scholarly journals Functional analysis of CgWRKY57 from Cymbidium goeringii in ABA response

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10982
Author(s):  
Huanhuan Liu ◽  
Lianping Wang ◽  
Xijun Jing ◽  
Yue Chen ◽  
Fengrong Hu
Keyword(s):  
Abiotic Stress ◽  
Transgenic Plants ◽  
Real Time ◽  
Growth And Development ◽  
Transmembrane Domain ◽  
Germination Rate ◽  
Wild Type ◽  
Abiotic Stress Response ◽  
Group Iii ◽  
Cymbidium Goeringii

Background The orchid is one of the top ten Chinese flowers and has high ornamental value and elegant color. However, orchids are vulnerable to abiotic stresses during their growth and development, and the molecular mechanism of the abiotic stress response in orchids is unclear. WRKY proteins belong to a transcription factor family that plays important roles in biotic stress, abiotic stress, growth and development in plants, but little is known about the WRKY family in Cymbidium goeringii. Methods The specific fragment of the CgWRKY57 gene of C. goeringii was analyzed by bioinformatics. The expression of the CgWRKY57 gene of C. goeringii under 4 °C, 42 °C water and ABA stress as well as different tissues was detected by real-time fluorescence quantitative PCR. CgWRKY57 gene was overexpressed in wild type Arabidopsis thaliana by inflorescence infection method, and the function of transgenic lines under ABA stress was analyzed. Results CgWRKY57 was cloned from C. goeringii and found to encode 303 amino acids. The CgWRKY57 protein is an acidic, nonsecreted hydrophilic protein without a signal peptide or transmembrane domain. The CgWRKY57 protein is located to the nucleus and may function intracellularly according to its predicted subcellular localization. A domain analysis and homology comparison showed that the CgWRKY57 protein has a “WRKYGQK” domain and belongs to Group III of the WRKY family, and a phylogenetic analysis demonstrated that CgWRKY57 is closely related to OsWRKY47. CgWRKY57 was expressed in the roots, stems, leaves and floral organs of C. goeringii, and its expression level was highest in the roots according to real-time qPCR analysis. There were significant differences in CgWRKY57 expression under 4 °C, 42 °C ABA and water stress treatments, and its expression changed greatly under ABA stress. The expression of CgWRKY57 in transgenic plants was significantly higher than that in wild type plants under ABA stress, and the root length and germination rate were reduced in transgenic plants compared to wild type plants. Conclusions These results indicate that CgWRKY57 overexpression is responsive to ABA stress, and they provide a foundation for future analyses of the biological functions of the WRKY family in C. goeringii.

scholarly journals The BpMYB4 Transcription Factor From Betula platyphylla Contributes Toward Abiotic Stress Resistance and Secondary Cell Wall Biosynthesis

2021 ◽  
Vol 11 ◽  
Author(s):  
Ying Yu ◽  
Huizi Liu ◽  
Nan Zhang ◽  
Caiqiu Gao ◽  
Liwang Qi ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factor ◽  
Abiotic Stress ◽  
Transgenic Plants ◽  
Stress Resistance ◽  
Secondary Cell Wall ◽  
Lignin Content ◽  
Lignin Biosynthesis ◽  
Betula Platyphylla ◽  
Wild Type

The MYB (v-myb avian myeloblastosis viral oncogene homolog) family is one of the largest transcription factor families in plants, and is widely involved in the regulation of plant metabolism. In this study, we show that a MYB4 transcription factor, BpMYB4, identified from birch (Betula platyphylla Suk.) and homologous to EgMYB1 from Eucalyptus robusta Smith and ZmMYB31 from Zea mays L. is involved in secondary cell wall synthesis. The expression level of BpMYB4 was higher in flowers relative to other tissues, and was induced by artificial bending and gravitational stimuli in developing xylem tissues. The expression of this gene was not enriched in the developing xylem during the active season, and showed higher transcript levels in xylem tissues around sprouting and near the dormant period. BpMYB4 also was induced express by abiotic stress. Functional analysis indicated that expression of BpMYB4 in transgenic Arabidopsis (Arabidopsis thaliana) plants could promote the growth of stems, and result in increased number of inflorescence stems and shoots. Anatomical observation of stem sections showed lower lignin deposition, and a chemical contents test also demonstrated increased cellulose and decreased lignin content in the transgenic plants. In addition, treatment with 100 mM NaCl and 200 mM mannitol resulted in the germination rate of the over-expressed lines being higher than that of the wild-type seeds. The proline content in transgenic plants was higher than that in WT, but MDA content was lower than that in WT. Further investigation in birch using transient transformation techniques indicated that overexpression of BpMYB4 could scavenge hydrogen peroxide and O2.– and reduce cell damage, compared with the wild-type plants. Therefore, we believe that BpMYB4 promotes stem development and cellulose biosynthesis as an inhibitor of lignin biosynthesis, and has a function in abiotic stress resistance.

scholarly journals OsCBE1, a Substrate Receptor of Cullin4-Based E3 Ubiquitin Ligase, Functions as a Regulator of Abiotic Stress Response and Productivity in Rice

2021 ◽  
Vol 22 (5) ◽  
pp. 2487
Author(s):  
Juyoung Choi ◽  
Wonkyung Lee ◽  
Gynheung An ◽  
Seong-Ryong Kim
Keyword(s):  
Abiotic Stress ◽  
Stress Response ◽  
Cold Stress ◽  
Stress Tolerance ◽  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
In Silico Analysis ◽  
Wild Type ◽  
Abiotic Stress Response ◽  
Environmental Stress Response

Ubiquitination is an important environmental stress response, and E3 ubiquitin ligases play a major role in the process. T-DNA insertion mutants of rice, Oscbe1-1, and Oscbe1-2, were identified through the screening of cold stress tolerance at seedling stage. Oscbe1 mutants showed a significantly higher cold stress tolerance in the fresh weight, chlorophyll content, and photosynthetic efficiency than wild type. Molecular prediction showed that OsCBE1 (Oryza sativa Cullin4-Based E3 ubiquitin ligase1) encoded a novel substrate receptor of Cullin4-based E3 ubiquitin ligase complex (C4E3). Whereas Oscbe1 mutants had fewer panicles and grains than wild type in the paddy field, the overexpression lines of OsCBE1 had more panicles and grains, suggesting that OsCBE1 is involved in the regulation of both abiotic stress response and development. Oscbe1 mutants also showed ABA hypersensitivity during seed germination, suggesting OsCBE1 function for the stress response via ABA signaling. In silico analysis of OsCBE1 activity predicted a CCCH-type transcription factor, OsC3H32, as a putative substrate. Co-IP (Co-immunoprecipitation) study showed that OsCBE1 interacts with OsDDB1, an expected binding component of OsCBE1 and OsC3H32. Additionally, expression of OsOLE16, OsOLE18, and OsBURP5 were negatively related with expression of OsCBE1. These results suggest that OsCBE1 functions as a regulator of the abiotic stress response via CCCH as a member of the C4E3.

scholarly journals Overexpression of an evolutionarily conserved drought-responsive sugarcane gene enhances salinity and drought resilience

2019 ◽  
Vol 124 (4) ◽  
pp. 691-700 ◽  
Author(s):  
Kevin Begcy ◽  
Eduardo D Mariano ◽  
Carolina G Lembke ◽  
Sonia Marli Zingaretti ◽  
Glaucia M Souza ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Oxidative Damage ◽  
Germination Rate ◽  
Co2 Concentration ◽  
Dry Mass ◽  
Wild Type ◽  
Gas Exchange Parameters ◽  
Physiological Level ◽  
Tobacco Seeds ◽  
Genes Encoding

Abstract Background and Aims Improving drought adaptation is more pressing for crops such as sugarcane, rice, wheat and maize, given the high dependence of these crops on irrigation. One option for enhancing adaptation to water limitation in plants is by transgenic approaches. An increasing number of genes that are associated with mechanisms used by plants to cope with water scarcity have been discovered. Genes encoding proteins with unknown functions comprise a relevant fraction of the genes that are modulated by drought. We characterized a gene in response to environmental stresses to gain insight into the unknown fraction of the sugarcane genome. Scdr2 (Sugarcane drought-responsive 2) encodes a small protein and shares highly conserved sequences within monocots, dicots, algae and fungi. Methods Plants overexpressing the Scdr2 sugarcane gene were examined in response to salinity and drought. Measurements of the gas exchange parameters, germination rate, water content, dry mass and oxidative damage were performed. Seeds as well as juvenile plants were used to explore the resilience level of the transgenic plants when compared with wild-type plants. Key Results Overexpression of Scdr2 enhanced germination rates in tobacco seeds under drought and salinity conditions. Juvenile transgenic plants overexpressing Scdr2 and subjected to drought and salinity stresses showed higher photosynthesis levels, internal CO2 concentration and stomatal conductance, reduced accumulation of hydrogen peroxide in the leaves, no penalty for photosystem II and faster recovery after submission to both stress conditions. Respiration was not strongly affected by both stresses in the Scdr2 transgenic plants, whereas wild-type plants exhibited increased respiration rates. Conclusions Scdr2 is involved in the response mechanism to abiotic stresses. Higher levels of Scdr2 enhanced resilience to salinity and drought, and this protection correlated with reduced oxidative damage. Scdr2 confers, at the physiological level, advantages to climate limitations. Therefore, Scdr2 is a potential target for improving sugarcane resilience to abiotic stress.

scholarly journals Arabidopsis G-Protein β Subunit AGB1 Negatively Regulates DNA Binding of MYB62, a Suppressor in the Gibberellin Pathway

2021 ◽  
Vol 22 (15) ◽  
pp. 8270
Author(s):  
Xin Qi ◽  
Wensi Tang ◽  
Weiwei Li ◽  
Zhang He ◽  
Weiya Xu ◽  
...  
Keyword(s):  
Dna Binding ◽  
Transgenic Plants ◽  
G Protein ◽  
G Proteins ◽  
Growth And Development ◽  
Β Subunit ◽  
Wild Type ◽  
Binding Assays ◽  
Plant Growth And Development ◽  
Qrt Pcr

Plant G proteins are versatile components of transmembrane signaling transduction pathways. The deficient mutant of heterotrimeric G protein leads to defects in plant growth and development, suggesting that it regulates the GA pathway in Arabidopsis. However, the molecular mechanism of G protein regulation of the GA pathway is not understood in plants. In this study, two G protein β subunit (AGB1) mutants, agb1-2 and N692967, were dwarfed after exogenous application of GA3. AGB1 interacts with the DNA-binding domain MYB62, a GA pathway suppressor. Transgenic plants were obtained through overexpression of MYB62 in two backgrounds including the wild-type (MYB62/WT Col-0) and agb1 mutants (MYB62/agb1) in Arabidopsis. Genetic analysis showed that under GA3 treatment, the height of the transgenic plants MYB62/WT and MYB62/agb1 was lower than that of WT. The height of MYB62/agb1 plants was closer to MYB62/WT plants and higher than that of mutants agb1-2 and N692967, suggesting that MYB62 is downstream of AGB1 in the GA pathway. qRT-PCR and competitive DNA binding assays indicated that MYB62 can bind MYB elements in the promoter of GA2ox7, a GA degradation gene, to activate GA2ox7 transcription. AGB1 affected binding of MYB62 on the promoter of GA2ox7, thereby negatively regulating th eactivity of MYB62.

scholarly journals The long non-coding RNA lncRNA973 is involved in cotton response to salt stress

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Xiaopei Zhang ◽  
Jie Dong ◽  
Fenni Deng ◽  
Wei Wang ◽  
Yingying Cheng ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Transgenic Plants ◽  
Germination Rate ◽  
Stress Conditions ◽  
Virus Induced Gene Silencing ◽  
Wild Type ◽  
Rna Molecules ◽  
Regulatory Processes ◽  
Reduced Salt

Abstract Background Long non-coding (lnc) RNAs are a class of functional RNA molecules greater than 200 nucleotides in length, and lncRNAs play important roles in various biological regulatory processes and response to the biotic and abiotic stresses. LncRNAs associated with salt stress in cotton have been identified through RNA sequencing, but the function of lncRNAs has not been reported. We previously identified salt stress-related lncRNAs in cotton (Gossypium spp.), and discovered the salt-related lncRNA-lncRNA973. Results In this study, we identified the expression level, localization, function, and preliminary mechanism of action of lncRNA973. LncRNA973, which was localized in the nucleus, was expressed at a low level under nonstress conditions but can be significantly increased by salt treatments. Here lncRNA973 was transformed into Arabidopsis and overexpressed. Along with the increased expression compared with wild type under salt stress conditions in transgenic plants, the seed germination rate, fresh weights and root lengths of the transgenic plants increased. We also knocked down the expression of lncRNA973 using virus-induced gene silencing technology. The lncRNA973 knockdown plants wilted, and the leaves became yellowed and dropped under salt-stress conditions, indicating that the tolerance to salt stress had decreased compared with wild type. LncRNA973 may be involved in the regulation of reactive oxygen species-scavenging genes, transcription factors and genes involved in salt stress-related processes in response to cotton salt stress. Conclusions LncRNA973 was localized in the nucleus and its expression was increased by salt treatment. The lncRNA973-overexpression lines had increased salt tolerance compared with the wild type, while the lncRNA973 knockdown plants had reduced salt tolerance. LncRNA973 regulated cotton responses to salt stress by modulating the expression of a series of salt stress-related genes. The data provides a basis for further studies on the mechanisms of lncRNA973-associated responses to salt stress in cotton.

Involvement of OpsLTP1 from Opuntia streptacantha in abiotic stress adaptation and lipid metabolism

2019 ◽  
Vol 46 (9) ◽  
pp. 816
Author(s):  
Mario Rojas ◽  
Francisco Jimenez-Bremont ◽  
Claudia Villicaña ◽  
Laura Carreón-Palau ◽  
Bertha Olivia Arredondo-Vega ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Responses ◽  
Germination Rate ◽  
Lower Amount ◽  
Lipid Transfer ◽  
Loss Of Function ◽  
Wild Type ◽  
Biotic And Abiotic Stress ◽  
Gene Encoding

Plant lipid transfer proteins (LTPs) exhibit the ability to transfer lipids between membranes in vitro, and have been implicated in diverse physiological processes associated to plant growth, reproduction, development, biotic and abiotic stress responses. However, their mode of action is not yet fully understood. To explore the functions of the OpsLTP1 gene encoding a LTP from cactus pear Opuntia streptacantha Lem., we generated transgenic Arabidopsis thaliana (L.) Heynh. plants to overexpress OpsLTP1 and contrasted our results with the loss-of-function mutant ltp3 from A. thaliana under abiotic stress conditions. The ltp3 mutant seeds showed impaired germination under salt and osmotic treatments, in contrast to OpsLTP1 overexpressing lines that displayed significant increases in germination rate. Moreover, stress recovery assays showed that ltp3 mutant seedlings were more sensitive to salt and osmotic treatments than wild-type plants suggesting that AtLTP3 is required for stress-induced responses, while the OpsLTP1 overexpressing line showed no significant differences. In addition, OpsLTP1 overexpressing and ltp3 mutant seeds stored lower amount of total lipids compared with wild-type seeds, showing changes primarily on 16C and 18C fatty acids. However, ltp3 mutant also lead changes in lipid profile and no over concrete lipids which may suggest a compensatory activation of other LTPs. Interestingly, linoleic acid (18:2ω6) was consistently increased in neutral, galactoglycerolipids and phosphoglycerolipids of OpsLTP1 overexpressing line indicating a role of OpsLTP1 in the modulation of lipid composition in A. thaliana.

scholarly journals ALKBH10B, an mRNA m6A Demethylase, Modulates ABA Response During Seed Germination in Arabidopsis

2021 ◽  
Vol 12 ◽  
Author(s):  
Jun Tang ◽  
Junbo Yang ◽  
Hongchao Duan ◽  
Guifang Jia
Keyword(s):  
Abscisic Acid ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Stress Response ◽  
Seed Germination ◽  
Chemical Modification ◽  
Aba Signaling ◽  
Wild Type ◽  
Abiotic Stress Response ◽  
Aba Response

As the most abundant and reversible chemical modification in eukaryotic mRNA, the epitranscriptomic mark N6-methyladenine (m6A) regulates plant development and stress response. We have previously characterized that ALKBH10B is an Arabidopsis mRNA m6A demethylase and regulates floral transition. However, it is unclear whether ALKBH10B plays a role in abiotic stress response. Here, we found that the expression of ALKBH10B is increased in response to abscisic acid (ABA), osmotic, and salt stress. The alkbh10b mutants showed hypersensitive to ABA, osmotic, and salt stress during seed germination. Transcriptome analysis revealed that the expression of several ABA response genes is upregulated in alkbh10b-1 than that of wild type, indicating ALKBH10B negatively affects the ABA signaling. Furthermore, m6A sequencing showed that ABA signaling genes, including PYR1, PYL7, PYL9, ABI1, and SnRK2.2 are m6A hypermethylated in alkbh10b-1 after ABA treatment. Taken together, our work demonstrated that ALKBH10B negatively modulates ABA response during seed germination in Arabidopsis.

scholarly journals Heterologous Expression of Poplar WRKY18/35 Paralogs in Arabidopsis Reveals Their Antagonistic Regulation on Pathogen Resistance and Abiotic Stress Tolerance via Variable Hormonal Pathways

2020 ◽  
Vol 21 (15) ◽  
pp. 5440 ◽  
Author(s):  
Li Guo ◽  
Chaofeng Li ◽  
Yuanzhong Jiang ◽  
Keming Luo ◽  
Changzheng Xu
Keyword(s):  
Transcription Factors ◽  
Transgenic Plants ◽  
Abiotic Stress Tolerance ◽  
Germination Rate ◽  
Pathogen Resistance ◽  
Pcr Analysis ◽  
Wild Type ◽  
Biotic And Abiotic Stresses ◽  
Qrt Pcr ◽  
Root Analysis

WRKY transcription factors (WRKY TFs) are one of the largest protein families in plants, and most of them play vital roles in response to biotic and abiotic stresses by regulating related signaling pathways. In this study, we isolated two WRKY TF genes PtrWRKY18 and PtrWRKY35 from Populustrichocarpa and overexpressed them in Arabidopsis. Expression pattern analyses showed that PtrWRKY18 and PtrWRKY35 respond to salicylic acid (SA), methyl JA (MeJA), abscisic acid (ABA), B. cinereal, and P. syringae treatment. The transgenic plants conferred higher B. cinerea tolerance than wild-type (WT) plants, and real-time quantitative (qRT)-PCR assays showed that PR3 and PDF1.2 had higher expression levels in transgenic plants, which was consistent with their tolerance to B. cinereal. The transgenic plants showed lower P. syringae tolerance than WT plants, and qRT-PCR analysis (PR1, PR2, and NPR1) also corresponded to this phenotype. Germination rate and root analysis showed that the transgenic plants are less sensitive to ABA, which leads to the reduced tolerance to osmotic stress and the increase of the death ratio and stomatal aperture. Compared with WT plants, a series of ABA-related genes (RD29A, ABO3, ABI4, ABI5, and DREB1A) were significantly down-regulated in PtrWRKY18 and PtrWRKY35 overexpression plants. All of these results demonstrated that the two WRKY TFs are multifunctional transcription factors in plant resistance.

scholarly journals Function analysis of ZmZHD9, a positive regulator in drought stress response in transgenic maize

2020 ◽  
Author(s):  
Pengyu Zhang ◽  
Li Wei ◽  
Liru Cao ◽  
Xiao Qiu ◽  
Jiaxu Fu ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Function Analysis ◽  
Maize Yield ◽  
Proline Content ◽  
Wild Type ◽  
Abiotic Stress Response ◽  
Mda Content ◽  
Major Factors

Abstract Backgrounds: Drought stress is one of the major factors that affects maize yield. ZF-HD transcription factors have been proved to play pivotal roles in the regulation of plant growth, hormone conduction signaling and abiotic stress response. However, the molecular mechanism of ZmZHD9-mediated drought tolerance is not well understood. Results: In the present study, we analyzed the functions of ZmZHD9, a member of the maize ZF-HD family. ZmZHD9 is predominantly expressed in leaves, and was induced by drought, salinity, high temperature and abscisic acid (ABA). Subcellular localization indicated that ZmZHD9 protein was localized in the nucleus. ZmZHD9-overexpressing plants showed increased tolerance to drought stress compared with wild-type plants, evaluated by higher RWC and proline content, higher SOD and POD activity, lower REL and MDA content in transgenic plants under drought stress. In addition, the expression of six stress-responsive genes were significantly higher in ZmZHD9 transgenic plants than that in wild-type plants under drought stress.Conclusion: These results demonstrate that ZmZHD9 as a stress-responsive transcription factor which plays a positive regulatory role in response to drought.

scholarly journals Genome-Wide Identification and Expression Analysis of JAZ Family Involved in Hormone and Abiotic Stress in Sweet Potato and Its Two Diploid Relatives

2021 ◽  
Vol 22 (18) ◽  
pp. 9786
Author(s):  
Zhengwei Huang ◽  
Zhen Wang ◽  
Xu Li ◽  
Shaozhen He ◽  
Qingchang Liu ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Sweet Potato ◽  
Stress Responses ◽  
Growth And Development ◽  
Ipomoea Batatas ◽  
Interaction Network ◽  
Functional Study ◽  
Abiotic Stress Response ◽  
Ipomoea Trifida ◽  
Ipomoea Triloba

Jasmonate ZIM-domain (JAZ) proteins are key repressors of a jasmonic acid signaling pathway. They play essential roles in the regulation of plant growth and development, as well as environmental stress responses. However, this gene family has not been explored in sweet potato. In this study, we identified 14, 15, and 14 JAZs in cultivated hexaploid sweet potato (Ipomoea batatas, 2n = 6x = 90), and its two diploid relatives Ipomoea trifida (2n = 2x = 30) and Ipomoea triloba (2n = 2x = 30), respectively. These JAZs were divided into five subgroups according to their phylogenetic relationships with Arabidopsis. The protein physiological properties, chromosome localization, phylogenetic relationship, gene structure, promoter cis-elements, protein interaction network, and expression pattern of these 43 JAZs were systematically investigated. The results suggested that there was a differentiation between homologous JAZs, and each JAZ gene played different vital roles in growth and development, hormone crosstalk, and abiotic stress response between sweet potato and its two diploid relatives. Our work provided comprehensive comparison and understanding of the JAZ genes in sweet potato and its two diploid relatives, supplied a theoretical foundation for their functional study, and further facilitated the molecular breeding of sweet potato.

Sign in / Sign up

Export Citation Format

Share Document