scholarly journals Effects of Methyl Jasmonate and Salicylic Acid on Alkaloid Production from in vitro Culture of Stemona sp.

Author(s):  
Natthiya Chaichana ◽  
Srisulak Dheeranupattana
2021 ◽  
Vol 923 (1) ◽  
pp. 012010
Author(s):  
Ekhlas Meteab Ahmed Marir

Abstract This experiment was conducted in the Plant Tissue Culture Laboratory, College of Agricultural Engineering Sciences, University of Baghdad for the period from September/2018 to July 2019. The induced callus from the cotyledon leaves of seedlings of the Spilanthes acmella plant was used in order to know the effect of chemical catalysts and Starmedium was added to Glutamine (250, 300, 350) mgters on the chemical content. After 4 weeks of planting, the primary callus was planted at 150 mg in the nutrient medium supplemented with auxin,4-dichlorophenoxy acetic acid (2,4-D) 2.0 mg.L−1 and cytokinin Benzyl Adenine (BA) 0.5 mg.L−1 at constant concentrations in the first five medium, to which the catalyst was added salicylic acid at concentrations (25, 50, 75) μmol). The second medium was added to methyl jasmonate at concentrations (25, 50, 75 μmol) of the third medium was added to Casein hydrolysate at concentrations (25, 50, 75 μmol) of the fourth medium was added to Glutamine (250, 300, 350) mg. L−1. The results showed that the treatment of nutritional medium with high concentrations of stimulants and primer led to a significant increase in the content of plant tissues (the induced callus from the cotyledons) of total carbohydrates, the percentage of protein, the content of callus from the carotene pigment and content of proline, while the comparison treatment was the most effective in vegetable tissue contents of total carbohydrates and protein percentage and content of callus from the carotene pigment and proline, as well as this confirms that all treatments led to a positive and direct increase of chemical compounds content of plant tissues of chemical traits, especially in the treatment of Salicylic acid, methyl jasmonate, casein hydrolysate, glutamine, and phenylalanine (75 micromoles, 75 micromoles, 75 micromoles, 350 mg.L−1, 150 micromoles) respectively, were followed by the treatments of Salicylic acid, methyl jasmonate, casein hydrolysate, glutamine and phenylalanine (50 μmol, 50 μmol, 50 μmol, 300 mg.L−1, 100 μmol), respectively. The aim of this study is to know the effect of Salicylic acid, methyl jasmonate, casein hydrolysate, glutamine, and phenylalanine in the induction and differentiation of callus of cotyledon leaves cotyledon leaves of Spilanthes acmella seedlings on some chemical traits in vitro.


2020 ◽  
Vol 10 (24) ◽  
pp. 8916
Author(s):  
Risa Yamamoto ◽  
Gang Ma ◽  
Lancui Zhang ◽  
Miki Hirai ◽  
Masaki Yahata ◽  
...  

Salicylic acid and jasmonic acid are two important plant hormones that trigger the plant defense responses and regulate the accumulation of bioactive compounds in plants. In the present study, the effects of salicylic acid (SA) and methyl jasmonate (MeJA) on flavonoid and carotenoid accumulation were investigated in the juice sacs of Satsuma mandarin in vitro. The results showed that SA treatment was effective to enhance the contents of eriocitrin, narirutin, poncirin, and β-cryptoxanthin in the juice sacs (p < 0.05). In contrast, the MeJA treatment inhibited flavonoid and carotenoid accumulation in the juice sacs (p < 0.05). Gene expression results showed that the changes of flavonoid and carotenoid contents in the SA and MeJA treatments were highly regulated at the transcriptional level. In addition, a transcriptional factor CitWRKY70 was identified in the microarray analysis, which was induced by the SA treatment, while suppressed by the MeJA treatment. In the SA and MeJA treatments, the change in the expression of CitWRKY70 was consistent with that of flavonoid and carotenoid biosynthetic key genes. These results indicated that CitWRKY70 might be involved in the regulation of flavonoid and carotenoid accumulation in response to SA and MeJA treatments in the juice sacs of citrus fruit.


Sugar Tech ◽  
2019 ◽  
Vol 21 (6) ◽  
pp. 1031-1038 ◽  
Author(s):  
Sajjad Moharramnejad ◽  
Amirali Taheri Azam ◽  
Jaber Panahandeh ◽  
Zahra Dehghanian ◽  
Muhammad Ashraf

2021 ◽  
Vol 43 ◽  
pp. 60-64
Author(s):  
Aimie Nadzirah Abd Malik ◽  
Jasim Uddain ◽  
Chee Keong Chin ◽  
Bee Lynn Chew ◽  
Sreeramanan Subramaniam

Sign in / Sign up

Export Citation Format

Share Document