scholarly journals Influence of Type and Age of Primary Somatic Embryo on Secondary and Cyclic Somatic Embryogenesis of Cassava (Manihot esculenta Crantz)

2014 ◽  
Vol 4 (3) ◽  
pp. 254-269 ◽  
Author(s):  
Jelili Opabode
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Manihot Esculenta ◽  
Manihot Esculenta Crantz ◽  
Primary Somatic Embryo

scholarly journals Induction of cassava somatic embryogenesis in liquid medium associated to floating membrane rafts

2000 ◽  
Vol 43 (1) ◽  
Author(s):  
Elizabete Keiko Takahashi ◽  
Adilson Kenji Kobayashi ◽  
Luiz Gonzaga Esteves Vieira
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Manihot Esculenta ◽  
Solid Medium ◽  
Young Leaf ◽  
Membrane Rafts ◽  
Manihot Esculenta Crantz ◽  
Lower Response ◽  
Culture Systems

The objective of this study was to examine the effect of two culture systems, liquid medium associated to floating membranes and solid medium, both supplemented with different concentrations of 2,4-D, in the induction of somatic embryogenesis of cassava (Manihot esculenta Crantz). Only 28% of the young leaf lobes (with 9 µM 2,4-D) were induced to form organized embryogenic structures (OES) with membrane rafts, compared to 50% of the explants presenting this type of tissue in solid medium with 36 µM of 2,4-D. Despite the lower response observed in liquid medium with membrane, the amount of OES/explant in all 2,4-D concentrations was higher than solid medium. Based on the results and considering the high cost of the membrane rafts, this system was not distinctly superior than solid medium for inducing somatic embryogenesis in cassava.

scholarly journals SOMATIC EMBRYOGENESIS OF CASSAVA (MANIHOT ESCULENTA CRANTZ) VAR. KM297

2011 ◽  
Vol 14 (3) ◽  
pp. 14-22
Author(s):  
Kien Van Vu ◽  
Sanh Du Nguyen
Keyword(s):  
Somatic Embryogenesis ◽  
Manihot Esculenta ◽  
Somatic Embryos ◽  
Light Condition ◽  
Ms Medium ◽  
Dark Condition ◽  
Manihot Esculenta Crantz ◽  
Immature Leaf

Somatic embryos of cassava var. KM297 received from pieces of in vitro immature leaf lobes or cotyledon of somatic embryos, were induced on the MS medium supplemented with 8mg/l picloram after 13 days inoculation in the dark condition. Different states of embryo were obtained after 10 days cultured on MS medium supplemented with 0.1 mg/l BA and 0.01mg/l NAA, in the light condition. Role of endogenous AIA and Zeatin of the globular state of embryos was studied.

scholarly journals Optimization of Somatic Embryogenesis Induction of Cassava (Manihot esculenta Crantz)

2018 ◽  
Vol 21 (2) ◽  
pp. 45
Author(s):  
Idha Susanti ◽  
Suharsono Suharsono ◽  
Utut Widyastuti ◽  
Ulfah Juniarti Siregar ◽  
Aris Tjahjoleksono
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Manihot Esculenta ◽  
Callus Formation ◽  
Induction Treatment ◽  
Manihot Esculenta Crantz ◽  
Friable Callus ◽  
Apical Bud ◽  
Different Response

The embryogenesis (SE) has important role for genetic engineering of cassava (Manihot esculenta Crantz). However, the success of SE induction depend on plant growth regulator s (PGR)s and treatment enriched in induction media.  This experiment tried to induce callus formation of cassava from several in vitro explants: immature leaf, apical bud, and internode; and to develop somatic embryogenesis of cassava in several media enriched with tyrosine and copper sulphate (CuSO4) added into media enrich with picloram as treatment.  Different response of explants source to callus induction treatment from those three varieties in callus induction as well as friable callus formation were found in this experiment. The best medium to induce varied with variety; MS media supplemented 12 mg/L picloram + 0.5 mg/L CuSO4 was the best for “Adira 4” and  half MS and half GD media supplemented 12 mg/L picloram + 100 mg/L tyrosine for “Malang 6”.  All treatments resulted somatic embryo which developed indirectly and in morphologically normal somatic embryos

Improvements of cyclic somatic embryogenesis of cassava (Manihot esculenta Crantz)

1993 ◽  
Vol 12 (4) ◽  
Author(s):  
C.J.J.M. Raemakers ◽  
C.M. Schavemaker ◽  
E. Jacobsen ◽  
R.G.E. Visser
Keyword(s):  
Somatic Embryogenesis ◽  
Manihot Esculenta ◽  
Manihot Esculenta Crantz

scholarly journals Somatic Embryogenesis in Hedychium bousigonianum

HortScience ◽  
2009 ◽  
Vol 44 (5) ◽  
pp. 1487-1490 ◽  
Author(s):  
Hamidou F. Sakhanokho ◽  
Kanniah Rajasekaran ◽  
Rowena Y. Kelley
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryo ◽  
Culture Media ◽  
Callus Growth ◽  
Dichlorophenoxyacetic Acid ◽  
Friable Callus ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Basal Salts ◽  
Primary Somatic Embryo ◽  
Better Than

An efficient primary somatic embryo (SE) and secondary somatic embryo (SSE) production system was developed for the ornamental ginger Hedychium bousigonianum Pierre ex Gagnepain. Addition of two ethylene inhibitors, salicylic acid (SA) and silver nitrate (AgNO3), to the culture media improved the system. Callus was initiated and proliferated on a medium containing Murashige and Skoog (MS) basal salts supplemented with 9.05 μM 2,4-dichlorophenoxyacetic acid and 4.6 μM kinetin. Friable callus was transferred to a liquid medium containing MS basal salts, B5 vitamins, 0.6 μM thidiazuron, and 8.9 μM 6-benzylaminopurine to induce somatic embryogenesis. The effects of various concentrations of SA (0, 25, 50, 75, 100, 125, 150 μM) and AgNO3 (0, 10, 20, 30, 40, 50, 60 μM) on callus growth, SE, and SSE development was further evaluated. The rate of callus growth decreased as the concentrations of SA or AgNO3 increased. AgNO3 and SA at all concentrations stimulated SE and SSE development better than the control although a decrease in embryo production was observed at higher concentrations of both SA and AgNO3. The best concentrations for SA were 75 and 100 μM, whereas for AgNO3, they were 30 to 50 μM for both SE and SSE production.

Sign in / Sign up

Export Citation Format

Share Document