Agrobacterium-Mediated Transfоrmation of Maize with Antisense Suppression of the Proline Dehydrogenase Gene by an In Planta Method

2014 ◽  
Vol 4 (2) ◽  
pp. 116-125 ◽  
Author(s):  
Y. Moiseeva
Keyword(s):  
Proline Dehydrogenase ◽  
In Planta ◽  
Antisense Suppression ◽  
Dehydrogenase Gene

Cloning and Expression of a Proline Dehydrogenase Gene fromCelastrus orbiculatus

2014 ◽  
Vol 49 (6) ◽  
pp. 643
Author(s):  
Wang Cuili ◽  
Tian Haixia ◽  
Wang Jiaojiao ◽  
Qi Tiancong ◽  
Huang Huang ◽  
...  
Keyword(s):  
Cloning And Expression ◽  
Proline Dehydrogenase ◽  
Dehydrogenase Gene

A family-based association study of T1945C polymorphism in the proline dehydrogenase gene and schizophrenia in the Chinese population

2003 ◽  
Vol 338 (3) ◽  
pp. 252-254 ◽  
Author(s):  
Jin-Bo Fan ◽  
Jie Ma ◽  
Chang-Shun Zhang ◽  
Jun-Xia Tang ◽  
Niu-Fan Gu ◽  
...  
Keyword(s):  
Association Study ◽  
Chinese Population ◽  
Proline Dehydrogenase ◽  
Dehydrogenase Gene ◽  
Family Based

Evaluation of salt tolerance in Nicotiana tabacum plants bearing an antisense suppressor of the proline dehydrogenase gene

2006 ◽  
Vol 42 (2) ◽  
pp. 212-214 ◽  
Author(s):  
Ya. S. Kolodyazhnaya ◽  
S. E. Titov ◽  
A. V. Kochetov ◽  
M. L. Komarova ◽  
A. V. Romanova ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Salt Tolerance ◽  
Proline Dehydrogenase ◽  
Dehydrogenase Gene

scholarly journals Relationship between polymorphisms in the proline dehydrogenase gene and schizophrenia risk

2015 ◽  
Vol 14 (4) ◽  
pp. 11681-11691 ◽  
Author(s):  
F. Ghasemvand ◽  
E. Omidinia ◽  
Z. Salehi ◽  
S. Rahmanzadeh
Keyword(s):  
Proline Dehydrogenase ◽  
Dehydrogenase Gene

scholarly journals Corn plant comparative reactions to artificial dehydration

1970 ◽  
pp. 252-255
Author(s):  
L. Ye. Serhieieva ◽  
S. I. Mykhalska ◽  
V. M. Kurchii ◽  
O. M. Tyshchenko
Keyword(s):  
Water Stress ◽  
Free Proline ◽  
In Planta ◽  
Short Term ◽  
Agrobacterium Mediated Transformation ◽  
Water Stress Tolerance ◽  
Dehydrogenase Gene ◽  
Maize Plants ◽  
The Stability ◽  
High Level

Aim. There are maize plants of inbred line L-370 (control) and from T4 progeny of plants transformed via in planta Agrobacterium-mediated transformation with LBA4404 strain harboring pBi2E with double-stranded RNA-suppressor of the proline dehydrogenase gene. The free proline and sucrose levels, sucrose/fructose ratio during period of desicca-tion/rehydration were investigated. Methods. Maize T4 progeny and initial plants of L-370 line were cultivated in con-tainers. Variants were tested under short term (4 days) dehydration and after 3.5 hours of rehydration. The free proline and carbohydrates levels were estimated at those times of the experiment. Results. The short-term water deficit did not create pathological changes in plants. But the free proline levels rose in leaves of all variants. At the same time the ami-no acid levels under stress condition in T4 plans were higher than in L-370-plants. After 3.5 hours of rehydration the proline content in control plants decreased and stayed without changes in T4 plans. In T4 plants the carbohydrate meta-bolism systems energy maintained the stability of the sucrose/fructose ratio during the whole time of experiment. Conclusions. The T4 plants high level of water stress tolerance is a possible result of transgene activity.Keywords: Zea mays, Agrobacterium-mediated transformation, T4-progeny, short-term water stress, dehydration, proline sucrose.

scholarly journals Genetic and physiological analysis T1 biotechnological plants of winter wheat (Triticum aestivum L.)

2020 ◽  
Vol 26 ◽  
pp. 222-227
Author(s):  
A. G. Komisarenko ◽  
S. I. Mykhalska ◽  
V. M. Kurchii ◽  
O. O. Khrystan
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
First Generation ◽  
Genetically Modified ◽  
Molecular Genetic ◽  
Triticum Aestivum L ◽  
Molecular Genetic Analysis ◽  
Pcr Analysis ◽  
Proline Dehydrogenase ◽  
In Planta

Aim. To investigate inheritance of transgenes in the first generation (T1) of winter wheat biotechnological plants (Triticum aestivum L.). Analyze the performance of T1 genetically modified plants with a double stranded RNA suppressor of the proline dehydrogenase (pdh) gene under normal growing conditions. Methods. PCR analysis, DNA electrophoresis; determination of indicators of the structure of the crop. Results. Molecular genetic analysis was performed and the performance indicators of control and T1 biotechnological plants were investigated. Conclusions. The first generation of genetically modified winter wheat plants resulting from Agrobacterium-mediated transformation in planta confirmed the inheritance of integrated genes. Among the transgenic variants identified plants that lack some fragments of the target gene required for partial suppression of the gene of proline dehydrogenase wheat. It is shown that at the optimal terms of growing biotechnological plants of wheat winter-annual of UK 322/17 and UK 209 h was characterized by the best indexes of structure of harvest as compared to an initial form, while the genetically changed plants of genotypes of UK 95/17 and UK 065 after the elements of the productivity did not differ from control plants. Keywords: Triticum aestivum L., biotechnological plants, T-DNA, proline dehydrogenase gene, structural analysis indicators.

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