scholarly journals eDNAir: proof of concept that animal DNA can be collected from air sampling

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11030
Author(s):  
Elizabeth L. Clare ◽  
Chloe K. Economou ◽  
Chris G. Faulkes ◽  
James D. Gilbert ◽  
Frances Bennett ◽  
...  
Keyword(s):  
Sampling Methods ◽  
Air Sampling ◽  
Research Programme ◽  
Environmental Dna ◽  
Aquatic Systems ◽  
Ecological Research ◽  
Proof Of Concept ◽  
Confined Space ◽  
Substantial Interest

Environmental DNA (eDNA) is one of the fastest developing tools for species biomonitoring and ecological research. However, despite substantial interest from research, commercial and regulatory sectors, it has remained primarily a tool for aquatic systems with a small amount of work in substances such as soil, snow and rain. Here we demonstrate that eDNA can be collected from air and used to identify mammals. Our proof of concept successfully demonstrated that eDNA sampled from air contained mixed templates which reflect the species known to be present within a confined space and that this material can be accessed using existing sampling methods. We anticipate this demonstration will initiate a much larger research programme in terrestrial airDNA sampling and that this may rapidly advance biomonitoring approaches. Lastly, we outline these and potential related applications we expect to benefit from this development.

scholarly journals Droplet- Rather than Aerosol-Mediated Dispersion Is the Primary Mechanism of Bacterial Transmission from Contaminated Hand-Washing Sink Traps

2018 ◽  
Vol 85 (2) ◽  
Author(s):  
Shireen M. Kotay ◽  
Rodney M. Donlan ◽  
Christine Ganim ◽  
Katie Barry ◽  
Bryan E. Christensen ◽  
...  
Keyword(s):  
Patient Care ◽  
Sampling Methods ◽  
Fluorescent Protein ◽  
Model Organism ◽  
Air Sampling ◽  
Multidrug Resistant ◽  
Hand Washing ◽  
Content Type ◽  
E Coli ◽  
Green Fluorescent

ABSTRACT An alarming rise in hospital outbreaks implicating hand-washing sinks has led to widespread acknowledgment that sinks are a major reservoir of antibiotic-resistant pathogens in patient care areas. An earlier study using green fluorescent protein (GFP)-expressing Escherichia coli (GFP-E. coli) as a model organism demonstrated dispersal from drain biofilms in contaminated sinks. The present study further characterizes the dispersal of microorganisms from contaminated sinks. Replicate hand-washing sinks were inoculated with GFP-E. coli, and dispersion was measured using qualitative (settle plates) and quantitative (air sampling) methods. Dispersal caused by faucet water was captured with settle plates and air sampling methods when bacteria were present on the drain. In contrast, no dispersal was captured without or in between faucet events, amending an earlier theory that bacteria aerosolize from the P-trap and disperse. Numbers of dispersed GFP-E. coli cells diminished substantially within 30 minutes after faucet usage, suggesting that the organisms were associated with larger droplet-sized particles that are not suspended in the air for long periods. IMPORTANCE Among the possible environmental reservoirs in a patient care environment, sink drains are increasingly recognized as a potential reservoir to hospitalized patients of multidrug-resistant health care-associated pathogens. With increasing antimicrobial resistance limiting therapeutic options for patients, a better understanding of how pathogens disseminate from sink drains is urgently needed. Once this knowledge gap has decreased, interventions can be engineered to decrease or eliminate transmission from hospital sink drains to patients. The current study further defines the mechanisms of transmission for bacteria that colonize sink drains.

Field Comparison of Air Sampling Methods for Monomeric and Polymeric 1,6-Hexamethylene Diisocyanate

2011 ◽  
Vol 8 (3) ◽  
pp. 161-178 ◽  
Author(s):  
Jennifer M. Thomasen ◽  
Kenneth W. Fent ◽  
Carolyn Reeb-Whitaker ◽  
Stephen G. Whittaker ◽  
Leena A. Nylander-French
Keyword(s):  
Sampling Methods ◽  
Air Sampling ◽  
Hexamethylene Diisocyanate

scholarly journals Droplet rather than Aerosol Mediated Dispersion is the Primary Mechanism of Bacterial transmission from Contaminated Hand Washing Sink Traps

2018 ◽  
Author(s):  
Shireen Kotay ◽  
Rodney M. Donlan ◽  
Christine Ganim ◽  
Katie Barry ◽  
Bryan E. Christensen ◽  
...  
Keyword(s):  
Patient Care ◽  
Sampling Methods ◽  
Model Organism ◽  
Air Sampling ◽  
Multidrug Resistant ◽  
Hand Washing ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Potential Reservoir ◽  
Healthcare Associated

ABSTRACTAn alarming rise in hospital outbreaks implicating hand-washing sinks has led to widespread acknowledgement that sinks are a major reservoir of antibiotic resistant pathogens in patient-care areas. An earlier study using a GFP-expressing Escherichia coli (GFP-E. coli) as a model organism demonstrated dispersal from drain biofilm in contaminated sinks. The present study further characterizes the dispersal of microorganisms from contaminated sinks. Replicate hand-washing sinks were inoculated with GFP-E. coli, and dispersion was measured using qualitative (settle plates) and quantitative (air sampling) methods. Dispersal caused by faucet water was captured with settle plates and air sampling methods when bacteria were present on the drain. In contrast, no dispersal was captured without or in between faucet events amending earlier theory that bacteria aerosolize from P-trap and disperse. Numbers of dispersed GFP-E. coli diminished substantially within 30 minutes after faucet usage, suggesting that the organisms were associated with larger droplet-sized particles that are not suspended in the air for long periods.IMPORTANCEAmong the possible environmental reservoirs in a patient care environment, sink drains are increasingly recognized as potential reservoir of multidrug resistant healthcare-associated pathogens to hospitalized patients. With increasing antimicrobial resistance limiting therapeutic options for patients, better understanding of how pathogens disseminate from sink drains is urgently needed. Once this knowledge gap has decreased, interventions can be engineered to decrease or eliminate transmission from hospital sink drains to patients. The current study further defines the mechanisms of transmission for bacteria colonizing sink drains.

scholarly journals Designing environmental DNA surveys in complex aquatic systems: Backpack sampling for rare amphibians in Sierra Nevada meadows

2020 ◽  
Vol 30 (10) ◽  
pp. 1975-1987 ◽  
Author(s):  
Karen L. Pope ◽  
Caren S. Goldberg ◽  
Nicolette L. Nelson ◽  
Adam K. Cummings ◽  
Travis Seaborn ◽  
...  
Keyword(s):  
Sierra Nevada ◽  
Environmental Dna ◽  
Aquatic Systems ◽  
Sierra Nevada Meadows

Generation of Test Atmospheres of Toxic Substances for Evaluation of Air Sampling Methods

1980 ◽  
pp. 1-11 ◽  
Author(s):  
C. CLARINE ANDERSON ◽  
ELLEN C. GUNDERSON ◽  
DALE M. COULSON ◽  
BRUCE GOODWIN ◽  
KENNETH T. MENZIES
Keyword(s):  
Sampling Methods ◽  
Air Sampling ◽  
Toxic Substances

Improved Membrane-filter Air-sampling Methods for Environmental Control

1962 ◽  
Vol 7 (3) ◽  
pp. 185-190
Author(s):  
K. J. Schiager ◽  
T. M. Allen ◽  
S. B. Gerber ◽  
G. E. Kinsella ◽  
F. J. Krupka ◽  
...  
Keyword(s):  
Sampling Methods ◽  
Environmental Control ◽  
Membrane Filter ◽  
Air Sampling

scholarly journals What’s the potential for environmental DNA (eDNA) to assess stygofauna and trogolofauna: pilot studies from Western Australia

2018 ◽  
Vol 1 ◽  
Author(s):  
Nicole White ◽  
Michelle Guzik ◽  
Steven Cooper ◽  
Andrew Austin ◽  
Michael Bunce
Keyword(s):  
Western Australia ◽  
Environmental Dna ◽  
Proof Of Concept ◽  
Field Sampling ◽  
Fecal Matter ◽  
Collection Method ◽  
Pilot Studies ◽  
Community Assemblage ◽  
Reference Databases ◽  
Biological Organisms

Biological organisms living in any environment can expel DNA into their surroundings through fecal matter, mucus, shed skin, gametes, etc. Here we examine the utility of metabarcoding from a variety of environmental DNA (eDNA) substrates collected from the Pilbara region, Western Australia, to assess the feasibility for both stygo- and troglofauna detection. With metabarcoding, we confirm eDNA from both stygo- and troglofauna is detectable via molecules. In addition, our proof-of-concept and validation of using an eDNA approach was confirmed when both traditional morphological and metabarcoding assessments were compared. The metabarcoding results from the eDNA substrates are very encouraging when compared to the results of traditional morphological assessments, although highlighted the need for comprehensive DNA reference databases to be accessible for metabarcoding comparisons in order to obtain species identifications and community assemblage profiles. Furthermore, our results indicate a standardised field sampling collection method is warranted in order to maximise the success of subterranean eDNA detection from environmental substrates. eDNA data collected suggest metabarcoding approaches will become a powerful part of the toolkit to study subterranean fauna.

Sign in / Sign up

Export Citation Format

Share Document