scholarly journals An In Vitro Study Measuring the Effects of Circumferential and Near-Circumferential Closed Incisional Negative Pressure Wound Therapy Dressings

Cureus ◽  
2021 ◽  
Author(s):  
John P Livingstone ◽  
Dylan Singh ◽  
Patrick C Murray
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
In Vitro Study ◽  
Vitro Study ◽  
Wound Therapy

Efficacy and Safety of Various Active Irrigation Devices When Used with Either Positive or Negative Pressure: An In Vitro Study

2012 ◽  
Vol 38 (12) ◽  
pp. 1622-1626 ◽  
Author(s):  
Augusto Malentacca ◽  
Umberto Uccioli ◽  
Dario Zangari ◽  
Carlo Lajolo ◽  
Cristiano Fabiani
Keyword(s):  
Negative Pressure ◽  
In Vitro Study ◽  
Vitro Study ◽  
Efficacy And Safety

Potential Danger of Pre-Pump Clamping on Negative Pressure-Associated Gaseous Microemboli Generation During Extracorporeal Life Support-An In Vitro Study

2015 ◽  
Vol 40 (1) ◽  
pp. 89-94 ◽  
Author(s):  
Shigang Wang ◽  
Brian J. Chin ◽  
Frank Gentile ◽  
Allen R. Kunselman ◽  
David Palanzo ◽  
...  
Keyword(s):  
Negative Pressure ◽  
Life Support ◽  
Extracorporeal Life Support ◽  
In Vitro Study ◽  
Vitro Study ◽  
Potential Danger

scholarly journals Characterization of Macrophage and Cytokine Interactions with Biomaterials Used in Negative-Pressure Wound Therapy

2021 ◽  
Vol 9 (1) ◽  
pp. 2
Author(s):  
Praveen Krishna Veerasubramanian ◽  
Victor C. Joe ◽  
Wendy F. Liu ◽  
Timothy L. Downing
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Biological Effects ◽  
Wound Therapy ◽  
Tnf Α ◽  
Macrophage Adhesion ◽  
Cytokine Interactions ◽  
Control Surfaces

Macrophages are innate immune cells that help wounds heal. Here, we study the potential immunomodulatory effects of negative-pressure wound therapy (NPWT) materials on the macrophage inflammatory response. We compared the effects of two materials, Granufoam™ (GF) and Veraflo Cleanse™ (VC), on macrophage function in vitro. We find that both materials cause reduced expression of inflammatory genes, such as TNF and IL1B, in human macrophages stimulated with bacterial lipopolysaccharide (LPS) and interferon-gamma (IFNγ). Relative to adherent glass control surfaces, VC discourages macrophage adhesion and spreading, and may potentially sequester LPS/IFNγ and cytokines that the cells produce. GF, on the other hand, was less suppressive of inflammation, supported macrophage adhesion and spreading better than VC, and sequestered lesser quantities of LPS/IFNγ in comparison to VC. The control dressing material cotton gauze (CT) was also immunosuppressive, capable of TNF-α retention and LPS/IFNγ sequestration. Our findings suggest that NPWT material interactions with cells, as well as soluble factors including cytokines and LPS, can modulate the immune response, independent of vacuum application. We have also established methodological strategies for studying NPWT materials and reveal the potential utility of cell-based in vitro studies for elucidating biological effects of NPWT materials.

The effect of negative pressure wound therapy with antibacterial dressings or antiseptics on an in vitro wound model

2017 ◽  
Vol 26 (5) ◽  
pp. 236-242 ◽  
Author(s):  
J. Matiasek ◽  
K.J. Domig ◽  
G. Djedovic ◽  
R. Babeluk ◽  
O. Assadian
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Wound Therapy ◽  
Wound Model

scholarly journals The Effect of Negative Pressure Wound Therapy with and without Instillation on Mature Biofilms In Vitro

Materials ◽  
2018 ◽  
Vol 11 (5) ◽  
pp. 811 ◽  
Author(s):  
Shamaila Tahir ◽  
Matthew Malone ◽  
Honghua Hu ◽  
Anand Deva ◽  
Karen Vickery
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Wound Therapy

scholarly journals Technical Support of Wound Healing Processes: Project Status

2019 ◽  
Vol 5 (1) ◽  
pp. 521-523
Author(s):  
Jacquelyn Dawn Parente ◽  
Knut Möller ◽  
Sabine Hensler ◽  
Claudia Kühlbach ◽  
Margareta M. Mueller ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mathematical Models ◽  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Technical Support ◽  
Wound Therapy ◽  
Wound Area ◽  
Modal Treatment ◽  
Healing Processes

AbstractThe optimized wound healing (OWID) project provides technical support of wound healing processes. Advanced biophysical treatment therapies using light (photobiomodulation), negative pressure wound therapy (NPWT), and electrical stimulation show biological effects. Specifically, a biphasic dose-response curve is observed where lower doses activate cells, while above a threshold, higher doses are inhibitory. However, no standard protocols and no multi-modal treatment studies determine specific therapy needs. The OWID project aims to develop a multi-modal treatment device and modelbased therapy for individualized wound healing. This work presents the OWID project status. Currently, a photobiomodulation prototype delivers red, green, and blue light ‘medicine’ at prescribed therapeutic ‘doses’. The calculation of incident light necessarily considers transmission properties of the intervening cell culture plate. Negative pressure wound therapy (NPWT) and electrical impedance tomography (EIT) hardware are being adapted for use in vitro. Development of mathematical models of wound healing and therapy control are supported by treatment experiment outcome measures conducted in a wounded 3D tissue model. Parameter sensitivity analysis conducted on an existing mathematical model of reepithelialization results in changing parameter values influencing cellular movement rates. Thus, the model is robust to fit model parameters to observed reepithelialization rates under treatment conditions impacting cellular activation, inhibition, and untreated controls. Developed image analysis techniques have not captured changes in wound area after photobiomodulation treatment experiments. Alternatively, EIT will be tested for wound area analysis. Additionally, live dyes will be introduced to non-invasively visualize the reepithelialization front on a smaller, cellular scale. Finally, an overall therapeutic feedback control model uses model reference adaptive control to incorporate the intrinsic biological reepithelialization mechanism, treatment loops, and treatment controller modulation at a wound state. Currently, the OWID project conducts photobiomodulation treatment experiments in vitro and has developed mathematical models. Future work includes the incorporation of multi-modal wound healing treatment experiments.

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