scholarly journals Evaluating gene expression profiling by quantitative polymerase chain reaction to develop a clinically feasible test for outcome prediction in multiple myeloma

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
María E. Sarasquete ◽  
Joaquín Martínez-López ◽  
María C. Chillón ◽  
Miguel Alcoceba ◽  
Luis A. Corchete ◽  
...  
Keyword(s):  
Gene Expression ◽  
Multiple Myeloma ◽  
Polymerase Chain Reaction ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Outcome Prediction ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Myelin-forming cell-specific cadherin-19 is a marker for minimally infiltrative glioblastoma stem-like cells

2015 ◽  
Vol 122 (1) ◽  
pp. 69-77 ◽  
Author(s):  
Michael Zorniak ◽  
Paul A. Clark ◽  
John S. Kuo
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Plasma Membrane ◽  
Glioblastoma Multiforme ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Quantitative Polymerase Chain Reaction ◽  
Normal Brain ◽  
Chain Reaction ◽  
Polymerase Chain

OBJECT Glioblastoma stem-like cells (GSCs) exhibit stem-like properties, are highly efficient at forming tumor xenografts, and are resistant to many current therapies. Current molecular identifiers of GSCs are scarce and controversial. The authors describe differential cell-surface gene expression profiling to identify GSC-specific markers. METHODS Independent human GSC lines were isolated and maintained in standard neural stem cell (NSC) media and were validated for self-renewal, multipotent differentiation, and tumor initiation properties. Candidate upregulated GSCspecific plasma membrane markers were identified through differential Affymetrix U133 Plus 2.0 Array gene expression profiling of GSCs, human NSCs (hNSCs), normal brain tissue, and primary/recurrent glioblastoma multiforme samples. Results were validated by using comparative quantitative reverse transcription polymerase chain reaction and Western blot analysis of GSCs, hNSCs, normal human astrocytes, U87 glioma cell line, and patient-matched serum-cultured glioblastoma multiforme samples. RESULTS A candidate GSC-specific signature of 19 upregulated known and novel plasma membrane–associated genes was identified. Preferential upregulation of these plasma membrane–linked genes was validated by quantitative polymerase chain reaction. Cadherin-19 (CDH19) protein expression was enhanced in minimally infiltrative GSC lines. CONCLUSIONS Gene expression profiling of GSCs has shown CDH19 to be an exciting new target for drug development and study of GBM tumorigenesis.

Real-time polymerase chain reaction-based exponential sample amplification for microarray gene expression profiling

2005 ◽  
Vol 337 (1) ◽  
pp. 76-83 ◽  
Author(s):  
Zsolt B. Nagy ◽  
János Z. Kelemen ◽  
Liliána Z. Fehér ◽  
Ágnes Zvara ◽  
Kata Juhász ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Gene Expression Profiling ◽  
Real Time ◽  
Expression Profiling ◽  
Chain Reaction ◽  
Microarray Gene Expression ◽  
Polymerase Chain ◽  
Microarray Gene

scholarly journals Validation of reference genes for accurate normalization by quantitative polymerase chain reaction in sugarcane drought stress studies using two cultivars

2018 ◽  
Vol 48 (11) ◽  
Author(s):  
Dennis Crystian ◽  
Jackeline Terto ◽  
José Vieira Silva ◽  
Cícero Almeida
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Drought Stress ◽  
Reference Genes ◽  
Quantitative Polymerase Chain Reaction ◽  
Elongation Factor ◽  
Chain Reaction ◽  
Stress Studies ◽  
Accurate Normalization ◽  
Polymerase Chain

ABSTRACT: The aim of this study was to analyze the expression of putative reference genes in sugarcane under drought stress. The varieties RB72454 and RB72910 were cultivated and the treatments control and drought stress applied to 135-day-old plants grown under field conditions. The stress level of the plants was measured by rate of photosynthesis, transpiration, and stomatal conductance. For each biological replicate, expression analyses were conducted using quantitative polymerase chain reaction for the genes α-tubulin, β-tubulin, β-actin, cyclophilin, eukaryotic elongation factor 1, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), histone H3 and ubiquitin. Stability was evaluated using the geNorm, NormFinder, and BestKeeper software packages. Among the candidate genes, GAPDH was identified as the most stable in all software, indicating its suitability for gene expression studies in sugarcane undergoing drought stress; the gene β-actin was the second most stable. These findings suggest using GAPDH and β-actin for normalization in relative gene expression in sugarcane.

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