Fungal Viridionychia: Onychomycosis-Induced Chloronychia Caused by Candida parapsilosis-Associated Green Nail Discoloration

Copper(II) and zinc(II) complexes with clinically used antifungal drug fluconazole (fcz), {[CuCl2(fcz)2].5H2O}n, 1, and {[ZnCl2(fcz)2]·2C2H5OH}n, 2, were prepared and characterized by spectroscopic and crystallographic methods. The polymeric structure of the complexes comprises four fluconazole molecules monodentately coordinated via the triazole nitrogen and two chlorido ligands. With respect to fluconazole, complex 2 showed significantly higher antifungal activity against Candida krusei and Candida parapsilosis. All tested compounds reduced the total amount of ergosterol at subinhibitory concentrations, indicating that the mode of activity of fluconazole was retained within the complexes, which was corroborated via molecular docking with cytochrome P450 sterol 14α-demethylase (CYP51) as a target. Electrostatic, steric and internal energy interactions between the complexes and enzyme showed that 2 has higher binding potency to this target. Both complexes showed strong inhibition of C. albicans filamentation and biofilm formation at subinhibitory concentrations, with 2 being able to reduce the adherence of C. albicans to A549 cells in vitro. Complex 2 was able to reduce pyocyanin production in Pseudomonas aeruginosa between 10% and 25% and to inhibit its biofilm formation by 20% in comparison to the untreated control. These results suggest that complex 2 may be further examined in the mixed Candida-P. aeruginosa infections.

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A case series of medically managed Candida parapsilosis complex prosthetic valve endocarditis

Annals of Clinical Microbiology and Antimicrobials ◽

10.1186/s12941-020-00409-4 ◽

2021 ◽

Vol 20 (1) ◽

Author(s):

Penghao Guo ◽

Yuting He ◽

Rui Fan ◽

Zhongwen Wu ◽

Yili Chen ◽

...

Keyword(s):

Prosthetic Valve ◽

Candida Parapsilosis ◽

Case Series ◽

Prosthetic Valve Endocarditis ◽

Sensu Stricto ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Candida Metapsilosis ◽

Candida Orthopsilosis ◽

Tof Ms

Abstract Background In recent years, Candida parapsilosis is recognized as a species complex and is composed of Candida parapsilosis sensu stricto, Candida orthopsilosis and Candida metapsilosis. Candida parapsilosis complex prosthetic valve endocarditis (PVE) is rare and the survival rate is still low despite of optimal therapeutic strategies. In our report, it is novel to report cases as Candida parapsilosis complex PVE at species and identify Candida parapsilosis using MALDI-TOF MS. Case presentation A series of 4 cases of Candida parapsilosis complex PVE from our institution was reported. Three were infected by Candida parapsilosis sensu stricto and one was infected by Candida metapsilosis. The condition of two cases got better and the other died. Conclusions More attention should be paid to Candida parapsilosis complex PVE and early diagnosis and prompt antibiotic therapy may play a role in the treatment for Candida parapsilosis complex PVE. It is recommended to identify Candida parapsilosis complex at species level and MALDI-TOF MS as an easy, fast and efficient identification method is worth promoting in clinical microbiology

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Deracemisation of β-hydroxy esters using immobilised whole cells of Candida parapsilosis ATCC 7330: substrate specificity and mechanistic investigation

Tetrahedron ◽

10.1016/j.tet.2006.03.045 ◽

2006 ◽

Vol 62 (21) ◽

pp. 5133-5140 ◽

Cited By ~ 45

Author(s):

Santosh Kumar Padhi ◽

D. Titu ◽

N. Ganesh Pandian ◽

Anju Chadha

Keyword(s):

Substrate Specificity ◽

Candida Parapsilosis ◽

Whole Cells ◽

Mechanistic Investigation ◽

Hydroxy Esters ◽

Candida Parapsilosis Atcc 7330

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