Application of a drainage film reduces fibroblast ingrowth into large-pored polyurethane foam during negative-pressure wound therapy in an in vitro model

2013 ◽  
Vol 21 (5) ◽  
pp. 697-703 ◽  
Author(s):  
Cornelia Wiegand ◽  
Steffen Springer ◽  
Martin Abel ◽  
Falko Wesarg ◽  
Peter Ruth ◽  
...  
Keyword(s):  
Polyurethane Foam ◽  
Negative Pressure ◽  
In Vitro Model ◽  
Negative Pressure Wound Therapy ◽  
Wound Therapy ◽  
Vitro Model

scholarly journals Polyurethane foam scaffolds: a novel 3D in vitro model for breast cancer-derived bone metastasis

2016 ◽  
Vol 4 ◽  
Author(s):  
Contessi Nicola ◽  
Angeloni Valentina ◽  
De Marco Cinzia ◽  
Tanzi Maria Cristina ◽  
Daidone Maria Grazia ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Metastasis ◽  
Polyurethane Foam ◽  
In Vitro Model ◽  
3D In Vitro Model ◽  
Vitro Model

scholarly journals Characterization of Macrophage and Cytokine Interactions with Biomaterials Used in Negative-Pressure Wound Therapy

2021 ◽  
Vol 9 (1) ◽  
pp. 2
Author(s):  
Praveen Krishna Veerasubramanian ◽  
Victor C. Joe ◽  
Wendy F. Liu ◽  
Timothy L. Downing
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Biological Effects ◽  
Wound Therapy ◽  
Tnf Α ◽  
Macrophage Adhesion ◽  
Cytokine Interactions ◽  
Control Surfaces

Macrophages are innate immune cells that help wounds heal. Here, we study the potential immunomodulatory effects of negative-pressure wound therapy (NPWT) materials on the macrophage inflammatory response. We compared the effects of two materials, Granufoam™ (GF) and Veraflo Cleanse™ (VC), on macrophage function in vitro. We find that both materials cause reduced expression of inflammatory genes, such as TNF and IL1B, in human macrophages stimulated with bacterial lipopolysaccharide (LPS) and interferon-gamma (IFNγ). Relative to adherent glass control surfaces, VC discourages macrophage adhesion and spreading, and may potentially sequester LPS/IFNγ and cytokines that the cells produce. GF, on the other hand, was less suppressive of inflammation, supported macrophage adhesion and spreading better than VC, and sequestered lesser quantities of LPS/IFNγ in comparison to VC. The control dressing material cotton gauze (CT) was also immunosuppressive, capable of TNF-α retention and LPS/IFNγ sequestration. Our findings suggest that NPWT material interactions with cells, as well as soluble factors including cytokines and LPS, can modulate the immune response, independent of vacuum application. We have also established methodological strategies for studying NPWT materials and reveal the potential utility of cell-based in vitro studies for elucidating biological effects of NPWT materials.

The effect of negative pressure wound therapy with antibacterial dressings or antiseptics on an in vitro wound model

2017 ◽  
Vol 26 (5) ◽  
pp. 236-242 ◽  
Author(s):  
J. Matiasek ◽  
K.J. Domig ◽  
G. Djedovic ◽  
R. Babeluk ◽  
O. Assadian
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Wound Therapy ◽  
Wound Model

scholarly journals The Effect of Negative Pressure Wound Therapy with and without Instillation on Mature Biofilms In Vitro

Materials ◽  
2018 ◽  
Vol 11 (5) ◽  
pp. 811 ◽  
Author(s):  
Shamaila Tahir ◽  
Matthew Malone ◽  
Honghua Hu ◽  
Anand Deva ◽  
Karen Vickery
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Wound Therapy

scholarly journals Technical Support of Wound Healing Processes: Project Status

2019 ◽  
Vol 5 (1) ◽  
pp. 521-523
Author(s):  
Jacquelyn Dawn Parente ◽  
Knut Möller ◽  
Sabine Hensler ◽  
Claudia Kühlbach ◽  
Margareta M. Mueller ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mathematical Models ◽  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Technical Support ◽  
Wound Therapy ◽  
Wound Area ◽  
Modal Treatment ◽  
Healing Processes

AbstractThe optimized wound healing (OWID) project provides technical support of wound healing processes. Advanced biophysical treatment therapies using light (photobiomodulation), negative pressure wound therapy (NPWT), and electrical stimulation show biological effects. Specifically, a biphasic dose-response curve is observed where lower doses activate cells, while above a threshold, higher doses are inhibitory. However, no standard protocols and no multi-modal treatment studies determine specific therapy needs. The OWID project aims to develop a multi-modal treatment device and modelbased therapy for individualized wound healing. This work presents the OWID project status. Currently, a photobiomodulation prototype delivers red, green, and blue light ‘medicine’ at prescribed therapeutic ‘doses’. The calculation of incident light necessarily considers transmission properties of the intervening cell culture plate. Negative pressure wound therapy (NPWT) and electrical impedance tomography (EIT) hardware are being adapted for use in vitro. Development of mathematical models of wound healing and therapy control are supported by treatment experiment outcome measures conducted in a wounded 3D tissue model. Parameter sensitivity analysis conducted on an existing mathematical model of reepithelialization results in changing parameter values influencing cellular movement rates. Thus, the model is robust to fit model parameters to observed reepithelialization rates under treatment conditions impacting cellular activation, inhibition, and untreated controls. Developed image analysis techniques have not captured changes in wound area after photobiomodulation treatment experiments. Alternatively, EIT will be tested for wound area analysis. Additionally, live dyes will be introduced to non-invasively visualize the reepithelialization front on a smaller, cellular scale. Finally, an overall therapeutic feedback control model uses model reference adaptive control to incorporate the intrinsic biological reepithelialization mechanism, treatment loops, and treatment controller modulation at a wound state. Currently, the OWID project conducts photobiomodulation treatment experiments in vitro and has developed mathematical models. Future work includes the incorporation of multi-modal wound healing treatment experiments.

scholarly journals Negative Pressure Wound Therapy Using Polyurethane Foam in a Patient with Necrotizing Fasciitis

2018 ◽  
Vol 55 (4) ◽  
pp. 603-605 ◽  
Author(s):  
Adelaida Avino ◽  
Cristian Radu Jecan ◽  
Cristina Nicoleta Cozma ◽  
Andra Elena Balcangiu Stroescu ◽  
Daniela Gabriela Balan ◽  
...  
Keyword(s):  
Necrotizing Fasciitis ◽  
Polyurethane Foam ◽  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Skin Grafting ◽  
Wound Therapy ◽  
Vein Thrombosis ◽  
History Of ◽  
Deep Vein

Negative pressure wound therapy is one of the newest methods of treatment used in wound healing. An important role of the system has a foam dressing that connects the wound with the vacuum. In general the sponge used in vacuum assisted therapies is made of polyurethane foam. We present the case of a 51-year-old male patient with a history of deep vein thrombosis (known for about 3 years) diagnosed with necrotizing fasciitis (NF). In this patient, after each step of the surgical treatment (large debridement and skin grafting) negative pressure wound therapy using polyurethane foam was used.

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