Evaluation of in vitro Antifungal Activity of Silver and Selenium Nanoparticles against Alternaria solani Caused Early Blight Disease on Potato

2016 ◽  
Vol 12 (3) ◽  
pp. 1-11 ◽  
Author(s):  
Abdel-Wahab Ismail ◽  
Nagwa Sidkey ◽  
Rawhia Arafa ◽  
Rasha Fathy ◽  
Ahmed El-Batal
Keyword(s):  
Antifungal Activity ◽  
Early Blight ◽  
Alternaria Solani ◽  
Selenium Nanoparticles ◽  
Blight Disease ◽  
In Vitro Antifungal Activity

scholarly journals A Novel Rhizospheric Bacterium, Bacillus Velezensis NKMV-3 as a Biocontrol Agent against Alternaria Leaf Blight in Tomato

2021 ◽  
Author(s):  
Vignesh Murthy ◽  
VedhaHari BodethalaNarayanan ◽  
MubarakAli Davoodbasha ◽  
MadhanShankar ShankarRamakrishanan
Keyword(s):  
Early Blight ◽  
Plant Pathogens ◽  
Alternaria Solani ◽  
Inhibitory Effect ◽  
Rrna Gene ◽  
Bacillus Velezensis ◽  
Bacterium Bacillus ◽  
Blight Disease ◽  
Molecular Sequencing

Abstract A novel strain of Bacillus isolated from rhizosphere has shown to be excellent biocontrol agents against various plant pathogens. In this study, a first report of a Bacillus strain NKMV-3 which effectively controlling Alternaria solani, which cause the Early Blight disease in tomato. Based on the cultural and molecular sequencing of 16S rRNA gene sequence, the identity of the strain was confirmed as Bacillus velezensis NKMV-3. The presence of the lipopeptide which are antibiotic synthesis genes namely Iturin C, Surfactin A, Fengycin B and D were confirmed through gene amplification. In addition, lipopetides was also confirmed through liquid chromatography. The extract showed inhibitory effect against A.solani in-vitro and detached tomato leaf assays. Bacillus velezensis strain NKMV-3 based formulations may provide an effective solution in controlling early blight disease in tomato and other crops.

scholarly journals IN- VITRO ASSAY OF ANTIFUNGAL ACTIVITY OF VARIOUS ELICITORS AND BINDERS AGAINST ALTERNARIA ALTERNATA

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Ruchi Vyas ◽  
Kanika Sharma
Keyword(s):  
Antifungal Activity ◽  
Alternaria Alternata ◽  
Castor Oil ◽  
Effective Control ◽  
Cow Dung ◽  
Vitro Assay ◽  
Blight Disease ◽  
Poison Food Technique ◽  
In Vitro Antifungal Activity

In the present study, in-vitro antifungal activity of various elicitors and binders were evaluated against Alternaria alternata, causative agent of early blight disease in Tomato. Antifungal activities were assayed by poison food technique. Among all elicitors and binders best optimum activity was observed for castor oil cake and cow dung 63.06% and 60 .95% respectively against Alternaria alternata. On the basis of results obtained, best active elicitor i.e., castor oil cake and binder cow dung can be used to develop the plant extract-based bio-formulation for effective control of leaf blight disease of Tomato in an eco-friendly manner.

scholarly journals In vitro antifungal evaluation of various plant extracts against early blight disease (Alternaria solani) of potato

2011 ◽  
Vol 10 (42) ◽  
pp. 8291-8295 ◽  
Author(s):  
Yanar Yusuf ◽  
Gouml kccedil e Ayhan ◽  
Kadioglu Izzet ◽  
Ccedil am Halit ◽  
Whalon Mark
Keyword(s):  
Plant Extracts ◽  
Early Blight ◽  
Alternaria Solani ◽  
Blight Disease

scholarly journals Efficacy of different fungicides for management of early blight disease of potato

2017 ◽  
Vol 9 (1) ◽  
pp. 280-285
Author(s):  
S. Murmu ◽  
S. Dey ◽  
A. Chakraborty
Keyword(s):  
Early Blight ◽  
Alternaria Solani ◽  
Control Measures ◽  
Antifungal Compounds ◽  
Germination Inhibition ◽  
Disease Reduction ◽  
Blight Disease ◽  
Chemical Fungicides

The early blight of potato may be controlled by using bio-control agents and plant extracts but not effectively and rapidly. But using of chemical fungicides the disease can be controlled easily and losses of yield will be reduced compare to above mentioned both control measures. Keeping this in mind an experiment was conducted to test the efficacy of seven different fungicides (difenoconazole, propiconazole, hexaconazole, propineb 61% + iprov-alicarb 5.25%, propineb, carbendazim and metalaxyl 8% + mancozeb 64%) for controlling early blight of potato un-der in vivo as well as in vitro condition. These antifungal compounds were applied 3 times at 7days interval after first appearance of the disease in the field. Highest percent disease reduction (57.88%) and highest yield of tuber (27.03 t/ha) was recorded in plot sprayed with propiconazole @1 ml/lit followed by propineb and propineb 61% + iprovalicarb 5.25%, with percent disease reduction 55.98%, 51.90% and yield 26.30 t/ha and 24.53 t/ha respectively. Simultaneously in vitro efficacy of these fungicides were also tested against Alternaria solani where propiconazole and propineb exhibited highest percent inhibition(100%) in radial growth and in case of spore germination inhibition in spite of these two fungicides difenoconazole, hexaconazole and propineb 61% + iprovalicarb 5.25% exhibit same result i.e. 100% inhibition compared to control as well as other treatments. Therefore it may be suggested that propiconazole and propineb can be used successfully in controlling of this disease.

scholarly journals Synthesis of Bioactive Compounds from 3-Carene (II): Synthesis, Antifungal Activity and 3D-QSAR Study of (Z)- and (E)-3-Caren-5-One Oxime Sulfonates

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 477 ◽  
Author(s):  
Guo-Qiang Kang ◽  
Wen-Gui Duan ◽  
Gui-Shan Lin ◽  
You-Pei Yu ◽  
Xiao-Yu Wang ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Three Dimensional ◽  
Alternaria Solani ◽  
3D Qsar ◽  
Qsar Model ◽  
Quantitative Structure Activity Relationship ◽  
Cercospora Arachidicola ◽  
Qsar Study ◽  
In Vitro Antifungal Activity

A series of novel (Z)- and (E)-3-caren-5-one oxime sulfonates were designed and synthesized in search of potent antifungal agents. The structures of the intermediates and target compounds were confirmed by UV-Vis, FTIR, NMR, and ESI-MS. The in vitro antifungal activity of the target compounds was preliminarily evaluated against Cercospora arachidicola, Physalospora piricola, Alternaria solani, Rhizoeotnia solani, Bipolaris maydis and Colleterichum orbicalare at 50 µg/mL. The bioassay results indicated that the target compounds exhibited the best antifungal activity against P. piricola, in which compounds 4b, 4f, 4m, 4e, 4j, 4l, 4y, 4d, and 4p had excellent inhibition rates of 100%, 100%, 100%, 92.9%, 92.9%, 92.9%, 92.9%, 85.7%, and 85.7%, respectively, showing much better antifungal activity than that of the commercial fungicide chlorothanil. Both the compounds 4y and 4x displayed outstanding antifungal activity of 100% against B. myadis, and the former also displayed outstanding antifungal activity of 100% against R. solani. In order to design more effective antifungal compounds against P. piricola, the analysis of three-dimensional quantitative structure-activity relationship (3D-QSAR) was carried out using the CoMFA method, and a reasonable and effective 3D-QSAR model (r2 = 0.990, q2 = 0.569) has been established.

In vitro Antifungal Activity of a 29-kDa Glycoprotein Purified from the Galls of Quercus infectoria

2005 ◽  
Vol 40 (1-2) ◽  
pp. 43-54 ◽  
Author(s):  
K. Yamunarani ◽  
R. Jaganathan ◽  
R. Bhaskaran ◽  
P. Govindaraju ◽  
R. Velazhahan
Keyword(s):  
Antifungal Activity ◽  
Quercus Infectoria ◽  
In Vitro Antifungal Activity
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