scholarly journals GAPDH and PUM1: Optimal Housekeeping Genes for Quantitative Polymerase Chain Reaction-Based Analysis of Cancer Stem Cells and Epithelial-Mesenchymal Transition Gene Expression in Rectal Tumors

Cureus ◽  
2020 ◽  
Author(s):  
Litika Vermani ◽  
Rajeev Kumar ◽  
Nachimuthu Senthil Kumar
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Polymerase Chain Reaction ◽  
Epithelial Mesenchymal Transition ◽  
Quantitative Polymerase Chain Reaction ◽  
Housekeeping Genes ◽  
Rectal Tumors ◽  
Chain Reaction ◽  
Mesenchymal Transition ◽  
Polymerase Chain

scholarly journals miR-200c inhibits the invasive activity of primary adamantinomatous craniopharyngioma cells

2019 ◽  
Author(s):  
Hao Liu ◽  
Daobao Zhang ◽  
Zhiyong Liu ◽  
Ruichao Liang ◽  
Tian Meng ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Epithelial Mesenchymal Transition ◽  
Therapy Resistance ◽  
Transwell Assay ◽  
Chain Reaction ◽  
Mesenchymal Transition ◽  
Polymerase Chain ◽  
Adamantinomatous Craniopharyngioma ◽  
E Cadherin

Abstract Backgrounds Craniopharyngiomas are benign epithelial tumors and difficult to complete due to the digitate brain infiltration. miR-200c has been studied in terms of development, stemness, epithelial-mesenchymal transition (EMT), and therapy resistance in many cancers. However, the role of miR-200c remains to be elucidated in adamantinomatous craniopharyngioma.Methods Quantitative real-time polymerase chain reaction was used to evaluate the expression of miR-200c, ZEB1, ZEB2, and CTNNB1. Immunohistochemistry, Western blot, and immunofluorescence analyses were used to evaluate the expression of E-cadherin and β-catenin at the protein level. A Transwell assay was used to evaluate the invasiveness of ten primary craniopharyngioma cell.Results miR-200c was significantly downregulated in adamantinomatous craniopharyngioma compared with papillary craniopharyngioma. Conversely, ZEB1, ZEB2, and CTNNB1 were overexpressed in adamantinomatous craniopharyngioma. Inhibition of miR-200c significantly promoted the invasion of primary adamantinomatous craniopharyngioma cells. Moreover, E-cadherin was overexpressed and β-catenin was downregulated in miR-200c mimic primary adamantinomatous craniopharyngioma cell culture.Conclusion Our data demonstrated that miR-200c maybe reduce the invasive activity of adamantinomatous craniopharyngioma cells through E-cadherin/β-catenin. These findings suggest that the targets of miR-200c may regulate the EMT of adamantinomatous craniopharyngioma.

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