average litter size in 800mg/kg dosage group and control (5.60±0.40) compared to 400mg/kg group (5.40±0.75) and the highest birth weight value recorded for 800mg/kg (46.63±3.55) compared to 400mg\kg group (45.30±3.22) and control (42.38±3.74). It was concluded that pre-copulatory administration of aqueous leaf extract of Spondias mombin at dosages as high as 800mg/kg to rabbit does not have deleterious effect on its prolificacy and does have pro-fertility tendencies.        

scholarly journals Spermiogram and testicular morphological studies of the buck after treatment with ethanol leaf extract of Spondias mombin

2017 ◽  
Vol 15 (1) ◽  
pp. 72 ◽  
Author(s):  
A.A. Oloye ◽  
O.E. Ola-Davies ◽  
O.L. Ajayi ◽  
M.O. Oyeyemi ◽  
S.A. Olurode ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Spondias Mombin ◽  
Morphological Studies
Sign in / Sign up

Export Citation Format

Share Document