scholarly journals No adverse effect of a maternal high carbohydrate diet on their offspring, in rainbow trout (Oncorhynchus mykiss)

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12102
Author(s):  
Therese Callet ◽  
Hongyan Li ◽  
Anne Surget ◽  
Frederic Terrier ◽  
Franck Sandres ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
High Carbohydrate ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Low Protein ◽  
Promising Solution ◽  
Males And Females ◽  
Broodstock Diet ◽  
Protein Rich ◽  
First Time

In order to develop a sustainable salmonid aquaculture, it is essential to continue to reduce the use of the protein-rich fishmeal. One promising solution to do so is the use of plant-derived carbohydrates in diet destined to broodstock. However, in mammals, the reduction of protein content (replaced by carbohydrates) in parental diet is known to have strong adverse effects on offspring phenotypes and metabolism. For the first time, the effect of a paternal and a maternal high carbohydrate-low protein diet was assessed on progeny at long term in the rainbow trout. A 30% protein diminution in both males and females broodstock diet during 10 month and 5 months, respectively, did not trigger adverse consequences on their offspring. At the molecular level, offspring transcriptomes were not significantly altered, emphasizing no effect on metabolism. Tenuous differences in the biochemical composition of the liver and the viscera were observed. The recorded effects remained in the normal range of value and accordingly offspring growth were not negatively affected over the long term. Overall, we demonstrated here that a 30% protein diminution during gametogenesis is feasible, confirming the possibility to increase the proportion of plant-derived carbohydrates in female broodstock diets to replace fishmeal proteins.

scholarly journals Apparent low ability of liver and muscle to adapt to variation of dietary carbohydrate:protein ratio in rainbow trout (Oncorhynchus mykiss)

2012 ◽  
Vol 109 (8) ◽  
pp. 1359-1372 ◽  
Author(s):  
Sandrine Skiba-Cassy ◽  
Stéphane Panserat ◽  
Mélanie Larquier ◽  
Karine Dias ◽  
Anne Surget ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Amino Acid ◽  
Oncorhynchus Mykiss ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Futile Cycle ◽  
High Carbohydrate ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Low Protein ◽  
Expression Of Genes

The rainbow trout (Oncorhynchus mykiss) exhibits high dietary amino acid requirements and an apparent inefficiency to use dietary carbohydrates. Using this species, we investigated the metabolic consequences of long-term high carbohydrates/low protein feeding. Fish were fed two experimental diets containing either 20 % carbohydrates/50 % proteins (C20P50), or high levels of carbohydrates at the expense of proteins (35 % carbohydrates/35 % proteins – C35P35). The expression of genes related to hepatic and muscle glycolysis (glucokinase (GK), pyruvate kinase and hexokinase) illustrates the poor utilisation of carbohydrates irrespective of their dietary levels. The increased postprandial GK activity and the absence of inhibition of the gluconeogenic enzyme glucose-6-phosphatase activity support the hypothesis of the existence of a futile cycle around glucose phosphorylation extending postprandial hyperglycaemia. After 9 weeks of feeding, the C35P35-fed trout displayed lower body weight and feed efficiency and reduced protein and fat gains than those fed C20P50. The reduced activation of eukaryotic translation initiation factor 4-E binding protein 1 (4E-BP1) in the muscle in this C35P35 group suggests a reduction in protein synthesis, possibly contributing to the reduction in N gain. An increase in the dietary carbohydrate:protein ratio decreased the expression of genes involved in amino acid catabolism (serine dehydratase and branched-chain α-keto acid dehydrogenase E1α and E1β), and increased that of carnitine palmitoyltransferase 1, suggesting a higher reliance on lipids as energy source in fish fed high-carbohydrate and low-protein diets. This probably also contributes to the lower fat gain. Together, these results show that different metabolic pathways are affected by a high-carbohydrate/low-protein diet in rainbow trout.

scholarly journals Short-Term Effect of a Low-Protein High-Carbohydrate Diet on Mature Female and Male, and Neomale Rainbow Trout

2021 ◽  
Vol 22 (11) ◽  
pp. 6149
Author(s):  
Nathan Favalier ◽  
Vincent Véron ◽  
Michael Marchand ◽  
Anne Surget ◽  
Patrick Maunas ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Lipid Metabolism ◽  
Mature Female ◽  
High Carbohydrate Diet ◽  
Short Term ◽  
Carbohydrate Diet ◽  
High Carbohydrate ◽  
Glucose And Lipid Metabolism ◽  
Low Protein ◽  
First Time

Rainbow trout are considered as a poor user of dietary carbohydrates, displaying persistent postprandial hyperglycaemia when fed a diet containing high amounts of carbohydrates. While this phenotype is well-described in juveniles, less attention was given to broodstock. Our objective was to assess for the first time the short-term consequences of feeding mature female and male, and neomale trout with a low-protein high-carbohydrate diet on glucose and lipid metabolism. Fish were fed for two days with a diet containing either no or 32% of carbohydrates. We analysed plasma metabolites, mRNA levels and enzymatic activities of glycolysis, gluconeogenesis, de novo lipogenesis and β-oxidation in the liver. Results demonstrated that the glucose and lipid metabolism were regulated by the nutritional status in all sexes, irrespective of the carbohydrate intake. These data point out that carbohydrate intake during a short period (5 meals) at 8 °C did not induce specific metabolic changes in broodstock. Finally, we demonstrated, for the first time, sex differences regarding the consequences of two days of feeding on glucose and lipid metabolism.

scholarly journals Modulation of Energy Metabolism and Epigenetic Landscape in Rainbow Trout Fry by a Parental Low Protein/High Carbohydrate Diet

Biology ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 585
Author(s):  
Thérèse Callet ◽  
Hongyan Li ◽  
Pascale Coste ◽  
Stéphane Glise ◽  
Cécile Heraud ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Energy Metabolism ◽  
Mitochondrial Dynamics ◽  
Control Diet ◽  
Epigenetic Landscape ◽  
Carbohydrate Diet ◽  
High Carbohydrate ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
First Feeding ◽  
Low Protein

It is now recognized that parental diets could highly affect offspring metabolism and growth. Studies in fish are, however, lacking. In particular, the effect of a parental diet high in carbohydrate (HC) and low in protein (LP) on progeny has never been examined in higher trophic level teleost fish. Thus, two-year old male and female rainbow trout (Oncorhynchus mykiss) were fed either a control diet (0% carbohydrate and 63.89% protein) or a diet containing 35% carbohydrate and 42.96% protein (HC/LP) for a complete reproductive cycle for females and over a 5-month period for males. Cross-fertilizations were then carried out. To evaluate the effect of the parental diet on their offspring, different phenotypic and metabolic traits were recorded for offspring before their first feeding and again three weeks later. When considering the paternal and maternal HC/LP nutrition independently, fry phenotypes and transcriptomes were only slightly affected. The combination of the maternal and paternal HC/LP diets altered the energy metabolism and mitochondrial dynamics of their progeny, demonstrating the existence of a synergistic effect. The global DNA methylation of whole fry was also highly affected by the HC/LP parental diet, indicating that it could be one of the fundamental mechanisms responsible for the effects of nutritional programming.

scholarly journals Transgenic rainbow trout expressed sGnRH-antisense RNA under the control of sGnRH promoter of Atlantic salmon

2000 ◽  
Vol 25 (3) ◽  
pp. 337-350 ◽  
Author(s):  
S Uzbekova ◽  
J Chyb ◽  
F Ferriere ◽  
T Bailhache ◽  
P Prunet ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Atlantic Salmon ◽  
Transgenic Fish ◽  
Transgenic Animals ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Males And Females ◽  
Mature Fish ◽  
First Time ◽  
The Brain ◽  
F2 Progeny

A recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from a corresponding sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs. This resulted in transgenic animals that had integrated one copy of the transgene into their genome and transmitted it through the germline. Antisense-sGnRH mRNA (AS) was expressed mainly in the brain of transgenic AS(+) fish. Levels of sGnRH endogenous mRNA in the brain were lower in 11-month-old AS(+) fish compared with nontransgenic AS(-) individuals from the same F2 progeny. sGnRH levels significantly decreased in the pituitary of transgenic males and females around the maturation period and in the brain of AS(+) immature females compared with controls. No reliable statistical difference was found in the levels of FSH and LH between AS(+) and AS(-) groups either in immature or mature fish. The majority of transgenic fish reached maturity at the same time as did nontransgenic individuals, although the maturation of AS(+) animals seemed to be more asynchronous. For the first time, the influence of antisense messengers on endogenous mRNA in transgenic fish and the corresponding protein is described.

scholarly journals Long-term exposure to low 17α-ethinylestradiol (EE2) concentrations disrupts both the reproductive and the immune system of juvenile rainbow trout, Oncorhynchus mykiss

2020 ◽  
Vol 142 ◽  
pp. 105836
Author(s):  
Kristina Rehberger ◽  
Elena Wernicke von Siebenthal ◽  
Christyn Bailey ◽  
Patrick Bregy ◽  
Melanie Fasel ◽  
...  
Keyword(s):  
Immune System ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Juvenile Rainbow Trout ◽  
Rainbow Trout Oncorhynchus Mykiss

Long-term primary culture of epithelial cells from rainbow trout (Oncorhynchus mykiss) liver

1995 ◽  
Vol 31 (5) ◽  
pp. 367-378 ◽  
Author(s):  
Gary K. Ostrander ◽  
James B. Blair ◽  
Beverly A. Stark ◽  
Garry M. Marley ◽  
Wesley D. Bales ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Epithelial Cells ◽  
Oncorhynchus Mykiss ◽  
Primary Culture ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals First record of Schulmanela petruschewskii Shulman, 1948 (Nematoda: Capillariidae) from cultured Rainbow trout (Oncorhynchus mykiss) in Turkey

2010 ◽  
Vol 47 (4) ◽  
pp. 273-275
Author(s):  
Z. Pekmezci ◽  
S. Umur
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
New Record ◽  
Parasite Species ◽  
Liver Parenchyma ◽  
First Record ◽  
Parasite Fauna ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
First Time ◽  
North Latitude

AbstractThe nematode Schulmanela petruschewskii (Shulman, 1948) was identified during the parasitological examination on the liver parenchyma in one specimens of a cultured rainbow trout (Oncorhynchus mykiss) which reared in Derbent Dam Lake in Samsun, Turkey (41°25′6′’ North latitude, 35°49′52′’ East longitude) in August 2008. This parasite species was not previously reported from Turkey. With the present study we report S. petruschewskii for the first time in Turkey. This specimen which is a parasite of cultured rainbow trout (Oncorhynchus mykiss) is a new record for the Turkish parasite fauna. Original measurements and figures are presented.

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