scholarly journals Genome-wide identification of Hsp70/110 genes in rainbow trout and their regulated expression in response to heat stress

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10022
Author(s):  
Fang Ma ◽  
Lintong Luo
Keyword(s):  
Heat Stress ◽  
Rainbow Trout ◽  
Gene Family ◽  
Cold Water ◽  
Functional Characterization ◽  
Head Kidney ◽  
Rna Seq ◽  
Systematic Analysis ◽  
Heat Stress Response ◽  
Shock Proteins

Heat shock proteins (Hsps) play an important role in many biological processes. However, as a typical cold water fish, the systematic identification of Hsp70/110 gene family of rainbow trout (Oncorhynchus mykiss) has not been reported, and the role of Hsp70/110 gene in the evolution of rainbow trout has not been described systematically. In this study, bioinformatics methods were used to analyze the Hsp70/110 gene family of rainbow trout. A total of 16 hsp70/110 genes were identified and classified into ten subgroups. The 16 Hsp70/110 genes were all distributed on chromosomes 2, 4, 8 and 13. The molecular weight is ranged from 78.93 to 91.39 kD. Gene structure and motif composition are relatively conserved in each subgroup. According to RNA-seq analysis of rainbow trout liver and head kidney, a total of four out of 16 genes were significantly upregulated in liver under heat stress, and a total of seven out of 16 genes were significantly upregulated in head kidney. RT-qPCR was carried out on these gene, and the result were consistent with those of RNA-seq. The significantly regulated expressions of Hsp70/110 genes under heat stress indicats that Hsp70/110 genes are involved in heat stress response in rainbow trout. This systematic analysis provided valuable information about the diverse roles of Hsp70/110 in the evolution of teleost, which will contribute to the functional characterization of Hsp70/110 genes in further research.

scholarly journals Genome-wide identification and analysis of the heat shock transcription factor family in moso bamboo (Phyllostachys edulis)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bin Huang ◽  
Zhinuo Huang ◽  
Ruifang Ma ◽  
Jialu Chen ◽  
Zhijun Zhang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Heat Shock ◽  
Gene Family ◽  
Regulatory Elements ◽  
Moso Bamboo ◽  
Naphthalene Acetic Acid ◽  
Plant Responses ◽  
Heat Stress Response ◽  
Regulatory Pathways

AbstractHeat shock transcription factors (HSFs) are central elements in the regulatory network that controls plant heat stress response. They are involved in multiple transcriptional regulatory pathways and play important roles in heat stress signaling and responses to a variety of other stresses. We identified 41 members of the HSF gene family in moso bamboo, which were distributed non-uniformly across its 19 chromosomes. Phylogenetic analysis showed that the moso bamboo HSF genes could be divided into three major subfamilies; HSFs from the same subfamily shared relatively conserved gene structures and sequences and encoded similar amino acids. All HSF genes contained HSF signature domains. Subcellular localization prediction indicated that about 80% of the HSF proteins were located in the nucleus, consistent with the results of GO enrichment analysis. A large number of stress response–associated cis-regulatory elements were identified in the HSF upstream promoter sequences. Synteny analysis indicated that the HSFs in the moso bamboo genome had greater collinearity with those of rice and maize than with those of Arabidopsis and pepper. Numerous segmental duplicates were found in the moso bamboo HSF gene family. Transcriptome data indicated that the expression of a number of PeHsfs differed in response to exogenous gibberellin (GA) and naphthalene acetic acid (NAA). A number of HSF genes were highly expressed in the panicles and in young shoots, suggesting that they may have functions in reproductive growth and the early development of rapidly-growing shoots. This study provides fundamental information on members of the bamboo HSF gene family and lays a foundation for further study of their biological functions in the regulation of plant responses to adversity.

RNA-Seq-mediated transcriptomic analysis of heat stress response in a polar Chlorella sp. (Trebouxiophyceae, Chlorophyta)

2018 ◽  
Vol 30 (6) ◽  
pp. 3103-3119 ◽  
Author(s):  
Sze-Wan Poong ◽  
Kok-Keong Lee ◽  
Phaik-Eem Lim ◽  
Tun-Wen Pai ◽  
Chiew-Yen Wong ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Response ◽  
Transcriptomic Analysis ◽  
Rna Seq ◽  
Heat Stress Response ◽  
Chlorella Sp

Transcriptomic responses to heat stress in rainbow trout Oncorhynchus mykiss head kidney

2018 ◽  
Vol 82 ◽  
pp. 32-40 ◽  
Author(s):  
Jinqiang Huang ◽  
Yongjuan Li ◽  
Zhe Liu ◽  
Yujun Kang ◽  
Jianfu Wang
Keyword(s):  
Heat Stress ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Head Kidney ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Transcriptomic Responses

scholarly journals Environment and HSP90 modulate MAPK stomatal developmental pathway

2018 ◽  
Author(s):  
Despina Samakovli ◽  
Tereza Tichá ◽  
Miroslav Ovečka ◽  
Ivan Luptovčiak ◽  
Veronika Zapletalová ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stomatal Density ◽  
Stress Conditions ◽  
Environmental Cues ◽  
Stomatal Development ◽  
Heat Stress Response ◽  
Tightly Coupled ◽  
Tolerance Response ◽  
Nucleocytoplasmic Distribution ◽  
Shock Proteins

ABSTRACTStomatal ontogenesis is a key element of plant adaptation aiming to control photosynthetic efficiency and water management under fluctuating environments 1,2,3. Development of stomata is guided by endogenous and environmental cues and is tightly coupled to overall plant growth 1,2,3. YODA signaling pathway is essential to stomatal lineage specification4,5,6 since it regulates the activities of transcription factors such as SPEECHLESS (SPCH)7,8,9,10. Heat-shock proteins 90 (HSP90s) are evolutionarily conserved molecular chaperones implicated in a broad range of signalling pathways being integrated in interaction networks with client proteins11,12,13,14. Herein, based on genetic, molecular, biochemical, and cell biological evidence we report that heat-stress conditions affect phosphorylation and deactivation of SPCH and modulate stomatal density. We show that genetic and physical interactions between HSP90s and YODA control stomatal patterning, distribution and morphology. We provide solid evidence that HSP90s play a major role in transducing the heat-stress response since they act upstream and downstream of YODA signalling, regulate the activity and nucleocytoplasmic distribution of MAPKs, and the activation of SPCH. Thus, HSPs control the stomatal development both under normal temperature and acute heat-stress conditions. Our results demonstrate that HSP90s couple stomatal formation and patterning to environmental cues providing an adaptive mechanism of heat-stress tolerance response and stomatal formation in Arabidopsis.

scholarly journals Cysteine-Rich Receptor-Like Kinase Gene Family Identification in the Phaseolus Genome and Comparative Analysis of Their Expression Profiles Specific to Mycorrhizal and Rhizobial Symbiosis

Genes ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 59
Author(s):  
Elsa-Herminia Quezada ◽  
Gabriel-Xicoténcatl García ◽  
Manoj-Kumar Arthikala ◽  
Govindappa Melappa ◽  
Miguel Lara ◽  
...  
Keyword(s):  
Gene Family ◽  
Sequence Similarity ◽  
Expression Profiles ◽  
Functional Characterization ◽  
Arbuscular Mycorrhizal ◽  
Comprehensive Analysis ◽  
Biological Processes ◽  
Kinase Gene ◽  
Systematic Analysis ◽  
Rhizobial Symbiosis

Receptor-like kinases (RLKs) are conserved upstream signaling molecules that regulate several biological processes, including plant development and stress adaptation. Cysteine (C)-rich receptor-like kinases (CRKs) are an important class of RLK that play vital roles in disease resistance and cell death in plants. Genome-wide analyses of CRK genes have been carried out in Arabidopsis and rice, while functional characterization of some CRKs has been carried out in wheat and tomato in addition to Arabidopsis. A comprehensive analysis of the CRK gene family in leguminous crops has not yet been conducted, and our understanding of their roles in symbiosis is rather limited. Here, we report the comprehensive analysis of the Phaseolus CRK gene family, including identification, sequence similarity, phylogeny, chromosomal localization, gene structures, transcript expression profiles, and in silico promoter analysis. Forty-six CRK homologs were identified and phylogenetically clustered into five groups. Expression analysis suggests that PvCRK genes are differentially expressed in both vegetative and reproductive tissues. Further, transcriptomic analysis revealed that shared and unique CRK genes were upregulated during arbuscular mycorrhizal and rhizobial symbiosis. Overall, the systematic analysis of the PvCRK gene family provides valuable information for further studies on the biological roles of CRKs in various Phaseolus tissues during diverse biological processes, including Phaseolus-mycorrhiza/rhizobia symbiosis.

Sign in / Sign up

Export Citation Format

Share Document