Saccharification and Single Step Fermentation of Cassava Peel by Mixed Culture of Saccharomycopsis fibuligera NCIM 3161 and Zymomonas mobilis MTCC 92

2016 ◽  
Author(s):  
C. Arun Pandian ◽  
Chandrasekaran Suganya ◽  
Selvaraju Sivamani ◽  
Rajoo Baskar
Keyword(s):  
Mixed Culture ◽  
Zymomonas Mobilis ◽  
Single Step ◽  
Saccharomycopsis Fibuligera ◽  
Cassava Peel

Ethanol production by a mixed culture of flocculent strains of Zymomonas mobilis and Saccharomyces sp.

1996 ◽  
Vol 45 (5) ◽  
pp. 580-583 ◽  
Author(s):  
C. Abate ◽  
D. Callieri ◽  
Emilio Rodríguez ◽  
Oscar Garro
Keyword(s):  
Ethanol Production ◽  
Mixed Culture ◽  
Zymomonas Mobilis

scholarly journals Comparative Study of Ethanol Production from Cassava Pulp by a Mixed Culture of Amylomyces Rouxii with Zygosaccharomyces Pseudorouxii and Zymomonas Mobilis

2019 ◽  
Vol 12 (1) ◽  
pp. 199-210
Author(s):  
Thalisa Yuwa-Amornpitak ◽  
Pa-Nga Yeunyaw
Keyword(s):  
Comparative Study ◽  
Ethanol Production ◽  
Mixed Culture ◽  
Zymomonas Mobilis ◽  
Ethanol Concentration ◽  
Polynomial Equation ◽  
Cassava Pulp ◽  
Optimum Parameters ◽  
Reduced Viscosity ◽  
Order Polynomial

In order to develop a procedure for production bioethanol from cassava pulp, mixed culture of Amylomyces rouxii TISTR 3667 with Zygosaccharomyces pseudorouxii TISTR 5966 or Zymomonas mobilis TISTR 550 and cellulase were evaluated. The parameters such as pH, cellulase, and cassava pulp concentration that influence on the amount of fermentable sugar were optimized by response surface methodology (RSM). Ethanol production was observed and compared to the predicted value that was calculated from the models. The models were fitted to a second-order polynomial equation. They were used to predict ethanol concentration from the use of the mixed culture of A. rouxii TISTR 3667 and Z. mobilis TISTR 550 (G2) that was higher than the amount produced using the mixed culture of A. rouxii TISTR 3667 and Zygosaccharomyces pseudorouxii TISTR 5966 (G1). The following optimum parameters were obtained: pH 6, 20% cassava pulp, and 1% cellulase for G2; and pH 4, 20% cassava pulp, and 0.55% cellulase for G1. The effect of cellulase on ethanol production, a comparative study was conducted in the fermenter by using mixed culture of A. rouxii TISTR 3667 and Z. mobilis TISTR 550. It was showed that more 15% ethanol was gained from 10% cassava pulp with 0.5% cellulase (25 g/l ethanol) compared to the system without cellulase (20 g/l). Mathematically model (equation 4) predicted the ethanol in this system near the actual value of 26.87 g/l. This study indicated that RSM is a powerful tool for optimization fermentation process by using mixed culture including cellulase. Besides these cellulase also reduced viscosity of the cassava medium and enhanced ethanol production. However this process should be more continue to study.

scholarly journals Bioethanol Production from Cassava Peels Inoculated with Saccharomyces cerevisiae and Zymomonas mobilis

2021 ◽  
pp. 58-67
Author(s):  
Kehinde Tope Adegbehingbe ◽  
Foluso Faparusi ◽  
Bartholomew Saanu Adeleke
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Chemical Composition ◽  
Zymomonas Mobilis ◽  
Ph Value ◽  
Control Sample ◽  
Reducing Sugar ◽  
Bioethanol Production ◽  
Transportation Fuel ◽  
Cassava Peels ◽  
Cassava Peel

Aim: This study was designed to determine the bioethanol production from fermented cassava peel using Saccharomyces cerevisiae and Zymomonas mobilis. Methodology: Cassava peels were collected from cassava processing sites, washed, surface sterilized, dried, milled into flour, pretreated, and fermented. Saccharomyces cerevisiae and Z. mobilis suspensions were aseptically inoculated into the fermenting medium and allowed to stand for seven days. The pH, total reducing sugar, chemical composition, and bioethanol composition of the fermenting substrates were determined. Results: A pH decrease from 5.2 to 4.1 was recorded in the sample fermented with S. cerevisiae while the least pH value of 3.8 was obtained from the sample fermented with Z. mobilis, respectively. The total reducing sugar (glucose) of fermented samples decreased from 3.4% to 1.5% (Z. mobilis) and 3.4% to 1.88% (S. cerevisiae) compared with the control sample. The chemical composition showed high protein and fat contents in the fermented samples. High percentage yield of 30% with ethanol volume of 45 mL was recovered from cassava peel inoculated with S. cerevisiae while flash point, i.e. the lowest temperature at which fuel produces enough vapor to cause ignition leading to flame generation of 24oC, was recorded for both fermented samples inoculated with S. cerevisiae and Z. mobilis. Conclusion: The ability of the bacterium and the yeast isolates exhibiting high potential for bioethanol production could be promising in various industrial processes as an alternative to fossil transportation fuel.

Some nutritional requirements of a mixed culture transforming Reichstein's compound S into prednisolone

1992 ◽  
Vol 38 (8) ◽  
pp. 753-757 ◽  
Author(s):  
K. M. Ghanem ◽  
H. H. Yusef
Keyword(s):  
Mixed Culture ◽  
Mycobacterium Smegmatis ◽  
Sodium Acetate ◽  
Single Step ◽  
Curvularia Lunata ◽  
Malt Extract ◽  
Bacterial Inoculation ◽  
Dehydrogenation Reaction ◽  
S 100 ◽  
Exogenous Supply

Reichstein's compound S was successfully converted to prednisolone in a single-step fermentation using a mixed culture of Curvularia lunata and Mycobacterium smegmatis. Introducing additional medium at the time of bacterial inoculation and increasing the M. smegmatis inoculum to 8% were necessary for maximal dehydrogenation of cortisol to prednisolone (86%). However, beef extract, corn-steep solids, and malt extract were inhibitory to the dehydrogenase activity and stimulatory to hydroxylase. Of the vitamins tested, nicotinic acid and riboflavin at 0.2 and 1.13 mg/L, respectively, resulted in maximum transformation of Reichstein's compound S (100%) and optimized prednisolone yields (92%) in the mixed culture. The trace elements present in the medium were sufficient for maximal transformation, and there was no need for an exogenous supply. Addition of ATP, sodium acetate, and NAD inhibited the dehydrogenation reaction. Key words: biotransformation of Reichstein's compound S, mixed-culture transformations, transformation of steroids.

V884: Demonstration of a Novel Single-Step Percutaneous Access Sheath for Nephrostolithotomy: A Video Presentation

2005 ◽  
Vol 173 (4S) ◽  
pp. 240-240
Author(s):  
Premal J. Desai ◽  
David A. Hadley ◽  
Lincoln J. Maynes ◽  
D. Duane Baldwin
Keyword(s):  
Single Step ◽  
Video Presentation ◽  
Percutaneous Access ◽  
Access Sheath
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