Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O’Rorke) Baill bark

2018 ◽  
Vol 29 (2) ◽  
pp. 217-224 ◽  
Author(s):  
Oluwafemi Adeleke Ojo ◽  
Adebola Busola Ojo ◽  
Basiru Olaitan Ajiboye ◽  
Babatunji Emmanuel Oyinloye ◽  
Ayodele Jacob Akinyemi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Dependent Manner ◽  
Dpph Radical ◽  
Irvingia Gabonensis ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging ◽  
Phenolic Extracts

AbstractBackground:Irvingia gabonensisstem bark is a medicinal plant used in most parts of Africa to manage a number of ailments including neurodegenerative diseases that occur without scientific basis. This work characterized the phenolic composition, evaluated the cholinergic enzymes (acetylcholinesterase, AChE and butyrylcholinesterase, BChE) inhibition, and assessed the antioxidant activity of phenolic extracts fromI. gabonensis(Aubry-Lecomte ex O‘Rorke) Baill bark.Methods:Total phenol and flavonoids content was evaluated in addition to antioxidant activity as shown by Fe2+chelation, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, and 2,2-azino-bis-(3-ethylbenthiazoline-6-sulfonic acid) (ABTS) radical scavenging ability. Inhibitory activities on AChE and BChE were evaluated.Results:The extract was found to be rich in phenolic acid (ellagic acid) and flavonoids (quercetrin, kaempferol, and apigenin). The phenolic extracts displayed DPPH radical scavenging ability (IC50=19.98 μg/mL), ABTS radical scavenging ability (IC50=18.25 μg/mL), iron chelation (IC50=113.10 μg/mL), and reducing power (Fe3+to Fe2+) (5.94 mg ascorbic acid equivalent/100 g). Extracts ofI. gabonensisinhibited AChE (IC50=32.90 μg/mL) and BChE (IC50=41.50 μg/mL) activities in concentration-dependent manner.Conclusions:Hence, possible mechanism through which the stem bark executes their anti-Alzheimer’s disease activity might be by inhibiting cholinesterase activities in addition to suppressing oxidative-stress-induced neurodegeneration.

scholarly journals Phytochemical screening and antioxidant activity of Melastoma malabathricum and Chromolaena odorata by DPPH radical scavenging method

Food Research ◽  
2021 ◽  
Vol 5 (S4) ◽  
pp. 30-37
Author(s):  
N.H. Ismail ◽  
Amira N.H. ◽  
S.N.H.M. Latip ◽  
W.Z.W.M. Zain ◽  
S.N.A. Aani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Nutritive Value ◽  
Chemical Constituents ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dpph Radical ◽  
Chromolaena Odorata ◽  
Melastoma Malabathricum ◽  
Dpph Radical Scavenging

Melastoma malabathricum and Chromolaena odorata are classified under broad-leaved weeds that are widely spread in the open land area. Melastoma malabathricum is commonly known as ‘‘senduduk’’, and C. odorata is locally known as “Pokok Kapal Terbang”. Both weeds are categorized as potential weeds as they have high nutritive value and are rich in chemical compounds. This study aimed to determine their chemical constituents and possible potential for antioxidant activity as these weeds have been reported to possess antioxidant properties. Screening of the plants was performed using standard methods and revealed the existence of various secondary metabolites such as saponins, terpenoids, phenols, tannins, and flavonoids of both weed extracts. Antioxidant activity was validated by the DPPH radical scavenging assay of M. malabathricum and C. odorata crude ethanol extract. The IC50 values for the percentage radical scavenging effects for the extracts were determined. The IC50 value of M. malabatrichum extract was 81.116 μg/mL, C. odorata was 312.903 μg/mL, Vitamin C was 31.023 μg/mL and BHA was 71.521 μg/mL respectively. The study showed that the antioxidant activity of M. malabatrichum was more potent and better than C. odorata.

scholarly journals Purification and Identification of Antioxidant Alcalase-Derived Peptides from Sheep Plasma Proteins

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 592 ◽  
Author(s):  
Chengli Hou ◽  
Liguo Wu ◽  
Zhenyu Wang ◽  
Elena Saguer ◽  
Dequan Zhang
Keyword(s):  
Liquid Chromatography ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Amino Acid Sequences ◽  
Dpph Radical ◽  
Antioxidant Peptides ◽  
Animal Blood ◽  
Antioxidant Power ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

In this study, sheep plasma was submitted to Alcalase-hydrolysis and peptides with better antioxidant properties measured through both the ferric-reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability assays were isolated and identified. After hydrolysate ultrafiltration and semi-preparative reverse-phase high-performance liquid chromatography, nine fractions (F1–F9) were obtained, with the two first (F1 and F2) showing the greatest antioxidant potential. These two fractions were further separated by the AKTA purifier system to generate four (F1-1–F1-4) and five (F2-1–F2-5) fractions, respectively, with two of them (F1-2 and F2-1) exhibiting appreciable FRAP activity and DPPH radical scavenging ability. Using liquid chromatography-tandem mass spectrometry, three antioxidant peptides were identified. From their amino acid sequences (QTALVELLK, SLHTLFGDELCK, and MPCTEDYLSLILNR), which include amino acids that have been previously reported as key contributors to the peptide antioxidant properties, it can be maintained that they come mainly from serum albumin. These results suggested that the sheep plasma protein can be considered as a good source of antioxidant peptides and bring forth new possibilities for the utilization of animal blood by-products.

Synthesis, Photophysical, and Antioxidant Properties of Rhodamine B Decorated Novel Dendrimers

2018 ◽  
Vol 71 (6) ◽  
pp. 399 ◽  
Author(s):  
Jothinathan Sathiya Savithri ◽  
Perumal Rajakumar
Keyword(s):  
Antioxidant Activity ◽  
Fluorescence Intensity ◽  
Rhodamine B ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Surface Groups ◽  
Dpph Radical ◽  
Assay Methods ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

Novel triazole bridged dendrimers with rhodamine B derivative as surface groups have been achieved using click chemistry by both divergent and convergent approaches. Rhodamine B decorated dendrimers 1, 2, and 3 were synthesised up to the second generation with spirolactam grafted at the terminal. The UV and fluorescence intensity increases with the increase in the dendritic generation. The synthesised rhodamine B decorated dendrimers show significant antioxidant behaviour compared with the standards butylated hydroxy toluene (BHT) and gallic acid when tested by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging assay and hydroxyl radical scavenging assay methods, respectively. Rhodamine B decorated higher generation dendrimers exhibit better antioxidant activity than the lower generation dendrimers due to the presence of a greater number of triazole branching units and rhodamine B derivative surface units.

scholarly journals Chemical Investigation of SomeCapparisSpecies Growing in Egypt and their Antioxidant Activity

2007 ◽  
Vol 4 (s1) ◽  
pp. 25-28 ◽  
Author(s):  
Ahmed R. Hamed ◽  
Khaled A. Abdel-Shafeek ◽  
Nahla S. Abdel-Azim ◽  
Shams I. Ismail ◽  
Faiza M. Hammouda
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Evaluation ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Chemical Investigation ◽  
Dpph Radical ◽  
Densitometric Analysis ◽  
Butanol Fraction ◽  
First Time ◽  
Dpph Radical Scavenging

Capparis cartilagineaandC. desertigrowing in Egypt were investigated for their glucosiolates and rutin content. FromCapparis cartilagineafour isothiocynates were isolated and identified using GC and EI/MS techniques. These compounds were butyl isothiocyanate (1), 6-methylsulphonylhexyl isothiocyanate (2), 7-methylsulphonylheptyl isothiocyanate (3) and 5-benzylsulphonyl-4-pentenyl isothiocyanate (4). In addition to compounds (1) and (2), two other compounds were isolated and identified fromCapparis deserti. These compounds are 3-methylthiopropyl isothiocyanate (5) and [11-(2-butenylthio)6-undecenyl isothiocyanate] (6). Compounds (1), (2), (5) and (6) are reported in this study for the first time fromCapparis deserti. The main flavonoid component in the studied species was isolated and identified as rutin by comparing the data with those reported. Also, quantitative evaluation of rutin in the two species was carried out by TLC-densitometric analysis. The antioxidant activity was done using diphenylpicrylhydrazyl (DPPH) radical scavenging method. The butanol fraction fromC. cartilagineaandC. desertishowed the highest antioxidant properties.

scholarly journals (464) Antioxidant Activity in Lingonberries (Vaccinium vitis-idaea L.)

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1090C-1090 ◽  
Author(s):  
Shiow Wang ◽  
Ross Penhallegon
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Soluble Solids ◽  
Oh Radicals ◽  
Dpph Radical ◽  
Phenolic Contents ◽  
Solids Content ◽  
Dpph Radical Scavenging

Lingonberries have been shown to contain high antioxidant activity. Fruit from different cultivars of lingonberry (Vacciniumvitis-idaea L) were evaluated for fruit quality, antioxidant activity, anthocyanin and phenolic contents. The fruit soluble solids content (SSC), titratable acids (TA), antioxidant capacity, anthocyanin and phenolic contents varied with cultivars. The SSC ranged from 12.9% to 16.9%, the TA ranged from 0.31% to 0.41% and the ratios of SSC/TA ranged from 35.37 to 51.21. Lingonberries contain potent free radical scavenging activities for DPPH·, ROO·, ·OH and O .-2 radicals. The oxygen radical absorbance capacity (ORAC) values from acetone extraction of lingonberries ranged from 58.5–223.6 μmol of Trolox equivalents (TE)/g fresh berries. The ED50 values for DPPH-radical scavenging ranged from 5.91–11.77 mg fresh weight. The DPPH-radical scavenging activity correlated with ORAC values with a R2 of 0.8009. ESR spectrum showed that 50 mg·mL-1 of lingonberry extract decreased ·OH radicals by 83% and O .-2 radicals by 99%. Cyanidin 3-galactoside was the most dominant anthocyanin and contributed the most antioxidant activity in lingonberries. The antioxidant properties of lingonberries may play an important role in protecting cells against the oxidative damage caused by free radicals.

scholarly journals A preliminary investigation of anticholinesterase and antioxidant properties of various extracts of Vernonia anthelmintica in relation to the treatment of Alzheimer’s disease

2020 ◽  
Vol 11 (3) ◽  
pp. 4511-4517
Author(s):  
Swati Som ◽  
Justin Antony ◽  
Palanisamy Dhanabal S ◽  
Sivasankaran Ponnusankar
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Ache Inhibition ◽  
Dpph Radical ◽  
Vernonia Anthelmintica ◽  
Dpph Radical Scavenging

The complexity of the underlying pathogenesis of Alzheimer’s disease (AD) and substandard results of existing treatment, demands persistent research for the development of new therapeutics. As natural antioxidant has attracted considerable attention on this regards, the present study evaluated and validated antioxidant and anti-cholinesterase activity of Vernonia anthelmintica seeds. Different extracts were collected after sequential extraction of plant seeds by using pet ether, chloroform, ethyl acetate, methanol and water as solvents. Antioxidant activity of the extracts was tested by using superoxide, nitric oxide (NO) and 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays, whereas, acetylcholine esterase (AChE) inhibition property was assessed by Ellman’s method. Results revealed that all the extracts produced some degree of both the activities in a concentration-dependent manner. While pet ether extract exhibited weakest, methanolic extract displayed potent radical scavenging and anti-cholinesterase properties in all the assays with IC50 of 159.71 μg/ml against AChE inhibition and 98.51, 120.22 and 170.79 μg/ml against superoxide, NO and DPPH radical scavenging assays respectively. Presence of an array of secondary metabolites with modest flavonoid and phenol content in the methanolic extract is accountable for these desired activities. Collectively, reports from our experiments covey that V. anthelmintica possess significant AChE inhibitory and antioxidant property and thus can be further evaluated in search of potential disease-modifying therapeutic for management of neurodegenerative diseases like AD.

scholarly journals Conventional and Enzyme-Assisted Extraction of Rosemary Leaves (Rosmarinus officinalis L.): Toward a Greener Approach to High Added-Value Extracts

2021 ◽  
Vol 11 (8) ◽  
pp. 3724
Author(s):  
Antonella Rozaria Nefeli Pontillo ◽  
Lydia Papakosta-Tsigkri ◽  
Theopisti Lymperopoulou ◽  
Diomi Mamma ◽  
Dimitris Kekos ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Extraction Methods ◽  
Added Value ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Assisted Extraction ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging ◽  
Enzyme Assisted Extraction

The effect of different extraction methods of rosemary leaves on the total phenolic content (TPC), and the antioxidant activity of the extracts was herein investigated. Firstly, the solid-liquid conventional extraction (CEM) and microwave-assisted extraction (MAE) were implemented in an effort to identify the effect of the solvent and of microwave irradiation on the extract quality. The extract obtained from CEM at room temperature, using ethanol/water 95:5 v/v, showed the highest antioxidant activity (IC50 = 12.1 μg/mL). MAE using ethanol/water 50:50 v/v provided an extract with TPC and DPPH radical scavenging ability in a significantly shorter extraction time (1 h for MAE and 24 h for CEM). Enzyme-assisted extraction (EAE) using five commercial enzyme formulations was implemented, and the kinetic equation was calculated. Finally, the effect of EAE as a pretreatment method to CEM was examined. Pretreatment of the plant material with pectinolytic enzymes for 1 h prior to a 24 h CEM with 50% hydroethanolic solvent was found to be the optimum conditions for the extraction of rosemary leaves, providing an extract with higher DPPH radical scavenging ability (IC50 14.3 ± 0.8 μg/mL) and TPC (15.2 ± 0.3 mgGAE/grosemary) than the corresponding extract without the enzyme pretreatment.

scholarly journals Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1839
Author(s):  
Harlinda Kuspradini ◽  
Indah Wulandari ◽  
Agmi Sinta Putri ◽  
Sabeti Yulis Tiya ◽  
Irawan Wijaya Kusuma
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
Phytochemical Analysis ◽  
Dpph Radical ◽  
Free Radical Formation ◽  
Dpph Radical Scavenging ◽  
Phytochemical Constituent ◽  
Microdilution Broth

Background: Litsea angulata is a plant species belonging to Lauraceae family that is distributed throughout Indonesia, Malaysia, and New Guinea. The seeds have been traditionally used by local people in Kalimantan, Indonesia for the treatment of boils; however, there is no information about the potency of its branch, bark and leaves yet. This study aimed to determine the antioxidant, antimicrobial activity as well as the phytochemical constituent of Litsea angulata branch, bark, and leaves. Methods: Extraction was performed by successive maceration method using n-hexane, ethyl acetate, and ethanol solvent. Antioxidant activity was evaluated by DPPH radical scavenging assay. The antimicrobial activity using the 96 well-plate microdilution broth method against Staphylococcus aureus and Streptococcus mutans. Results: Based on the phytochemical analysis, it showed that extract of L. angulata contains alkaloids, flavonoids, tannins, terpenoids, and coumarin. The results showed that all extracts of plant samples displayed the ability to inhibit DPPH free radical formation and all tested microorganisms. Conclusions: L. angulata contains secondary metabolites such as alkaloids, flavonoids, tannins, terpenoids, carotenoids, and coumarin. The antioxidant activity on different plant extracts was a range as very strong to weak capacity. All extracts in this study could inhibit the growth of S. aureus and S. mutans.

scholarly journals Antiglycation and Antioxidant Properties of Ficus deltoidea Varieties

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Nur Sumirah Mohd Dom ◽  
Nurshieren Yahaya ◽  
Zainah Adam ◽  
Nik Mohd Afizan Nik Abd. Rahman ◽  
Muhajir Hamid
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protein Carbonyl ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Ficus Deltoidea ◽  
Dpph Radical Scavenging

The present study aimed to evaluate the potential of standardized methanolic extracts from seven Ficus deltoidea varieties in inhibiting the formation of AGEs, protein oxidation, and their antioxidant effects. The antiglycation activity was analyzed based on the inhibition of AGEs, fructosamine, and thiol groups level followed by the inhibition of protein carbonyl formation. The antioxidant activity (DPPH radical scavenging activity and reducing power assay) and total phenolic contents were evaluated. After 28 days of induction, all varieties of Ficus deltoidea extracts significantly restrained the formation of fluorescence AGEs by 4.55–5.14 fold. The extracts also reduced the fructosamine levels by 47.0–86.5%, increased the thiol group levels by 64.3–83.7%, and inhibited the formation of protein carbonyl by 1.36–1.76 fold. DPPH radical scavenging activity showed an IC50 value of 66.81–288.04 μg/ml and reducing power activity depicted at 0.02–0.24 μg/ml. The extent of phenolic compounds present in the extracts ranged from 70.90 to 299.78 mg·GAE/g. Apart from that, correlation studies between the activities were observed. This study revealed that seven varieties of Ficus deltoidea have the potential to inhibit AGEs formation and possess antioxidant activity that might be attributed to the presence of phenolic compounds.

DPPH Radical Scavenging Activity of Kaempferol

2013 ◽  
Vol 781-784 ◽  
pp. 1294-1297 ◽  
Author(s):  
Ping Liu ◽  
Yun Tao Gao ◽  
Jiao Jiao Yu ◽  
Jia Wei Cha ◽  
Dong Mei Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Absorption Spectrum ◽  
Reaction Time ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

The UV-Vis absorption spectrum of kaempferol and DPPH was investigated, the optimum determination wavelength and reaction time for determining the DPPH radical scavenging activity of kaempferol was 517 nm and 30 min, respectively. Kaempferol exhibited strong DPPH radical scavenging activity with a IC50 value of 0.004349 mg·mL-1, which is smaller than that of rutin, indicating that kaempferol has a stronger antioxidant activity than rutin.

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