scholarly journals Enzymatic Hydrolysis Characteristics of Pretreated Rice Straw By Aqueous Ammonia for Bioethanol Production

2011 ◽  
Vol 49 (4) ◽  
pp. 470-474 ◽  
Author(s):  
Yong Cheol Park ◽  
Jun Seok Kim
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Aqueous Ammonia ◽  
Bioethanol Production

Optimization of sodium sulfide treatment of rice straw to increase the enzymatic hydrolysis in bioethanol production

2017 ◽  
Vol 19 (5) ◽  
pp. 1313-1322 ◽  
Author(s):  
Nguyen Thi Minh Phuong ◽  
Phan Huy Hoang ◽  
Le Quang Dien ◽  
Doan Thai Hoa
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Sodium Sulfide ◽  
Bioethanol Production

Box-Behnken design for the optimization of bioethanol production from rice straw and sugarcane bagasse by newly isolated Pichia occidentalis strain AS.2

2021 ◽  
pp. 0958305X2110450
Author(s):  
Ahmed K. Saleh ◽  
Yasser R. Abdel-Fattah ◽  
Nadia A. Soliman ◽  
Maha M. Ibrahim ◽  
Mohamed H. El-Sayed ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Sugarcane Bagasse ◽  
Lignin Content ◽  
Inoculum Size ◽  
Hot Water ◽  
Bioethanol Production ◽  
Box Behnken Design ◽  
Pretreatment Conditions ◽  
Hydrolysis Of

This study investigated bioethanol production from rice straw (RS) and sugarcane bagasse (SCB) which containing 72.8 and 73.2% holocellulose, 56.8 and 58.6% α-cellulose, and 14.9 and 25.1% lignin for RS and SCB, respectively. To eliminate the lignin content, different pretreatment conditions, such as hot water, dilute acid, and acid-alkali, were designed. Acid-alkali was characterized as the best pretreatment for removing ∼79 and 70% of lignin, α-cellulose increased 91.4 and 91%, and holocellulose reached 90.8 and 90% for RS and SCB, respectively. The results revealed that acid-alkali was highly efficient than other pretreatment used for both RS and SCB. After enzymatic hydrolysis of acid-alkali-treated RS and SCB with cellulase, glucose concentrations reached 45 and 42 g/l, respectively. Pichia occidentalis AS.2 was isolated and identified based on 18S rRNA sequencing as a bioethanol producer. Maximization of bioethanol production by P. occidentalis AS.2 using the resulting glucose as a carbon source from RS and SCB was studied using an experimental design. The pH, incubation period, and inoculum size were optimized using Box-Behnken designs (BBD), the final conditions for bioethanol production used 100 g/l acid-alkali-treated fibers, 10 ml cellulase enzyme at 50°C for 5 days at 75 rpm for enzymatic hydrolysis. After time consumed and adjusting the pH to 6, the mixture was inoculated with 2.5% P. occidentalis AS.2 and incubated at 35°C for 24 h at 200 rpm to increase the bioethanol yield by 1.39-fold to 23.7 and 21.4 g/l compared to initial production (17 and 15.3 g/l) between RS and SCB, respectively.

scholarly journals Alkali Pretreatment of Rice Straw to Enhance Enzymatic Hydrolysis for Bioethanol Production

2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Wendy Mateo ◽  
Victorino Taylan ◽  
Perla Florendo ◽  
Rosalie Rafael ◽  
Emmanuel V. Sicat
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Bioethanol Production ◽  
Alkali Pretreatment

Bioethanol production from pretreated whole slurry rice straw by thermophilic co-culture

Fuel ◽  
2021 ◽  
Vol 301 ◽  
pp. 121074
Author(s):  
Nisha Singh ◽  
Ravi P. Gupta ◽  
Suresh K. Puri ◽  
Anshu S. Mathur
Keyword(s):  
Rice Straw ◽  
Bioethanol Production ◽  
Whole Slurry

scholarly journals Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chiranjeevi Thulluri ◽  
Ravi Balasubramaniam ◽  
Harshad Ravindra Velankar
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Deep Eutectic Solvent ◽  
Solvent System ◽  
Cyclic Ether ◽  
Cellulolytic Enzymes ◽  
Ammonium Bromide ◽  
Selective Delignification ◽  
Cellulose Iβ ◽  
Hydrolysis Of

AbstractCellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.

Enzymatic Hydrolysis of Ammonia-Treated Rice Straw

2003 ◽  
Vol 105 (1-3) ◽  
pp. 155-164 ◽  
Author(s):  
Betzabé Sulbarán-De-Ferrer ◽  
Marielena Aristiguieta ◽  
Bruce E. Dale ◽  
Alexis Ferrer ◽  
Graciela Ojeda-De-Rodriguez
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Hydrolysis Of
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