Indian Lettuce Extract as Feed Additive Enhances Immunological Parameters in Mono-Sex Nile Tilapia against Aeromonas hydrophila

2015 ◽  
Vol 5 (5) ◽  
pp. 309-318 ◽  
Author(s):  
Farzana Yeasmin ◽  
Md. Hossain ◽  
Aisha Khatun ◽  
Mohammad Rahman ◽  
Md. Alam
2016 ◽  
Vol 48 (6) ◽  
pp. 3163-3173 ◽  
Author(s):  
Miao Wang ◽  
Guanbin Liu ◽  
Maixin Lu ◽  
Xiaoli Ke ◽  
Zhigang Liu ◽  
...  

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Preetham Elumalai ◽  
Amitha Kurian ◽  
Sreeja Lakshmi ◽  
Mohamed Saiyad Musthafa ◽  
Einar Ringo ◽  
...  

The present study addressed the effects of Leucas aspera enriched diet in Nile tilapia. Three hundred Nile tilapia were fed Leucas aspera as follows: 0 g kg-1 L. aspera (C-control), 5 g kg-1 L. aspera (T1), 10 g kg-1 L. aspera (T2) and 15 g kg-1 L. aspera (T3). After 30 days of feeding, significant (P<0.05) increase in growth performance was noticed by feeding the fish the T2 diet. Thereafter fish were intraperitoneal injected with Aeromonas hydrophila in challenge test. After 21 days of challenge, highest survival rate (70%) was observed in fish fed the T3 diet followed by fish fed T2 diet (65%). Serum immunological parameters such as phagocytosis, alternative complement activity, respiratory burst activity and lysozyme activity were significantly (P<0.05) enhanced in fish fed all inclusion levels of L. aspera with the maximum activity in fish fed the T2 diet. Hematological parameters were significantly (P<0.05) higher in all groups fed L. aspera diets vs. control fed fish. No histopathological changes in liver were observed in fish fed the T2 diet in the histology study. Gene expression study revealed the upregulation in the expression of COX-2 and GR genes. In conclusion, the current results suggest that dietary administration of L. aspera especially the T2 diet, has beneficial effects in improving immunity and can mitigate the adverse effects of A. hydrophila infection in Nile tilapia.


2021 ◽  
Vol 12 ◽  
Author(s):  
Suelen Aparecida Suphoronski ◽  
Felipe Pinheiro de Souza ◽  
Roberta Torres Chideroli ◽  
Leonardo Mantovani Favero ◽  
Natália Amoroso Ferrari ◽  
...  

In the present study, we evaluated the effects of administering Enterococcus faecium in food and/or water on the hematological and immunological parameters, intestinal microbiota, resistance to bacterial diseases (streptococcosis and francisellosis) and growth of Nile tilapia. Before the in vivo experiment, probiotic bacteria isolated from Nile tilapia were selected via inhibition tests. Sequencing, annotation, and assembly of the complete genome of the selected bacteria as well as other tests were performed using bioinformatics tools. Three treatments were implemented: G1 (probiotic feeding), G2 (probiotic in water), and G3 (probiotic in food and water); and a negative control (NC) was also employed. Treatment lasted 38 days, and each group consisted of fish and two repetitions. The fish were divided and infected with Streptococcus agalactiae S13 (serotype Ib) and Francisella orientalis. The G1 group had a higher average final weight gain than the G2, G3, and NC groups. Further, a significant increase in the number of thrombocytes was observed in the groups administered probiotics in the diet (G1 and G3). A statistical difference was observed in the mortality of fish infected with S. agalactiae in the NC compared to the treated groups. Cetobacterium was the 43 most abundant genus in the intestinal microbiota of all groups, including the NC group. E. faecium increased the immunity of fish administered the treatment and decreased the mortality caused by S. agalactiae. As an autochtone probiotic, E. faecium does not interfere with the local ecosystem and thus has a great probiotic potential for Nile tilapia in Brazil.


Author(s):  
Arne A Ratulangi ◽  
Reiny Tumbol ◽  
Hengky Manoppo ◽  
Henneke Pangkey

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.


Aquaculture ◽  
2021 ◽  
Vol 536 ◽  
pp. 736497
Author(s):  
Moisés Angel Poli ◽  
Mateus Aranha Martins ◽  
Scheila Anelise Pereira ◽  
Gabriel Fernandes Alves Jesus ◽  
Maurício Laterça Martins ◽  
...  

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