scholarly journals Chemical Properties and DPPH Radical Scavenging Ability of Sword Bean (Canavalia gladiata) Extract

2012 ◽  
Vol 44 (4) ◽  
pp. 441-446 ◽  
Author(s):  
Jong-Pil Kim ◽  
Yong-Shik Yang ◽  
Jin-Hee Kim ◽  
Hyang-Hee Lee ◽  
Eun-Sun Kim ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Chemical Properties ◽  
Dpph Radical ◽  
Canavalia Gladiata ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

scholarly journals THE CHARACTERISTICS AND ANTIOXIDANT ACTIVITIES OF CHABA MAPLE (HIBISCUS ACETOSELLA) HOMEMADE JAM

2019 ◽  
pp. 52-55
Author(s):  
SURADWADEE THUNGMUNGMEE ◽  
NAKUNTWALAI WISIDSRI ◽  
WARACHATE KHOBJAI ◽  
THISAKORN DUMRONGPHUTTIDECHA ◽  
KHEMJIRA JAMKOM ◽  
...  
Keyword(s):  
High Performance ◽  
Antioxidant Activities ◽  
Sugar Content ◽  
Radical Scavenging ◽  
Chemical Properties ◽  
Chemical Characteristics ◽  
Dpph Radical ◽  
Antioxidant Power ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Objective: This study aimed to characterize physicochemical and chemical characteristics of Chaba maple (Hibiscus acetosella) homemade jam (CHJ)and determine its autoxidation ability.Methods: The physicochemical and chemical characteristics of CHJ were investigated. The color, viscosity, and pH were observed as physicochemicaldata while chemical properties were obtained from sugar content and total polyphenol content (TPC), determined using high-performance liquidchromatography refractometer and Folin–Ciocalteu assay, respectively. The antioxidant activities of CHJ were identified using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and nitric oxide (NO) radical scavenging ability methods.Results: The color and viscosity of CHJ were purple-red and 34,483.33±152.75 cP, respectively. The pH was at 3.78. The total sugar was not detectedin CHJ. The TPC of CHJ showed the highest (47.18±1.80 mg gallic acid equivalent [GAE]/g of jam) followed by Streamline (SL) (23.66±0.32 mgGAE/g of jam), Doikham (DK) (21.99±0.50 mg GAE/g of jam), and Best food (BF) (9.75±0.38 mg GAE/g of jam), respectively. Antioxidant activitiesof CHJ with %1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging of 100.00±1.39% and FRAP value of 1690.70±8.26 uM. Both of activitiesexhibited the highest activity and significantly different when compared with other jams. The %NO scavenging activity of CHJ and SL was 72.43±1.93%and 73.82±1.66%, respectively, which higher than DK and BF.Conclusion: This study shows good in both physicochemical and chemical characteristics of CHJ. The CHJ presents the highest TPC as well asantioxidant activities. Thus, a homemade jam of Chaba maple may be considered as a good source of antioxidants and functional foods.

scholarly journals Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 592 ◽  
Author(s):  
Chengli Hou ◽  
Liguo Wu ◽  
Zhenyu Wang ◽  
Elena Saguer ◽  
Dequan Zhang
Keyword(s):  
Liquid Chromatography ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Amino Acid Sequences ◽  
Dpph Radical ◽  
Antioxidant Peptides ◽  
Animal Blood ◽  
Antioxidant Power ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

In this study, sheep plasma was submitted to Alcalase-hydrolysis and peptides with better antioxidant properties measured through both the ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability assays were isolated and identified. After hydrolysate ultrafiltration and semi-preparative reverse-phase high-performance liquid chromatography, nine fractions (F1–F9) were obtained, with the two first (F1 and F2) showing the greatest antioxidant potential. These two fractions were further separated by the AKTA purifier system to generate four (F1-1–F1-4) and five (F2-1–F2-5) fractions, respectively, with two of them (F1-2 and F2-1) exhibiting appreciable FRAP activity and DPPH radical scavenging ability. Using liquid chromatography-tandem mass spectrometry, three antioxidant peptides were identified. From their amino acid sequences (QTALVELLK, SLHTLFGDELCK, and MPCTEDYLSLILNR), which include amino acids that have been previously reported as key contributors to the peptide antioxidant properties, it can be maintained that they come mainly from serum albumin. These results suggested that the sheep plasma protein can be considered as a good source of antioxidant peptides and bring forth new possibilities for the utilization of animal blood by-products.

Antioxidant Activities of Crude Extract and Chromatographic Fraction from Hsian-Tsao (Mesona Procumbens Hemsl.)

2012 ◽  
Vol 550-553 ◽  
pp. 1784-1789 ◽  
Author(s):  
Ting Qiu ◽  
Bi Yu Wang ◽  
Xiao Cui Lin ◽  
Yan Xiang Wu
Keyword(s):  
Lipid Peroxidation ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Dpph Radical ◽  
Chelating Ability ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

This study aimed at evaluating the antioxidative activity of crude hsian-tsao extracted by acetone and the Fraction E purified by AB-8 macroporous resin chromatography column. The antioxidative activities, including the DPPH radical-scavenging effects, Fe2+-chelating ability, and reducing power as well as the inhibition of lipid peroxidation, were studied. Results demonstrated that both crude extract and Fraction E were more effective in the inhibition of lipid peroxidation and the DPPH radical-scavenging ability than that in the Fe2+-chelating ability. Based on the inhibition of lipid peroxidation, the order of the antioxidant activities was follows: Fraction E >GA> crude extract >Vc. Fraction E seemed to be a promising product of natural antioxidants enriched with phenolic compounds

Preparation and Properties of Complex Antioxidants LDPE Antioxidant Film

2014 ◽  
Vol 989-994 ◽  
pp. 519-522
Author(s):  
Hui Yang ◽  
Li Li ◽  
Fu Xin Yang ◽  
Ying Yue Zhou ◽  
Li Juan Ou ◽  
...  
Keyword(s):  
Transmission Rate ◽  
Radical Scavenging ◽  
Low Density Polyethylene ◽  
Low Density ◽  
Water Vapor Transmission Rate ◽  
Dpph Radical ◽  
Water Vapor Transmission ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging ◽  
Scanning Electron

Low density polyethylene (LDPE) films contains BHA, BHT or BHA, BHT, TA were successfully produced by extrusion. Three types of antioxidant films that were L(LDPE), LBB(LDPE+0.5%BHA+0.5%BHT), and LBBT(LDPE+0.5%BHA+0.5%BHT+0.5%TA) was designed. The Mechanics, moisture permeability, scanning electron microscopy (SEM) and antioxidant activity of the film were detected. The results showed that antioxidant consisting of BHA, BHT and TA has a better compatibility with LDPE film. The antioxidant film has a better performance on mechanical strength and the rate of DPPH radical scavenging ability, but poor to water vapor transmission rate.

Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O’Rorke) Baill bark

2018 ◽  
Vol 29 (2) ◽  
pp. 217-224 ◽  
Author(s):  
Oluwafemi Adeleke Ojo ◽  
Adebola Busola Ojo ◽  
Basiru Olaitan Ajiboye ◽  
Babatunji Emmanuel Oyinloye ◽  
Ayodele Jacob Akinyemi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Dependent Manner ◽  
Dpph Radical ◽  
Irvingia Gabonensis ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging ◽  
Phenolic Extracts

AbstractBackground:Irvingia gabonensisstem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis. This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts fromI. gabonensis(Aubry-Lecomte ex O‘Rorke) Baill bark.Methods:Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability. Inhibitory activities on AChE and BChE were evaluated.Results:The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin). The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+to Fe2+) (5.94 mg ascorbic acid equivalent/100 g). Extracts ofI. gabonensisinhibited AChE (IC50=32.90 μg/mL) and BChE (IC50=41.50 μg/mL) activities in concentration-dependent manner.Conclusions:Hence, possible mechanism through which the stem bark executes their anti-Alzheimer’s disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.

scholarly journals Antimicrobial, Antioxidative, Elastase and Tyrosinase Inhibitory Effect of Supercritical and Hydrothermal Halopteris scoparia Extract

2021 ◽  
Vol 12 (5) ◽  
pp. 407-413
Author(s):  
Soo Hyeon Heo, Su In Park, Jinseo Lee, Sung Won Jung,Moon Sam Shin
Keyword(s):  
Propionibacterium Acnes ◽  
Radical Scavenging ◽  
Inhibitory Effect ◽  
Dpph Radical ◽  
Tyrosinase Inhibition ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Halopteris Scoparia ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

To find out a good candidate of cosmetic source and screened for antimicrobial, antioxidant and whitening activities. In the antioxidant experiment, supercritical Halopteris scoparia extraction (SHE) and hydrothermal Halopteris scoparia extraction (HHE) were slightly different depending on the experimental method (Total polyphenol content measurement, DPPH radical scavenging ability, ABTS radical scavenging ability, and SOD-like activity) and in the enzyme experiment (Elastase, Tyrosinase inhibition activity), the supercritical extract was more effective. In supercritical fluid, Halopteris scoparia extracts showed good antimicrobial activity against Propionibacterium acnes, Staphylococcus aureus, Bacillus subtilis and Escherichia coli but hydrothermal extract had no effect about the rest of the strains. Therefore, it can be expected that the supercritical Halopteris scoparia extract with excellent antibacterial activity can be used as a cosmetic material.

Production and Preliminary Characterization of Antioxidant Polysaccharide by Submerged Culture of Culinary and Medicinal Fungi Cordyceps militaris CICC14013

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Wan Chen ◽  
Gaoqiang Liu ◽  
Huandong Yang ◽  
Zhichao Wu ◽  
Hailong Yang
Keyword(s):  
Submerged Culture ◽  
Biological Activities ◽  
Average Molecular Weight ◽  
Radical Scavenging ◽  
Deae Cellulose ◽  
Reducing Power ◽  
Cordyceps Militaris ◽  
Dpph Radical ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Abstract Polysaccharides from Cordyceps militaris show multiple biological activities, such as antioxidation, antitumor, antivirus and immunomodulating properties, etc. The nutritional requirements for antioxidant polysaccharide bio-synthesis by submerged culture of C. militaris were evaluated on the basis of assay for DPPH radical scavenging ability and reducing power in this work. The results showed the best antioxidant activity of polysaccharide could be achieved when the media consisted of mannose, peptone, MgSO4, Na2HPO4, and KH2PO4. Furthermore, the main fraction of antioxidant polysaccharide, CMP-2, was preliminary isolated from the fermentation broth of C. militaris by ethanol precipitation, filtration, and DEAE cellulose-52 chromatography. CMP-2 was composed of mannose, rhamnose, glucose and galactose in a ratio of 2527:164:172:100, and had an average molecular weight (Mw) of approximately 8175 Da. The results suggested antioxidant polysaccharide could be produced by submerged culture of C. militaris and the medium composition significantly affected its DPPH radical scavenging ability and reducing power.

scholarly journals Conventional and Enzyme-Assisted Extraction of Rosemary Leaves (Rosmarinus officinalis L.): Toward a Greener Approach to High Added-Value Extracts

2021 ◽  
Vol 11 (8) ◽  
pp. 3724
Author(s):  
Antonella Rozaria Nefeli Pontillo ◽  
Lydia Papakosta-Tsigkri ◽  
Theopisti Lymperopoulou ◽  
Diomi Mamma ◽  
Dimitris Kekos ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Added Value ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Assisted Extraction ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging ◽  
Enzyme Assisted Extraction

The effect of different extraction methods of rosemary leaves on the total phenolic content (TPC), and the antioxidant activity of the extracts was herein investigated. Firstly, the solid-liquid conventional extraction (CEM) and microwave-assisted extraction (MAE) were implemented in an effort to identify the effect of the solvent and of microwave irradiation on the extract quality. The extract obtained from CEM at room temperature, using ethanol/water 95:5 v/v, showed the highest antioxidant activity (IC50 = 12.1 μg/mL). MAE using ethanol/water 50:50 v/v provided an extract with TPC and DPPH radical scavenging ability in a significantly shorter extraction time (1 h for MAE and 24 h for CEM). Enzyme-assisted extraction (EAE) using five commercial enzyme formulations was implemented, and the kinetic equation was calculated. Finally, the effect of EAE as a pretreatment method to CEM was examined. Pretreatment of the plant material with pectinolytic enzymes for 1 h prior to a 24 h CEM with 50% hydroethanolic solvent was found to be the optimum conditions for the extraction of rosemary leaves, providing an extract with higher DPPH radical scavenging ability (IC50 14.3 ± 0.8 μg/mL) and TPC (15.2 ± 0.3 mgGAE/grosemary) than the corresponding extract without the enzyme pretreatment.

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