Effect ofBacillus cereusas a water or feed additive on the gut microbiota and immunological parameters of Nile tilapia

2016 ◽  
Vol 48 (6) ◽  
pp. 3163-3173 ◽  
Author(s):  
Miao Wang ◽  
Guanbin Liu ◽  
Maixin Lu ◽  
Xiaoli Ke ◽  
Zhigang Liu ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Nile Tilapia ◽  
Feed Additive ◽  
Immunological Parameters

scholarly journals Effect of Enterococcus faecium as a Water and/or Feed Additive on the Gut Microbiota, Hematologic and Immunological Parameters, and Resistance Against Francisellosis and Streptococcosis in Nile Tilapia (Oreochromis niloticus)

2021 ◽  
Vol 12 ◽  
Author(s):  
Suelen Aparecida Suphoronski ◽  
Felipe Pinheiro de Souza ◽  
Roberta Torres Chideroli ◽  
Leonardo Mantovani Favero ◽  
Natália Amoroso Ferrari ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Enterococcus Faecium ◽  
Nile Tilapia ◽  
Negative Control ◽  
Feed Additive ◽  
Immunological Parameters ◽  
Probiotic Potential ◽  
Local Ecosystem ◽  
Abundant Genus

In the present study, we evaluated the effects of administering Enterococcus faecium in food and/or water on the hematological and immunological parameters, intestinal microbiota, resistance to bacterial diseases (streptococcosis and francisellosis) and growth of Nile tilapia. Before the in vivo experiment, probiotic bacteria isolated from Nile tilapia were selected via inhibition tests. Sequencing, annotation, and assembly of the complete genome of the selected bacteria as well as other tests were performed using bioinformatics tools. Three treatments were implemented: G1 (probiotic feeding), G2 (probiotic in water), and G3 (probiotic in food and water); and a negative control (NC) was also employed. Treatment lasted 38 days, and each group consisted of fish and two repetitions. The fish were divided and infected with Streptococcus agalactiae S13 (serotype Ib) and Francisella orientalis. The G1 group had a higher average final weight gain than the G2, G3, and NC groups. Further, a significant increase in the number of thrombocytes was observed in the groups administered probiotics in the diet (G1 and G3). A statistical difference was observed in the mortality of fish infected with S. agalactiae in the NC compared to the treated groups. Cetobacterium was the 43 most abundant genus in the intestinal microbiota of all groups, including the NC group. E. faecium increased the immunity of fish administered the treatment and decreased the mortality caused by S. agalactiae. As an autochtone probiotic, E. faecium does not interfere with the local ecosystem and thus has a great probiotic potential for Nile tilapia in Brazil.

Increasing stocking densities affect hemato-immunological parameters of Nile tilapia reared in an integrated system with Pacific white shrimp using biofloc technology

Aquaculture ◽  
2021 ◽  
Vol 536 ◽  
pp. 736497
Author(s):  
Moisés Angel Poli ◽  
Mateus Aranha Martins ◽  
Scheila Anelise Pereira ◽  
Gabriel Fernandes Alves Jesus ◽  
Maurício Laterça Martins ◽  
...  
Keyword(s):  
Nile Tilapia ◽  
Pacific White Shrimp ◽  
Integrated System ◽  
White Shrimp ◽  
Immunological Parameters ◽  
Biofloc Technology

Lactuca indica extract as feed additive enhances immunological parameters and disease resistance in Epinephelus bruneus to Streptococcus iniae

Aquaculture ◽  
2011 ◽  
Vol 318 (1-2) ◽  
pp. 43-47 ◽  
Author(s):  
Ramasamy Harikrishnan ◽  
Ju-Sang Kim ◽  
Man-Chul Kim ◽  
Chellam Balasundaram ◽  
Moon-Soo Heo
Keyword(s):  
Disease Resistance ◽  
Feed Additive ◽  
Streptococcus Iniae ◽  
Immunological Parameters ◽  
Lactuca Indica ◽  
Epinephelus Bruneus

scholarly journals The Impact of Sampling Season and Catching Site (Wild and Aquaculture) on Gut Microbiota Composition and Diversity of Nile Tilapia (Oreochromis niloticus)

Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 180
Author(s):  
Negash Kabtimer Bereded ◽  
Getachew Beneberu Abebe ◽  
Solomon Workneh Fanta ◽  
Manuel Curto ◽  
Herwig Waidbacher ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Nile Tilapia ◽  
Alpha Diversity ◽  
Environmental Parameters ◽  
Illumina Miseq ◽  
Microbial Composition ◽  
Lake Tana ◽  
Future Work ◽  
The Impact ◽  
Sampling Season

The gut microbiota of fishes is known to play an essential role in diverse aspects of host biology. The gut microbiota of fish is affected by various environmental parameters, including temperature changes, salinity and diet. Studies of effect of environment on gut microbiota enables to have a further understanding of what comprises a healthy microbiota under different environmental conditions. However, there is insufficient understanding regarding the effects of sampling season and catching site (wild and aquaculture) on the gut microbiota of Nile tilapia. This study characterised gut microbial composition and diversity from samples collected from Lake Tana and the Bahir Dar aquaculture facility centre using 16S rDNA Illumina MiSeq platform sequencing. Firmicutes and Fusobacteria were the most dominant phyla in the Lake Tana samples, while Proteobacteria was the most dominant in the aquaculture samples. The results of differential abundance testing clearly indicated significant differences for Firmicutes, Fusobacteria, Bacteroidetes and Cyanobacteria across sampling months. However, Proteobacteria, Chloroflexi, Fusobacteria and Cyanobacteria were significantly enriched in the comparison of samples from the Lake Tana and aquaculture centre. Significant differences were observed in microbial diversity across sampling months and between wild and captive Nile tilapia. The alpha diversity clearly showed that samples from the aquaculture centre (captive) had a higher diversity than the wild Nile tilapia samples from Lake Tana. The core gut microbiota of all samples of Nile tilapia used in our study comprised Firmicutes, Proteobacteria and Fusobacteria. This study clearly showed the impact of sampling season and catching site (wild and aquaculture) on the diversity and composition of bacterial communities associated with the gut of Nile tilapia. Overall, this is the first study on the effects of sampling season and catching site on the gut microbiota of Nile tilapia in Ethiopia. Future work is recommended to precisely explain the causes of these changes using large representative samples of Nile tilapia from different lakes and aquaculture farms.

scholarly journals Dietary Agaricus blazei Spent Substrate Improves Disease Resistance of Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae In Vivo

2022 ◽  
Vol 10 (1) ◽  
pp. 100
Author(s):  
Po-Tsang Lee ◽  
Yu-Sheng Wu ◽  
Chung-Chih Tseng ◽  
Jia-Yu Lu ◽  
Meng-Chou Lee
Keyword(s):  
Cell Line ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Water Extract ◽  
Feed Additive ◽  
Agaricus Blazei ◽  
Bacterial Challenge ◽  
Spent Mushroom Substrate ◽  
Nile Tilapia Oreochromis Niloticus

This study evaluated the effects of the feeding of spent mushroom substrate from Agaricus blazei on Nile tilapia (Oreochromis niloticus). The safety of 0–1000 μg/mL A. blazei spent substrate water extract (ABSSE) was demonstrated in the primary hepatic and splenic macrophages and the THK cell line (a cell line with characteristics of melanomacrophages) using a cytotoxicity assay. Here, 10 μg/mL of crude ABSSE promoted the phagocytic activity of macrophages and THK cells. Stimulating ABSSE-primed THK cells with lipopolysaccharides or peptidoglycan resulted in higher expression levels of four cytokine genes (e.g., interleukinz (IL)-1β, IL-12b, IL-8 and tumor necrosis factor α (TNFα)) and one cytokine gene (TNFα), respectively. An in vitro bacterial growth inhibition assay demonstrated that ABSSE could inhibit the growth of Streptococcus agalactiae. In the first feeding trial, Nile tilapia were fed with experimental feed containing 0, 1, or 5% of A. blazei spent substrate (ABSS) for seven and fourteen days followed by bacterial challenge assay. The best result was obtained when Nile tilapia were continuously fed for seven days on a diet containing 1% ABSS, with the survival rate being higher than in groups with 0% and 5% ABSS after challenge with S. agalactiae. In the second trial, fish were fed diets supplemented with 0% or 1% ABSS for seven days, and then all the groups were given the control feed for several days prior to bacterial challenge in order to investigate the duration of the protective effect provided by ABSS. The results showed that the protective effects were sustained at day 7 after the feed was switched. Overall, spent mushroom substrate from A. blazei is a cost-effective feed additive for Nile tilapia that protects fish from S. agalactiae infection.

scholarly journals Microplastics induced endocrine disruption, alteration in testicular tissue in tilapia (Oreochromis niloticus) pre-fed on Amphora coffeaeformis

2021 ◽  
Author(s):  
Rania F. Ismail ◽  
Norhan E. Saleh ◽  
Alaa El-Din H. Sayed
Keyword(s):  
Endocrine Disruption ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Testicular Tissue ◽  
Degenerative Changes ◽  
Feed Additive ◽  
Negative Effects ◽  
Exposed Fish ◽  
Natural Remedy ◽  
Amphora Coffeaeformis

Abstract This study examines the potential defending effects of the diatom, Amphora coffeaeformis, as a feed additive against the deleterious effects (mainly on gonads) caused by microplastics (MPs) in the Nile tilapia, Oreochromes niloticus. Male tilapia groups were pre-fed diets with four different supplementation levels of A. coffeaeformis (0%, 2.5%, 5% and 7.5%) for 70 days, then were exposed to 10 mg/L MPs for 15 days. The results showed significantly (p < 0.05) higher numbers of erythrocytes, but significant decreases (p < 0.05) in the number of RBCs, Hb, Ht, platelets, and eosinophil percentages. Testicular histological degenerative changes and testis-ova were found in the MPs-exposed fish. Thus, A. coffeaeformis supplementation displayed ameliorative properties that detoxified the negative effects of MPs. This study provides a better understanding of the reproductive injuries caused by MPs and provides evidence for the use of A. coffeaeformis as a natural remedy in freshwater tilapia.

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