scholarly journals Characterization and localization of cyclin B3 transcript in both oocyte and spermatocyte of the rainbow trout (Oncorhynchus mykiss)

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7396
Author(s):  
Wenzhi Guan ◽  
Liangjie Qiu ◽  
Bo Zhang ◽  
Jianbo Yao ◽  
Qing Xiao ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Expression Profiles ◽  
Cyclin B1 ◽  
Amino Acid Residues ◽  
Regulatory Subunits ◽  
Reading Frame ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Mitotic Cyclin ◽  
Cyclin B3

B-type cyclins are regulatory subunits with distinct roles in the cell cycle. To date, at least three subtypes of B-type cyclins (B1, B2, and B3) have been identified in vertebrates. Previously, we reported the characterization and expression profiles of cyclin B1 and B2 during gametogenesis in the rainbow trout (Oncorhynchus mykiss). In this paper, we isolated another subtype of cyclin B, cyclin B3 (CB3), from a cDNA library of the rainbow trout oocyte. The full-length CB3 cDNA (2,093 bp) has an open reading frame (1,248 bp) that encodes a protein of 416 amino acid residues. The CB3 transcript was widely distributed in all the examined tissues, namely, eye, gill, spleen, brain, heart, kidney, stomach, skin, muscle, and, especially, gonad. Northern blot analysis indicated only one form of the CB3 transcript in the testis and ovary. In situ hybridization revealed that, in contrast to cyclin B1 and B2 transcripts, CB3 transcripts were localized in the oocytes, spermatocytes, and spermatogonia. These findings strongly suggest that CB3 plays a role not only as a mitotic cyclin in spermatogonial proliferation during early spermatogenesis but also during meiotic maturation of the spermatocyte and oocyte in the rainbow trout.

Cloning and sequence analysis of the cDNA encoding P-450 aromatase (P450arom) from a rainbow trout (Oncorhynchus mykiss) ovary; relationship between the amount of P450arom mRNA and the production of oestradiol-17β in the ovary

1992 ◽  
Vol 8 (1) ◽  
pp. 53-61 ◽  
Author(s):  
M. Tanaka ◽  
T. M. Telecky ◽  
S. Fukada ◽  
S. Adachi ◽  
S. Chen ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Northern Blot Analysis ◽  
Ovarian Development ◽  
Open Reading Frame ◽  
Amino Acid Residues ◽  
Cdna Insert ◽  
Reading Frame ◽  
Rna Transcripts ◽  
Rainbow Trout Oncorhynchus Mykiss

ABSTRACT The enzyme aromatase P-450 (P450arom) catalyses the conversion of androgen to oestrogen. A cDNA insert encoding P450arom was isolated from a rainbow trout (Oncorhynchus mykiss) ovary cDNA library. The insert was sequenced and found to contain an open-reading frame predicted to encode a protein of 522 amino acid residues. The deduced polypeptide is 52% homologous with human, mouse and rat P450arom and 53% homologous with that of chicken. The insert was confirmed to encode P450arom by introducing it into COS-1 monkey kidney tumour cells (COS-1 cells) and detecting the conversion of testosterone to oestradiol-17β by radioimmunoassay. The N-terminal region of the deduced polypeptide was 19 amino acids longer than that of the other four species, and was found by hydropathy plotting to be very hydrophobic. Northern blot analysis revealed 2.6kb RNA transcripts which were present in the trout ovary during vitellogenesis and hybridized to the cDNA insert. In preparations from subsequent stages of ovarian development, no RNA transcripts hybridized to the probe. Since the RNA transcripts are present only during the stage of oestradiol-β production by the ovarian follicles, oestradiol-17β production may be regulated, in part, by the amount of P450arom mRNA present.

scholarly journals Hepatic phosphoenolpyruvate carboxykinase gene expression is not repressed by dietary carbohydrates in rainbow trout (Oncorhynchus mykiss)

2001 ◽  
Vol 204 (2) ◽  
pp. 359-365 ◽  
Author(s):  
S. Panserat ◽  
E. Plagnes-Juan ◽  
J. Breque ◽  
S. Kaushik
Keyword(s):  
Gene Expression ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Nutritional Regulation ◽  
Dietary Carbohydrates ◽  
Base Pairs ◽  
Reading Frame ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Highly Active

Phosphoenolpyruvate carboxykinase (PEPCK) is a rate-limiting enzyme in hepatic gluconeogenesis and therefore plays a central role in glucose homeostasis. The aim of this study was to analyse the nutritional regulation of PEPCK gene expression in rainbow trout (Oncorhynchus mykiss), which are known to use dietary carbohydrates poorly. A full-length hepatic PEPCK cDNA (2637 base pairs with one open reading frame putatively encoding a 635-residue protein) was cloned and found to be highly homologous to mammalian PEPCKs. The presence of a putative peptide signal specific to a mitochondrial-type PEPCK in the deduced amino acid sequence suggests that this PEPCK gene codes for a mitochondrial form. In gluconeogenic tissues such as liver, kidney and intestine, this PEPCK gene was expressed at high levels and, in the liver we found no regulation of PEPCK gene expression by dietary carbohydrates. These results suggest that the first step of the hepatic gluconeogenic pathway in rainbow trout is functional and highly active irrespective of the dietary carbohydrate supply.

scholarly journals Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Artur Gurgul ◽  
Klaudia Pawlina-Tyszko ◽  
Monika Bugno-Poniewierska ◽  
Tomasz Szmatoła ◽  
Igor Jasielczuk ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Hydrostatic Pressure ◽  
Oncorhynchus Mykiss ◽  
High Hydrostatic Pressure ◽  
Expression Profiles ◽  
Polar Body ◽  
Rna Integrity ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
The Difference ◽  
Second Polar Body

High hydrostatic pressure (HHP) causes depolymerization of the spindle microtubules. HHP applied to fish eggs prevents extrusion of the second polar body and inhibits the first cell cleavage, and it is used to produce triploids and diploid gynogenetic and androgenetic individuals. HHP has been also found to affect biomolecules including nucleic acids, and it may be presumed that HHP administered to the rainbow trout (Oncorhynchus mykiss) eggs disturbs cytoplasmic maternal RNA indispensable for the early embryogenesis. To verify this assumption, quality and quantity of RNA extracted from the rainbow trout eggs subjected to the high hydrostatic pressure shock were analyzed. Provided results exhibited that maternal transcriptome was resistant to a three-minute exposure to 65.5 MPa of HHP treatment. Some trend showing increase of the RNA integrity was observed in the HHP-treated eggs; however, the difference was not statistically significant. Alterations in the expression profiles in the rainbow trout eggs subjected to HHP were also negligible. Greater differences in the maternal gene expression were observed between eggs from different clutches than between HHP-treated and untreated eggs from the same clutch. It may be assumed that exposure to HHP shock was too short to modify significantly maternal transcripts in the rainbow trout eggs.

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