Effect of Acid Pretreatment on Enzymatic Hydrolysis in Bioethanol Production from Rice Straw

2015 ◽  
Vol 6 (1) ◽  
pp. 3 ◽  
Author(s):  
H. B. Aditiya ◽  
K.P. Sing ◽  
M. Hanif ◽  
T.M.I. Mahlia
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Bioethanol Production ◽  
Acid Pretreatment

scholarly journals CELLULOSIC BIOETHANOL PRODUCTION FROM ULVA LACTUCA MACROALGAE

2021 ◽  
Vol 55 (5-6) ◽  
pp. 629-635
Author(s):  
AMINA ALLOUACHE ◽  
AZIZA MAJDA ◽  
AHMED ZAID TOUDERT ◽  
ABDELTIF AMRANE ◽  
MERCEDES BALLESTEROS
Keyword(s):  
Enzymatic Hydrolysis ◽  
Fossil Fuels ◽  
Ethanol Yield ◽  
Lignin Content ◽  
Arable Land ◽  
Ulva Lactuca ◽  
Marine Macroalgae ◽  
Bioethanol Production ◽  
Acid Pretreatment ◽  
Total Glucose

Nowadays, the use of biofuels has become an unavoidable solution to the depletion of fossil fuels and global warming. The controversy over the use of food crops for the production of the first-generation biofuels and deforestation caused by the second-generation ones has forced the transition to the third generation of biofuels, which avoids the use of arable land and edible products, and does not threaten biodiversity. This generation is based on the marine and freshwater biomass, which has the advantages of being abundant or even invasive, easy to cultivate and having a good energetic potential. Bioethanol production from Ulva lactuca, a local marine macroalgae collected from the west coast of Algiers, was examined in this study. Ulva lactuca showed a good energetic potential due to its carbohydrate-rich content: 9.57% of cellulose, 6.9% of hemicellulose and low lignin content of 5.11%. Ethanol was produced following the separate hydrolysis and fermentation process (SHF), preceded by a thermal acid pretreatment at 120 °C during 15 min. Enzymatic hydrolysis was performed using a commercial cellulase (Celluclast 1.5 L), which saccharified the cellulose contained in the green seaweed, releasing about 85.01% of the total glucose, corresponding to 7.21 g/L after 96 h of enzymatic hydrolysis at pH 5 and 45 °C. About 3.52 g/L of ethanol was produced after 48 h of fermentation using Saccharomyces cerevisiae at 30 °C and pH 5, leading to a high ethanol yield of 0.41 g of ethanol/g of glucose.

Optimization of sodium sulfide treatment of rice straw to increase the enzymatic hydrolysis in bioethanol production

2017 ◽  
Vol 19 (5) ◽  
pp. 1313-1322 ◽  
Author(s):  
Nguyen Thi Minh Phuong ◽  
Phan Huy Hoang ◽  
Le Quang Dien ◽  
Doan Thai Hoa
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Sodium Sulfide ◽  
Bioethanol Production

Effect of dilute acid pretreatment of rice straw on structural properties and enzymatic hydrolysis

2010 ◽  
Vol 101 (13) ◽  
pp. 4907-4913 ◽  
Author(s):  
Teng-Chieh Hsu ◽  
Gia-Luen Guo ◽  
Wen-Hua Chen ◽  
Wen-Song Hwang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Structural Properties ◽  
Rice Straw ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Acid Pretreatment

Box-Behnken design for the optimization of bioethanol production from rice straw and sugarcane bagasse by newly isolated Pichia occidentalis strain AS.2

2021 ◽  
pp. 0958305X2110450
Author(s):  
Ahmed K. Saleh ◽  
Yasser R. Abdel-Fattah ◽  
Nadia A. Soliman ◽  
Maha M. Ibrahim ◽  
Mohamed H. El-Sayed ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Sugarcane Bagasse ◽  
Lignin Content ◽  
Inoculum Size ◽  
Hot Water ◽  
Bioethanol Production ◽  
Box Behnken Design ◽  
Pretreatment Conditions ◽  
Hydrolysis Of

This study investigated bioethanol production from rice straw (RS) and sugarcane bagasse (SCB) which containing 72.8 and 73.2% holocellulose, 56.8 and 58.6% α-cellulose, and 14.9 and 25.1% lignin for RS and SCB, respectively. To eliminate the lignin content, different pretreatment conditions, such as hot water, dilute acid, and acid-alkali, were designed. Acid-alkali was characterized as the best pretreatment for removing ∼79 and 70% of lignin, α-cellulose increased 91.4 and 91%, and holocellulose reached 90.8 and 90% for RS and SCB, respectively. The results revealed that acid-alkali was highly efficient than other pretreatment used for both RS and SCB. After enzymatic hydrolysis of acid-alkali-treated RS and SCB with cellulase, glucose concentrations reached 45 and 42 g/l, respectively. Pichia occidentalis AS.2 was isolated and identified based on 18S rRNA sequencing as a bioethanol producer. Maximization of bioethanol production by P. occidentalis AS.2 using the resulting glucose as a carbon source from RS and SCB was studied using an experimental design. The pH, incubation period, and inoculum size were optimized using Box-Behnken designs (BBD), the final conditions for bioethanol production used 100 g/l acid-alkali-treated fibers, 10 ml cellulase enzyme at 50°C for 5 days at 75 rpm for enzymatic hydrolysis. After time consumed and adjusting the pH to 6, the mixture was inoculated with 2.5% P. occidentalis AS.2 and incubated at 35°C for 24 h at 200 rpm to increase the bioethanol yield by 1.39-fold to 23.7 and 21.4 g/l compared to initial production (17 and 15.3 g/l) between RS and SCB, respectively.

scholarly journals Alkali Pretreatment of Rice Straw to Enhance Enzymatic Hydrolysis for Bioethanol Production

2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Wendy Mateo ◽  
Victorino Taylan ◽  
Perla Florendo ◽  
Rosalie Rafael ◽  
Emmanuel V. Sicat
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Bioethanol Production ◽  
Alkali Pretreatment
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