scholarly journals Effects of Salicylic Acid and Methyl Jasmonate Treatments on Flavonoid and Carotenoid Accumulation in the Juice Sacs of Satsuma Mandarin In Vitro

2020 ◽  
Vol 10 (24) ◽  
pp. 8916
Author(s):  
Risa Yamamoto ◽  
Gang Ma ◽  
Lancui Zhang ◽  
Miki Hirai ◽  
Masaki Yahata ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Methyl Jasmonate ◽  
Citrus Fruit ◽  
Defense Responses ◽  
Transcriptional Level ◽  
Carotenoid Accumulation ◽  
Satsuma Mandarin ◽  
Meja Treatment ◽  
Juice Sacs

Salicylic acid and jasmonic acid are two important plant hormones that trigger the plant defense responses and regulate the accumulation of bioactive compounds in plants. In the present study, the effects of salicylic acid (SA) and methyl jasmonate (MeJA) on flavonoid and carotenoid accumulation were investigated in the juice sacs of Satsuma mandarin in vitro. The results showed that SA treatment was effective to enhance the contents of eriocitrin, narirutin, poncirin, and β-cryptoxanthin in the juice sacs (p < 0.05). In contrast, the MeJA treatment inhibited flavonoid and carotenoid accumulation in the juice sacs (p < 0.05). Gene expression results showed that the changes of flavonoid and carotenoid contents in the SA and MeJA treatments were highly regulated at the transcriptional level. In addition, a transcriptional factor CitWRKY70 was identified in the microarray analysis, which was induced by the SA treatment, while suppressed by the MeJA treatment. In the SA and MeJA treatments, the change in the expression of CitWRKY70 was consistent with that of flavonoid and carotenoid biosynthetic key genes. These results indicated that CitWRKY70 might be involved in the regulation of flavonoid and carotenoid accumulation in response to SA and MeJA treatments in the juice sacs of citrus fruit.

scholarly journals Expression and functional analysis of the nobiletin biosynthesis-related gene CitOMT in citrus fruit

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mao Seoka ◽  
Gang Ma ◽  
Lancui Zhang ◽  
Masaki Yahata ◽  
Kazuki Yamawaki ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Citrus Fruit ◽  
Satsuma Mandarin ◽  
Important Health ◽  
Nutritional Qualities ◽  
Citrus Varieties ◽  
Ponkan Mandarin ◽  
Methylation Activity ◽  
New Strategies

Abstract Nobiletin, a polymethoxy flavone (PMF), is specific to citrus and has been reported to exhibit important health-supporting properties. Nobiletin has six methoxy groups at the 3′,4′,5,6,7,8-positions, which are catalyzed by O-methyltransferases (OMTs). To date, researches on OMTs in citrus fruit are still limited. In the present study, a novel OMT gene (CitOMT) was isolated from two citrus varieties Satsuma mandarin (Citrus unshiu Marc.) and Ponkan mandarin (Citrus reticulata Blanco), and its function was characterized in vitro. The results showed that the expression of CitOMT in the flavedo of Ponkan mandarin was much higher than that of Satsuma mandarin during maturation, which was consistent with the higher accumulation of nobiletin in Ponkan mandarin. In addition, functional analysis showed that the recombinant protein of CitOMT had methylation activity to transfer a methyl group to 3′-hydroxy group of flavones in vitro. Because methylation at the 3′-position of flavones is vital for the nobiletin biosynthesis, CitOMT may be a key gene responsible for nobiletin biosynthesis in citrus fruit. The results presented in this study will provide new strategies to enhance nobiletin accumulation and improve the nutritional qualities of citrus fruit.

scholarly journals Priming of Defense Systems and Upregulation of MYC2 and JAZ1 Genes after Botrytis cinerea Inoculation in Methyl Jasmonate-Treated Strawberry Fruits

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 447 ◽  
Author(s):  
Felipe Valenzuela-Riffo ◽  
Paz E. Zúñiga ◽  
Luis Morales-Quintana ◽  
Mauricio Lolas ◽  
Marcela Cáceres ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Botrytis Cinerea ◽  
Defense Responses ◽  
Gray Mold ◽  
The Past ◽  
Defense Systems ◽  
Meja Treatment ◽  
Fruit Development And Ripening ◽  
Genes Encoding ◽  
And Control

Several attempts have been made to study the effects of methyl jasmonate (MeJA) on plants in the past years. However, the comparative effects of the number and phenological time of MeJA applications on the activation of defense systems is currently unknown in strawberries. In the present research, we performed three field treatments during strawberry (Fragaria × ananassa ‘Camarosa’) fruit development and ripening which consisted of differential MeJA applications at flowering (M3), and the large green (M2 and M3) and red ripe (M1, M2, and M3) fruit stages. We also checked changes in gene expression related to plant defense against Botrytis cinerea inoculation post-harvest. In M3 treatment, we observed an upregulation of the anthocyanin and lignin contents and the defense-related genes, encoding for chitinases, β-1,3-glucanases and polygalacturonase-inhibiting proteins, after harvest (0 hpi), along with the jasmonate signaling-related genes FaMYC2 and FaJAZ1 at 48 h after B. cinerea inoculation (48 hpi) during postharvest storage. Although we did not find differences in gray mold incidence between the MeJA treatments and control, these results suggest that preharvest MeJA treatment from the flowering stage onwards (M3) primes defense responses mediated by the upregulation of different defense-related genes and retains the upregulation of MYC2 and JAZ1 at 48 hpi.

scholarly journals Effect of Different Levels of Catalysts and Chemicals Primer on Content of Induced Callus Tissue of Cotyledon Leaves of Spilanthes Acmella Seedlings on Some Chemical Traits in Vitro

2021 ◽  
Vol 923 (1) ◽  
pp. 012010
Author(s):  
Ekhlas Meteab Ahmed Marir
Keyword(s):  
Salicylic Acid ◽  
Methyl Jasmonate ◽  
Casein Hydrolysate ◽  
Plant Tissues ◽  
Acid Methyl ◽  
Engineering Sciences ◽  
Spilanthes Acmella ◽  
Total Carbohydrates ◽  
Chemical Traits

Abstract This experiment was conducted in the Plant Tissue Culture Laboratory, College of Agricultural Engineering Sciences, University of Baghdad for the period from September/2018 to July 2019. The induced callus from the cotyledon leaves of seedlings of the Spilanthes acmella plant was used in order to know the effect of chemical catalysts and Starmedium was added to Glutamine (250, 300, 350) mgters on the chemical content. After 4 weeks of planting, the primary callus was planted at 150 mg in the nutrient medium supplemented with auxin,4-dichlorophenoxy acetic acid (2,4-D) 2.0 mg.L−1 and cytokinin Benzyl Adenine (BA) 0.5 mg.L−1 at constant concentrations in the first five medium, to which the catalyst was added salicylic acid at concentrations (25, 50, 75) μmol). The second medium was added to methyl jasmonate at concentrations (25, 50, 75 μmol) of the third medium was added to Casein hydrolysate at concentrations (25, 50, 75 μmol) of the fourth medium was added to Glutamine (250, 300, 350) mg. L−1. The results showed that the treatment of nutritional medium with high concentrations of stimulants and primer led to a significant increase in the content of plant tissues (the induced callus from the cotyledons) of total carbohydrates, the percentage of protein, the content of callus from the carotene pigment and content of proline, while the comparison treatment was the most effective in vegetable tissue contents of total carbohydrates and protein percentage and content of callus from the carotene pigment and proline, as well as this confirms that all treatments led to a positive and direct increase of chemical compounds content of plant tissues of chemical traits, especially in the treatment of Salicylic acid, methyl jasmonate, casein hydrolysate, glutamine, and phenylalanine (75 micromoles, 75 micromoles, 75 micromoles, 350 mg.L−1, 150 micromoles) respectively, were followed by the treatments of Salicylic acid, methyl jasmonate, casein hydrolysate, glutamine and phenylalanine (50 μmol, 50 μmol, 50 μmol, 300 mg.L−1, 100 μmol), respectively. The aim of this study is to know the effect of Salicylic acid, methyl jasmonate, casein hydrolysate, glutamine, and phenylalanine in the induction and differentiation of callus of cotyledon leaves cotyledon leaves of Spilanthes acmella seedlings on some chemical traits in vitro.

Effects of postharvest methyl jasmonate treatments against Botrytis cinerea on Geraldton waxflower (Chamelaucium uncinatum)

2006 ◽  
Vol 46 (5) ◽  
pp. 717 ◽  
Author(s):  
J. X. Eyre ◽  
J. Faragher ◽  
D. C. Joyce ◽  
P. R. Franz
Keyword(s):  
Methyl Jasmonate ◽  
Botrytis Cinerea ◽  
Induced Systemic Resistance ◽  
Floral Organ ◽  
Ethylene Biosynthesis ◽  
Systemic Resistance ◽  
Meja Treatment ◽  
Flower Abscission ◽  
Chamelaucium Uncinatum

Cut Geraldton waxflower (Chamelaucium uncinatum Schauer) flowers are often infected with Botrytis cinerea. Release of infection from quiescence can cause ethylene production by invaded host tissues and result in flower abscission. Postharvest floral organ abscission is a major problem for the commercial waxflower industry. Methyl jasmonate (MeJA) occurs naturally in plant tissue and has a signalling role in eliciting induced systemic resistance against disease. MeJA treatments have been shown to suppress B. cinerea infecting cut rose flowers. The present experiments investigated the potential of exogenous MeJA treatments for B. cinerea management on harvested waxflower. MeJA treatments of 10 and 100 µL liquid MeJA/L of air applied to cv. Purple Pride and 1 µL MeJA/L to cv. Mullering Brook gave reductions in disease severity for uninoculated stems. However, concentrations of 100 µL MeJA/L applied to Purple Pride in addition to 1 and 10 µL MeJA/L applied to Mullering Brook increased the incidence of floral organ fall. Flower abscission upon treatment with MeJA may be due to induced systemic resistance-associated upregulation of ethylene biosynthesis. MeJA treatments had no direct effect on B. cinerea hyphal elongation in vitro. Collectively, these results show that while MeJA treatment may elicit defence in waxflower against Botrytis, the chemical also causes floral organ fall. Thus, exogenous MeJA treatments do not have potential for B. cinerea management on harvested waxflower.

scholarly journals Exogenous Application of ABA and NAA Alleviates the Delayed Coloring Caused by Puffing Inhibitor in Citrus Fruit

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 308
Author(s):  
Gang Ma ◽  
Lancui Zhang ◽  
Rin Kudaka ◽  
Hayato Inaba ◽  
Takuma Furuya ◽  
...  
Keyword(s):  
Combined Treatment ◽  
Citrus Fruit ◽  
Fruit Color ◽  
Naphthaleneacetic Acid ◽  
Transcriptional Level ◽  
Ripening Process ◽  
Carotenoid Accumulation ◽  
Exogenous Application ◽  
Color Changes ◽  
Color Development

Combined spraying of gibberellin (GA) and prohydrojasmon (PDJ) was an effective method to reduce peel puffing in Satsuma mandarins. However, in the GA-and-PDJ combined treatment, fruit color development was delayed during the ripening process. In the present study, to improve the coloration of the GA and PDJ-treated fruit, the effects of exogenous application of 1-naphthaleneacetic acid (NAA) and abscisic acid (ABA) on chlorophyll and carotenoid accumulation were investigated. The results showed that both ABA and NAA treatments accelerated the color changes from green to orange in the GA and PDJ-treated fruit during the ripening process. With the NAA and ABA treatments, chlorophylls contents were decreased rapidly, and the contents of β,β-xanthophylls were significantly enhanced in the GA and PDJ-treated fruit. In addition, gene expression results showed that the changes of the chlorophyll and carotenoid metabolisms in the NAA and ABA treatments were highly regulated at the transcriptional level. The results presented in this study suggested that the application of NAA and ABA could potentially be used for improving the coloration of the GA and PDJ-treated fruit.

Effect of Methyl Jasmonate and Salicylic Acid on In Vitro Growth, Stevioside Production, and Oxidative Defense System in Stevia rebaudiana

Sugar Tech ◽  
2019 ◽  
Vol 21 (6) ◽  
pp. 1031-1038 ◽  
Author(s):  
Sajjad Moharramnejad ◽  
Amirali Taheri Azam ◽  
Jaber Panahandeh ◽  
Zahra Dehghanian ◽  
Muhammad Ashraf
Keyword(s):  
Salicylic Acid ◽  
Methyl Jasmonate ◽  
Stevia Rebaudiana ◽  
In Vitro Growth ◽  
Defense System ◽  
Oxidative Defense

Effects of Methyl Jasmonate on Cylindrocarpon Root Rot Development on Ginseng

2012 ◽  
Vol 610-613 ◽  
pp. 3511-3517 ◽  
Author(s):  
Jia Man Sun ◽  
Jun Fan Fu ◽  
Ru Jun Zhou ◽  
Wei Na Su ◽  
Xue Rui Yan
Keyword(s):  
Methyl Jasmonate ◽  
Disease Severity ◽  
Root Rot ◽  
Severe Disease ◽  
Defense Responses ◽  
Defense System ◽  
Colony Diameter ◽  
Low Concentrations ◽  
Mda Content

Cylindrocarpon root rot, caused by Cylindrocarpon destructans, is a severe disease on ginseng (Panax ginseng C.A. Meyer) in northeast China. Methyl jasmonate (MeJA) has been studied for its ability to induce plant defenses against pathogens. The effects of MeJA on the colony diameter of C. destructans and seedling growth were examined in vitro. Two-year-old ginseng roots were drenched with MeJA (200 mg/L) and then inoculated with conidia suspension of C. destructans (1×106 conidia/ml). Disease severity was assessed on inoculated roots, and the activation of defense responses was also measured. Results showed that MeJA had no effect on the growth of C. destructans and seedlings at low concentrations. The incidence rate and disease severity on MeJA-treated ginseng roots was significantly reduced compared with untreated roots. The proline content was increased but the malondialdehyde (MDA) content decreased in MeJA- treated roots. The activities of phenylalanine amino-lyase (PAL), catalase (CAT), peroxidase (POD) and polyphenoloxidase (PPO) were significantly enhanced in treated roots. It was concluded that low concentrations of MeJA had no effect on C. destructans, but it stimulated the activities of defense enzymes in treated ginseng roots and alleviated the damage of roots from C. destructans. Therefore, the control of Cylindrocarpon root rot by MeJA may involve the activation of the disease-related defense system.

Elicitation of protocorm-like bodies (PLBs) of Dendrobium ‘Sabin Blue’ using methyl jasmonate, salicylic acid and melatonin for in vitro production of anthocyanin

2021 ◽  
Vol 43 ◽  
pp. 60-64
Author(s):  
Aimie Nadzirah Abd Malik ◽  
Jasim Uddain ◽  
Chee Keong Chin ◽  
Bee Lynn Chew ◽  
Sreeramanan Subramaniam
Keyword(s):  
Salicylic Acid ◽  
Methyl Jasmonate ◽  
In Vitro Production ◽  
Vitro Production

Effect of Postharvest Temperature and Ethylene on Carotenoid Accumulation in the Flavedo and Juice Sacs of Satsuma Mandarin (Citrus unshiu Marc.) Fruit

2009 ◽  
Vol 57 (11) ◽  
pp. 4724-4732 ◽  
Author(s):  
Hikaru Matsumoto ◽  
Yoshinori Ikoma ◽  
Masaya Kato ◽  
Naoko Nakajima ◽  
Yoshinori Hasegawa
Keyword(s):  
Citrus Unshiu ◽  
Carotenoid Accumulation ◽  
Satsuma Mandarin ◽  
Juice Sacs
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