scholarly journals Designing environmental DNA surveys in complex aquatic systems: Backpack sampling for rare amphibians in Sierra Nevada meadows

2020 ◽  
Vol 30 (10) ◽  
pp. 1975-1987 ◽  
Author(s):  
Karen L. Pope ◽  
Caren S. Goldberg ◽  
Nicolette L. Nelson ◽  
Adam K. Cummings ◽  
Travis Seaborn ◽  
...  
Keyword(s):  
Sierra Nevada ◽  
Environmental Dna ◽  
Aquatic Systems ◽  
Sierra Nevada Meadows

scholarly journals Erosion of refugia in the Sierra Nevada meadows network with climate change

Ecosphere ◽  
2017 ◽  
Vol 8 (4) ◽  
Author(s):  
Sean P. Maher ◽  
Toni Lyn Morelli ◽  
Michelle Hershey ◽  
Alan L. Flint ◽  
Lorraine E. Flint ◽  
...  
Keyword(s):  
Climate Change ◽  
Sierra Nevada ◽  
Sierra Nevada Meadows

scholarly journals eDNAir: proof of concept that animal DNA can be collected from air sampling

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11030
Author(s):  
Elizabeth L. Clare ◽  
Chloe K. Economou ◽  
Chris G. Faulkes ◽  
James D. Gilbert ◽  
Frances Bennett ◽  
...  
Keyword(s):  
Sampling Methods ◽  
Air Sampling ◽  
Research Programme ◽  
Environmental Dna ◽  
Aquatic Systems ◽  
Ecological Research ◽  
Proof Of Concept ◽  
Confined Space ◽  
Substantial Interest

Environmental DNA (eDNA) is one of the fastest developing tools for species biomonitoring and ecological research. However, despite substantial interest from research, commercial and regulatory sectors, it has remained primarily a tool for aquatic systems with a small amount of work in substances such as soil, snow and rain. Here we demonstrate that eDNA can be collected from air and used to identify mammals. Our proof of concept successfully demonstrated that eDNA sampled from air contained mixed templates which reflect the species known to be present within a confined space and that this material can be accessed using existing sampling methods. We anticipate this demonstration will initiate a much larger research programme in terrestrial airDNA sampling and that this may rapidly advance biomonitoring approaches. Lastly, we outline these and potential related applications we expect to benefit from this development.

scholarly journals Comparison of materials for rapid passive collection of environmental DNA

2021 ◽  
Author(s):  
Cindy Bessey ◽  
Yuan Gao ◽  
Yen Bach Truong ◽  
Haylea Miller ◽  
Simon Neil Jarman ◽  
...  
Keyword(s):  
Species Richness ◽  
Sampling Method ◽  
Electrospun Nanofibers ◽  
Environmental Dna ◽  
Aquatic Systems ◽  
Biological Surveys ◽  
Biological Matter ◽  
Cotton Fibres ◽  
Hemp Fibres ◽  
Template Dna

Passive collection is an emerging sampling method for environmental DNA (eDNA) in aquatic systems. Passive eDNA collection is inexpensive, efficient, and requires minimal equipment, making it suited to high density sampling and remote deployment. Here, we compare the effectiveness of nine membrane materials for passively collecting fish eDNA from a 3 million litre marine mesocosm. We submerged materials (cellulose, cellulose with 1% and 3% chitosan, cellulose overlayed with electrospun nanofibers and 1% chitosan, cotton fibres, hemp fibres and sponge with either zeolite or active carbon) for intervals between five and 1080 minutes. We show that for most materials, with as little as five minutes submersion, mitochondrial fish eDNA measured with qPCR, and fish species richness measured with metabarcoding, was comparable to that collected by conventional filtering. Furthermore, PCR template DNA concentrations and species richness were generally not improved significantly by longer submersion. Species richness detected for all materials ranged between 11 to 37 species, with a median of 27, which was comparable to the range for filtered eDNA (19-32). Using scanning electron microscopy, we visualised biological matter adhered to the surface of materials, rather than entrapped, with images also revealing a diversity in size and structure of putative eDNA particles. Environmental DNA can be collected rapidly from seawater with a passive approach and using a variety of materials. This will suit cost and time-sensitive biological surveys, and where access to equipment is limited.

scholarly journals Comparison of materials for rapid passive collection of environmental DNA

2021 ◽  
Author(s):  
Cindy Bessey ◽  
Yuan Gao ◽  
Yen Truong ◽  
Haylea Miller ◽  
Simon Jarman ◽  
...  
Keyword(s):  
Species Richness ◽  
Sampling Method ◽  
Electrospun Nanofibers ◽  
Environmental Dna ◽  
Aquatic Systems ◽  
Biological Surveys ◽  
Biological Matter ◽  
Cotton Fibres ◽  
Hemp Fibres ◽  
Template Dna

Passive collection is an emerging sampling method for environmental DNA (eDNA) in aquatic systems. Passive eDNA collection is inexpensive, efficient, and requires minimal equipment, making it suited to high density sampling and remote deployment. Here, we compare the effectiveness of nine membrane materials for passively collecting fish eDNA from a 3 million litre marine mesocosm. We submerged materials (cellulose, cellulose with 1% and 3% chitosan, cellulose overlayed with electrospun nanofibers and 1% chitosan, cotton fibres, hemp fibres and sponge with either zeolite or active carbon) for intervals between five and 1080 minutes. We show that for most materials, with as little as five minutes submersion, mitochondrial fish eDNA measured with qPCR, and fish species richness measured with metabarcoding, was comparable to that collected by conventional filtering. Furthermore, PCR template DNA concentrations and species richness were generally not improved significantly by longer submersion. Species richness detected for all materials ranged between 11 to 37 species, with a median of 27, which was comparable to the range for filtered eDNA (19-32). Using scanning electron microscopy, we visualised biological matter adhered to the surface of materials, rather than entrapped, with images also revealing a diversity in size and structure of putative eDNA particles. Environmental DNA can be collected rapidly from seawater with a passive approach and using a variety of materials. This will suit cost and time-sensitive biological surveys, and where access to equipment is limited.

Herbage Production on High Sierra Nevada Meadows

1967 ◽  
Vol 20 (4) ◽  
pp. 255 ◽  
Author(s):  
H. Reed Sanderson
Keyword(s):  
Sierra Nevada ◽  
Sierra Nevada Meadows

scholarly journals Evaluating Sierra Nevada Yellow‐Legged Frog Distribution Using Environmental DNA

2021 ◽  
Author(s):  
Mallory E. Bedwell ◽  
Kristina V. S. Hopkins ◽  
Colin Dillingham ◽  
Caren S. Goldberg
Keyword(s):  
Sierra Nevada ◽  
Environmental Dna

scholarly journals Stubble height standards for Sierra Nevada meadows can be difficult to meet

2003 ◽  
Vol 57 (2) ◽  
pp. 60-64 ◽  
Author(s):  
David F Lile ◽  
Kenneth W Tate ◽  
Donald L Lancaster ◽  
Betsy M Karle
Keyword(s):  
Sierra Nevada ◽  
Sierra Nevada Meadows

scholarly journals Investigating diversity of pathogenic microbes in commercial bait trade water

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5468 ◽  
Author(s):  
Andrew R. Mahon ◽  
Dean J. Horton ◽  
Deric R. Learman ◽  
Lucas R. Nathan ◽  
Christopher L. Jerde
Keyword(s):  
Great Lakes ◽  
Lake Michigan ◽  
Hypervariable Region ◽  
Environmental Dna ◽  
Taxonomic Diversity ◽  
Aquatic Systems ◽  
Rrna Gene ◽  
Great Lakes Region ◽  
Pathogen Diversity ◽  
Important Pathway

The recreational bait trade is a potential pathway for pathogen introduction and spread when anglers dump bait shop sourced water into aquatic systems. Despite this possibility, and previous recognition of the importance of the bait trade in the spread of aquatic invasive species (AIS), to date there has been no region wide survey documenting pathogens in retail bait shops. In this study, we analyzed 96 environmental DNA samples from retail bait shops around the Great Lakes region to identify pathogens, targeting the V4 hypervariable region of the 16S rRNA gene. Additionally, we used samples from one site in Lake Michigan as a comparison to pathogen diversity and abundance in natural aquatic systems. Our results identified nine different groups of pathogens in the bait shop samples, including those that pose risks to both humans and fish species. Compared to wild sourced samples, the bait shops had higher relative abundance and greater taxonomic diversity. These findings suggest that the bait trade represents a potentially important pathway that could introduce and spread pathogens throughout the Great Lakes region. Improving pathogen screening and angler outreach should be used in combination to aid in preventing the future spread of high risk pathogens.

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