scholarly journals The structure and functional profile of ruminal microbiota in young and adult reindeers (Rangifer tarandus) consuming natural winter-spring and summer-autumn seasonal diets

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12389
Author(s):  
Elena Yildirim ◽  
Larisa Ilina ◽  
Georgy Laptev ◽  
Valentina Filippova ◽  
Evgeni Brazhnik ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
Seasonal Changes ◽  
Volatile Fatty Acids ◽  
Rangifer Tarandus ◽  
Winter Season ◽  
Gene Families ◽  
Climatic Conditions ◽  
Acid Fermentation ◽  
Age Related ◽  
Ruminal Microbiota

Background The key natural area of Russian reindeer (Rangifer tarandus, Nenets breed) is arctic zones, with severe climatic conditions and scarce feed resources, especially in the cold winter season. The adaptation of reindeer to these conditions is associated not only with the genetic potential of the animal itself. The rumen microbiome provides significant assistance in adapting animals to difficult conditions by participating in the fiber digestion. The aim of our study is to investigate the taxonomy and predicted metabolic pathways of the ruminal microbiota (RM) during the winter–spring (WS) and summer–autumn (SA) seasons, in calves and adult reindeer inhabiting the natural pastures of the Yamalo-Nenetsky Autonomous District of the Russian Federation. Methods The RM in reindeer was studied using the Next Generation Sequencing method with the MiSeq (Illumina, San Diego, CA, USA) platform. Reconstruction and prediction of functional profiles of the metagenome, gene families, and enzymes were performed using the software package PICRUSt2 (v.2.3.0). Results The nutritional value of WS and SA diets significantly differed. Crude fiber content in the WS diet was higher by 22.4% (p < 0.05), compared to SA, indicating possibly poorer digestibility and necessity of the adaptation of the RM to this seasonal change. A total of 22 bacterial superphyla and phyla were found in the rumen, superphylum Bacteroidota and phylum Firmicutes being the dominating taxa (up to 48.1% ± 4.30% and 46.1% ± 4.80%, respectively); while only two archaeal phyla presented as minor communities (no more then 0.54% ± 0.14% totally). The percentages of the dominating taxa were not affected by age or season. However, significant changes in certain minor communities were found, with seasonal changes being more significant than age-related ones. The percentage of phylum Actinobacteriota significantly increased (19.3-fold) in SA, compared to WS (p = 0.02) in adults, and the percentage of phylum Cyanobacteria increased up to seven-fold (p = 0.002) in adults and calves. Seasonal changes in RM can improve the ability of reindeer to withstand the seasons characterized by a low availability of nutrients. The PICRUSt2 results revealed 257 predicted metabolic pathways in RM: 41 pathways were significantly (p < 0.05) influenced by season and/or age, including the processes of synthesis of vitamins, volatile fatty acids, and pigments; metabolism of protein, lipids, and energy; pathogenesis, methanogenesis, butanediol to pyruvate biosynthesis, cell wall biosynthesis, degradation of neurotransmitters, lactic acid fermentation, and biosynthesis of nucleic acids. A large part of these changeable pathways (13 of 41) was related to the synthesis of vitamin K homologues. Conclusion The results obtained improve our knowledge on the structure and possible metabolic pathways of the RM in reindeer, in relation to seasonal changes.

scholarly journals The Comparative Analysis of the Ruminal Bacterial Population in Reindeer (Rangifer tarandus L.) from the Russian Arctic Zone: Regional and Seasonal Effects

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 911
Author(s):  
Larisa A. Ilina ◽  
Valentina A. Filippova ◽  
Evgeni A. Brazhnik ◽  
Andrey V. Dubrovin ◽  
Elena A. Yildirim ◽  
...  
Keyword(s):  
Rangifer Tarandus ◽  
Microbial Population ◽  
Cellulose Degradation ◽  
Winter Season ◽  
Influential Factors ◽  
Seasonal Effects ◽  
Rrna Gene ◽  
Ambient Temperatures ◽  
Autonomous District ◽  
Ruminal Microbiota

The reindeer (Rangifer tarandus L.) is a unique animal inhabitant of arctic regions. Low ambient temperatures and scant diets (primarily, lichens) have resulted in different evolutional adaptations, including the composition of the ruminal microbiota. In the study presented here, the effects of seasonal and regional aspects of the composition of the ruminal microbiota in reindeer (Nenets breed, 38 animals) were studied (wooded tundra from the Yamalo-Nenetski Autonomous District (YNAD) vs. from the Nenetski Autonomous District (NAD)). The ruminal content of calves (n = 12) and adult animals (n = 26, 15 males and 11 females) was sampled in the summer (n = 16) and winter seasons (n = 22). The composition of the ruminal microbial population was determined by the V3-V4 16S rRNA gene region sequencing. It was found that the population was dominated by Bacteroidetes and Firmicutes phyla, followed by Spirochaetes and Verrucomicrobia. An analysis of the community using non-metric multidimensional scaling and Bray–Curtis similarity metrics provided evidence that the most influential factors affecting the composition of ruminal microbiota are the region (p = 0.001) and season (p = 0.001); heat map analysis revealed several communities that are strongly affected by these two factors. In the summer season, the following communities were significantly larger compared to in the winter season: Coriobactriaceae, Erysipelothrihaceae, and Mycoplasmataceae. The following communities were significantly larger in the winter season compared to in summer: Paraprevotellaceae, Butyrivibrio spp., Succiniclasticum spp., Coprococcus spp., Ruminococcus spp., and Pseudobutyrivibrio spp. In NAD (tundra), the following communities were significantly larger in comparison to YNAD (wooded tundra): Verrucomicrobia (Verruco-5), Anaerolinaceae, PeHg47 Planctomycetes, cellulolytic Lachnospiraceae, and Succiniclasticum spp. The following bacterial groups were significantly larger in YNAD in comparison to NAD: cellulolytic Ruminococaceae, Dehalobacteriaceae, Veillionelaceae, and Oscilospira spp. The significant differences in the ruminal microbial population were primarily related to the ingredients of diets, affected by region and season. The summer-related increases in the communities of certain pathogens (Mycoplasmataceae, Fusobacterium spp., Porphyromonas endodentalis) were found. Regional differences were primarily related to the ratio of the species involved in ruminal cellulose degradation and ruminal fatty acids metabolism; these differences reflect the regional dissimilarities in botanical diet ingredients.

Effect of seasonal climatic variance on water main failure frequencies in moderate climate regions

2013 ◽  
Vol 13 (2) ◽  
pp. 435-446 ◽  
Author(s):  
D. Fuchs-Hanusch ◽  
F. Friedl ◽  
R. Scheucher ◽  
B. Kogseder ◽  
D. Muschalla
Keyword(s):  
Water Distribution ◽  
Performance Indicator ◽  
Winter Season ◽  
Climatic Conditions ◽  
Third Party ◽  
Structural Quality ◽  
Failure Frequency ◽  
Age Related ◽  
Water Main ◽  
Moderate Climate

The yearly water main failure frequency is a central performance indicator to describe the structural quality of a water distribution network. Besides age related deterioration, events such as severe climatic conditions or intensified third party construction may cause sudden seasonal increases in failure frequency trends. For the cold regions of Canada and the dry regions of Australia, several studies exist describing the impacts of climatic failure frequencies on water main failure variations. Failure prediction modelling applied to Austrian supply systems have shown that irregularities in overall failure trends were not explainable with commonly used model covariates like material, vintage, diameter or the number of previous breaks. Analysing the monthly failure frequencies of several Austrian utilities, seasonal differences and variations in failure frequencies are recognizable. The research described in this paper therefore focused on analysing if climatic impacts are responsible for these variations. In a first step, climatic indicators, which are able to describe seasonal climatic variations in moderate climate regions, were derived. In a next step the correlation between summer and winter failure frequencies to these climatic indicators was analysed. The indicators taken into account were, e.g. the decisive freezing index (DFI), the summer rain deficit (RDs) or the amount of successive hot days (AHD). The research has shown that in all investigated climatic zones of Austria, the severity of the winter season influences failure frequencies. A dependency between winter failure frequency and the DFI was significant especially for rigid material types and for diameters up to 200 mm. So far, soil moisture effects have only shown a slight significance. Nevertheless, the indicator AHD has shown a correlation to failure frequencies in the dryer climate zones of Austria. This is of further interest as it is very likely that the AHD is going to increase due to climate change.

Adaptive changes of the hair coat of the horses of zabaykalsky aborigenous breed

2019 ◽  
Vol 49 (4) ◽  
pp. 101-105 ◽  
Author(s):  
G. M. Shkyratova ◽  
B. Z. Bazaron ◽  
T. N. Khamiruev ◽  
S. M. Dashinimaev
Keyword(s):  
Seasonal Changes ◽  
Climatic Conditions ◽  
Skin Thickness ◽  
Summer Period ◽  
Hair Coat ◽  
Skin Fold ◽  
Winter Grazing ◽  
Shoulder Blade ◽  
Quantitative Indicators

The seasonal changes in the skin thickness and structure of the horses’ coat, as signs of adaptation to environmental factors, were studied. The experiment was carried out with the livestock kept in a herd using winter-grazing technology without additional feedings in the climatic conditions of the Trans-Baikal Territory. The objects of the research were adult mares of Zabaikalsky breed of horses of the same age, class and fatness. The studies were carried out in the middle of each season (May, July, October, February). The length of the coat was measured with a caliper, the coat itself with the determination of the ratio of hair (fl uffy hair, heterotype hair and coarse hair) and the thickness of the skin fold were measured in accordance with the approved methodological recommendations. The minimum skin thickness in winter was detected in mares on the back and shoulder blade – 4.3 and 4.4 mm, the maximum – on the side and thigh – 4.5 4.6 mm. When compared with the summer period, the increase on the side was 0.8 mm, whereas on the back, shoulder blade and thigh – 0.4 mm (p ≤ 0,001). In spring, thickening of the skin was noted within 0.1-0.3 mm in the same topographic areas, compared to autumn. The quantitative indicators of the coat changed depending on the season of the year. In winter, the coat contained more fl uffy hair (23.10%), and less coarse hair (68.24%), in summer there was a lower content of fl uffy hair (4.33%), but more coarse hair (94.01%.) Sharp seasonal changes were noted with regard to the length of the hair. The longest hair was found in winter and spring – 4.96 and 4.26 cm, whereas the shortest – in summer and autumn – 0.94 and 1.90 cm, respectively.

scholarly journals Long-term Diet Quality and Gut Microbiome Functionality: A Prospective, Shotgun Metagenomic Study among Urban Chinese Adults

2021 ◽  
Vol 5 (4) ◽  
Author(s):  
Danxia Yu ◽  
Yaohua Yang ◽  
Jirong Long ◽  
Wanghong Xu ◽  
Qiuyin Cai ◽  
...  
Keyword(s):  
Diet Quality ◽  
Gut Microbiome ◽  
Metabolic Pathways ◽  
Gene Families ◽  
Diet Group ◽  
Healthy Diet ◽  
Chinese Adults ◽  
Α Diversity ◽  
Unhealthy Diet

ABSTRACT Background Diet is known to affect human gut microbiome composition; yet, how diet affects gut microbiome functionality remains unclear. Objective We compared the diversity and abundance/presence of fecal microbiome metabolic pathways among individuals according to their long-term diet quality. Methods In 2 longitudinal cohorts, we assessed participants’ usual diets via repeated surveys during 1996–2011 and collected a stool sample in 2015–2018. Participants who maintained a healthy or unhealthy diet (i.e., stayed in the highest or lowest quintile of a healthy diet score throughout follow-up) were selected. Participants were excluded if they reported a history of cancer, cardiovascular disease, diabetes, or hypertension; had diarrhea or constipation in the last 7 d; or used antibiotics in the last 6 mo before stool collection. Functional profiling of shotgun metagenomics was performed using HUMAnN2. Associations of dietary variables and 420 microbial metabolic pathways were evaluated via multivariable-adjusted linear or logistic regression models. Results We included 144 adults (mean age = 64 y; 55% female); 66 had an unhealthy diet and 78 maintained a healthy diet. The healthy diet group had higher Shannon α-diversity indexes of microbial gene families and metabolic pathways (both P &lt; 0.02), whereas β-diversity, as evaluated by Bray-Curtis distance, did not differ between groups (both P &gt; 0.50). At P &lt; 0.01 [false discovery rate (FDR) &lt;0.15], the healthy diet group showed enriched pathways for vitamin and carrier biosynthesis (e.g., tetrahydrofolate, acetyl-CoA, and l-methionine) and tricarboxylic acid (TCA) cycle, and increased degradation (or reduced biosynthesis) of certain sugars [e.g., cytidine monophosphate (CMP)-legionaminate, deoxythymidine diphosphate (dTDP)-l-rhamnose, and sucrose], nucleotides, 4-aminobutanoate, methylglyoxal, sulfate, and aromatic compounds (e.g., catechol and toluene). Meanwhile, several food groups were associated with the CMP-legionaminate biosynthesis pathway at FDR &lt;0.05. Conclusions In a small longitudinal study of generally healthy, older Chinese adults, we found long-term healthy eating was associated with increased α-diversity of microbial gene families and metabolic pathways and altered symbiotic functions relevant to human nutrition and health.

scholarly journals Changes in Air Temperature and Snow Cover in Winter in Poland

Atmosphere ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 68
Author(s):  
Arkadiusz M. Tomczyk ◽  
Ewa Bednorz ◽  
Katarzyna Szyga-Pluta
Keyword(s):  
Snow Cover ◽  
Air Temperature ◽  
Winter Season ◽  
Primary Objective ◽  
Climatic Conditions ◽  
Cover Depth ◽  
North Eastern ◽  
Northern Regions ◽  
Maximum Air Temperature ◽  
Snow Cover Depth

The primary objective of the paper was to characterize the climatic conditions in the winter season in Poland in the years 1966/67–2019/20. The study was based on daily values of minimum (Tmin) and maximum air temperature (Tmax), and daily values of snow cover depth. The study showed an increase in both Tmin and Tmax in winter. The most intensive changes were recorded in north-eastern and northern regions. The coldest winters were recorded in the first half of the analyzed multiannual period, exceptionally cold being winters 1969/70 and 1984/85. The warmest winters occurred in the second half of the analyzed period and among seasons with the highest mean Tmax, particularly winters 2019/20 and 1989/90 stood out. In the study period, a decrease in snow cover depth statistically significant in the majority of stations in Poland was determined, as well as its variability both within the winter season and multiannual.

scholarly journals Formaldehyde and De/Methylation in Age-Related Cognitive Impairment

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 913
Author(s):  
Ting Li ◽  
Yan Wei ◽  
Meihua Qu ◽  
Lixian Mou ◽  
Junye Miao ◽  
...  
Keyword(s):  
Amino Acids ◽  
Cell Proliferation ◽  
Cognitive Impairment ◽  
Metabolic Pathways ◽  
Memory Formation ◽  
Epigenetic Alterations ◽  
Diagnostic Biomarker ◽  
Reactive Substance ◽  
Age Related ◽  
Novel Therapeutic

Formaldehyde (FA) is a highly reactive substance that is ubiquitous in the environment and is usually considered as a pollutant. In the human body, FA is a product of various metabolic pathways and participates in one-carbon cycle, which provides carbon for the synthesis and modification of bio-compounds, such as DNA, RNA, and amino acids. Endogenous FA plays a role in epigenetic regulation, especially in the methylation and demethylation of DNA, histones, and RNA. Recently, epigenetic alterations associated with FA dysmetabolism have been considered as one of the important features in age-related cognitive impairment (ARCI), suggesting the potential of using FA as a diagnostic biomarker of ARCI. Notably, FA plays multifaceted roles, and, at certain concentrations, it promotes cell proliferation, enhances memory formation, and elongates life span, effects that could also be involved in the aetiology of ARCI. Further investigation of and the regulation of the epigenetics landscape may provide new insights about the aetiology of ARCI and provide novel therapeutic targets.

scholarly journals Seasonal Changes in Lung Function in a Farming Population

2000 ◽  
Vol 7 (4) ◽  
pp. 320-325 ◽  
Author(s):  
Ambikaipakan Senthilselvan ◽  
James A Dosman ◽  
Karen M Semchuk ◽  
Helen H McDuffie ◽  
Allan J Cessna ◽  
...  
Keyword(s):  
Pulmonary Function ◽  
Seasonal Changes ◽  
Pesticide Exposure ◽  
Respiratory Health ◽  
Longitudinal Design ◽  
Winter Season ◽  
Occupational Exposures ◽  
Population Based ◽  
Forced Expiratory Volume ◽  
Farm Residents

OBJECTIVE:To assess the changes in respiratory health from winter to summer seasons in a rural population.DESIGN:A longitudinal design was used in the study.SETTING:A population-based study was conducted as part of the Environmental Pesticide Exposure and Human Health component of the Prairie Ecosystem Study (PECOS) in southwestern Saskatchewan.PATIENTS:In the winter season, 358 patients participated in the study. Of these patients, 234 returned for the second assessment during the summer season. After excluding 34 children aged 17 years and under, 200 adult patients were available for analysis.MEASUREMENTS:Questionnaires were used to obtain information on demographic factors, smoking habits, occupational and environmental exposures, and respiratory conditions. Pulmonary function measurements were obtained using a volume displacement spirometer.RESULTS:Mean ages (± SD) of the 106 men and 94 women participating in the study were 50.1±13.3 and 49.0±13.1 years, respectively. Mean percentage changes in maximal midexpiratory flow rate from winter to summer assessments indicated an improvement for town residents and a decline for farm residents. Mean percentage changes in the ratio of forced expiratory volume in 1 s to forced vital capacity indicated an improvement for town residents who were not engaged in farming, and increasing declines for town residents engaged in farming, farm residents not engaged in farming and farm residents engaged in farming.CONCLUSIONS:Seasonal changes occurred in measurements of pulmonary function between winter and summer seasons; these changes may be related to the environmental or occupational exposures experienced by the participants during the study.

Field survey on frost damage to roof tiles under climatic conditions

2016 ◽  
Vol 34 (2) ◽  
pp. 135-149 ◽  
Author(s):  
Chiemi Iba ◽  
Ayumi Ueda ◽  
Shuichi Hokoi
Keyword(s):  
Field Survey ◽  
Winter Season ◽  
Frost Damage ◽  
Climatic Conditions ◽  
Weather Data ◽  
Measured Temperature ◽  
Content Type ◽  
Roof Tile ◽  
Deterioration Process ◽  
The Impact

Purpose – Frost damage is well-known as the main cause of roof tile deterioration. The purpose of this paper is to develop an analytical model for predicting the deterioration process under certain climatic conditions. This paper describes the results of a field survey conducted to acquire fundamental information useful to this aim. Design/methodology/approach – A field survey of roof tile damage by freezing was conducted in an old temple precinct in Kyoto, Japan. Using detailed observations and photographic recordings, the damage progress was clarified. To examine the impact of climatic conditions upon the damage characteristics, weather data and roof tile temperatures were measured and logged in the winter season. Findings – The deterioration process was observed under the climatic conditions associated with the measured temperature of the roof tiles. In particular, it was revealed that the orientation has a significant influence on increasing or decreasing the risk of frost damage. For certain distinctive forms of damage, the deterioration mechanisms were estimated from the viewpoint of the moisture flow and temperature distribution in the tile. Originality/value – This study contributes to the elucidation of the mechanism behind frost damage to roof tiles. The findings will guide the construction of a numerical model for frost damage prediction.

Anti-aging natural compounds and their role in the regulation of metabolic pathways leading to longevity

2021 ◽  
Vol 21 ◽  
Author(s):  
Aqsa Muzammil ◽  
Muhammad Waqas ◽  
Ahitsham Umar ◽  
Muhammad Sufyan ◽  
Abdur Rehman ◽  
...  
Keyword(s):  
Natural Products ◽  
Metabolic Pathways ◽  
Natural Compounds ◽  
Chronic Obstructive ◽  
Telomere Shortening ◽  
Age Related ◽  
Chronic Obstructive Pulmonary Diseases ◽  
Extended Lifespan ◽  
Ancient Times

: Aging is an unavoidable process, leads to cell senescence due to physiochemical changes in an organism. Anti-aging remedies have always been of great interest since ancient times. The purpose of anti-aging activities is to increase the life span and the quality of life. Anti-aging activities are primarily involved in the therapies of age-related disorders such as Parkinson's disease (PD), Alzheimer's disease (AD), cardiovascular diseases, cancer and chronic obstructive pulmonary diseases. These diseases are triggered by multiple factors that are involved in numerous molecular pathways including telomere shortening, NF-κB pathway, adiponectin receptor pathway, insulin and IGF signaling pathway, AMPK, mTOR and mitochondria dysfunction. Natural products are known as effective molecules to delay the aging process through influencing metabolic pathways and thus ensure an extended lifespan. These natural compounds are being utilized in drug design and development through computational and high throughput techniques for effective pro-longevity drugs. A comprehensive study of natural compounds demonstrated with their anti-aging activities along with databases of natural products for drug designing was executed and summarized in this review article.

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