Effect of Storage Temperatures And Duration of Storage on Urinary Albumin Concentration in Diabetics

2011 ◽  
Vol 2 (1) ◽  
Author(s):  
Karthik N Rao ◽  
Krishnamurthy U ◽  
Vasudha KC
Keyword(s):  
Urinary Albumin ◽  
Albumin Concentration ◽  
Duration Of Storage ◽  
Storage Temperatures ◽  
Urinary Albumin Concentration

scholarly journals Characterization of Immunochemically Nonreactive Urinary Albumin

2004 ◽  
Vol 50 (12) ◽  
pp. 2286-2291 ◽  
Author(s):  
Tanya M Osicka ◽  
Wayne D Comper
Keyword(s):  
Disulfide Bonds ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Clinical Importance ◽  
Urinary Albumin ◽  
Albumin Concentration ◽  
Urinary Proteins ◽  
Native Page ◽  
Sds Page ◽  
Urinary Albumin Concentration

Abstract Background: Conventional immunoassays underestimate the urinary albumin concentration because intact albumin in urine exists in two forms, immunoreactive and immunochemically nonreactive. Methods: Urinary albumin concentration measured by HPLC (which measures total albumin, i.e., the sum of immunoreactive albumin + immunochemically nonreactive albumin) or RIA was compared with densitometric analysis of albumin bands in diabetic urine samples separated by either native polyacrylamide gel electrophoresis (PAGE) or reducing sodium dodecyl sulfate (SDS)-PAGE. Immunochemically nonreactive albumin was also isolated from diabetic urine (relative amount detected, 70–80% of the expected) and was tested for contamination by common urinary proteins by native PAGE, ELISA, and capillary electrophoresis. Results: Urinary albumin concentrations measured by native PAGE and HPLC were better correlated (r2 = 0.83) than concentrations measured by native PAGE and RIA (r2 = 0.62) because under native conditions both native PAGE and HPLC detect total albumin and not only the immunoreactive albumin alone that is measured by RIA. Urinary albumin concentrations measured by reducing SDS-PAGE and RIA were better correlated (r2 = 0.84) than concentrations measured by reducing SDS-PAGE and HPLC (r2 = 0.65) because under reducing conditions immunochemically nonreactive albumin is unstable and fragments into many smaller peptides. The partially purified preparation was found to contain <1% contamination by common urinary proteins and is stable to freezing and frequent freeze/thaw cycles. Conclusions: The results are consistent with the interpretation that immunochemically nonreactive albumin has a limited number of polypeptide chain scissions and is held together by noncovalent intrachain bonding and disulfide bonds. Detection of this molecule is likely to be of clinical importance in diagnosing kidney disease as well as cardiovascular disease.

Is a random urinary albumin concentration a useful screening test in insulin-treated diabetic patients?

1994 ◽  
Vol 163 (9) ◽  
pp. 406-409 ◽  
Author(s):  
O. L. Beatty ◽  
C. M. Ritchie ◽  
D. R. Hadden ◽  
L. Kennedy ◽  
P. M. Bell ◽  
...  
Keyword(s):  
Screening Test ◽  
Urinary Albumin ◽  
Diabetic Patients ◽  
Albumin Concentration ◽  
Urinary Albumin Concentration

scholarly journals Plasma creatine concentration is associated with incident hypertension in a cohort enriched for the presence of high urinary albumin concentration

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Adrian Post ◽  
Daan Kremer ◽  
J. Casper Swarte ◽  
Sara Sokooti ◽  
Fabian A. Vogelpohl ◽  
...  
Keyword(s):  
Urinary Albumin ◽  
Albumin Concentration ◽  
Incident Hypertension ◽  
Urinary Albumin Concentration

scholarly journals Association of uric acid with kidney function and albuminuria: the Uric Acid Right for heArt Health (URRAH) Project

2021 ◽  
Author(s):  
Elisa Russo ◽  
◽  
Francesca Viazzi ◽  
Roberto Pontremoli ◽  
Carlo Maria Barbagallo ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Uric Acid ◽  
Kidney Disease ◽  
Serum Uric Acid ◽  
Urinary Albumin ◽  
Albumin Concentration ◽  
Heart Health ◽  
The Relationship ◽  
Urinary Albumin Concentration ◽  
Shed Light

Abstract Background Hyperuricemia is commonly observed in patients with chronic kidney disease (CKD). However, a better understanding of the relationship among uric acid (UA) values, glomerular filtration rate (GFR) and albuminuria may shed light on the mechanisms underlying the excess of cardiovascular mortality associated with both chronic kidney disease and hyperuricemia and lead to better risk stratification. Our main goal was to study the relationships between serum uric acid and kidney disease measures (namely estimated GFR [eGFR] and albuminuria) in a large cohort of individuals at cardiovascular risk from the URic acid Right for heArt Health (URRAH) Project database. Methods Clinical data of 26,971 individuals were analyzed. Factors associated with the presence of hyperuricemia defined on the basis of previously determined URRAH cutoffs for cardiovascular and all-cause mortality were evaluated through multivariate analysis. Chronic kidney disease was defined as eGFR < 60 ml/min per 1.73 m2 and/or abnormal urinary albumin excretion diagnosed as: (i) microalbuminuria if urinary albumin concentration was > 30 and ≤ 300 mg/L, or if urinary albumin-to-creatinine ratio (ACR) was > 3.4 mg/mmol and ≤ 34 mg/mmol; (ii) macroalbuminuria if urinary albumin concentration was > 300 mg/L, or if ACR was > 34 mg/mmol. Results Mean age was 58 ± 15 years (51% males, 62% with hypertension and 12% with diabetes), mean eGFR was 81 ml/min per 1.73m22with a prevalence of eGFR < 60 and micro- or macroalbuminuria of 16, 15 and 4%, respectively. Serum uric acid showed a trend towards higher values along with decreasing renal function. Both the prevalence of gout and the frequency of allopurinol use increased significantly with the reduction of eGFR and the increase in albuminuria. Hyperuricemia was independently related to male gender, eGFR strata, and signs of insulin resistance such as body mass index (BMI) and triglycerides. Conclusions The lower the eGFR the higher the prevalence of hyperuricemia and gout. In subjects with eGFR < 60 ml/min the occurrence of hyperuricemia is about 10 times higher than in those with eGFR > 90 ml/min. The percentage of individuals treated with allopurinol was below 2% when GFR was above 60 ml/min, it increased to 20% in the presence of CKD 3b and rose further to 35% in individuals with macroalbuminuria. Graphic abstract

scholarly journals Prognostic value of urinary albumin concentration in diabetic patients surviving the first 5 years after assessment

Diabetologia ◽  
1994 ◽  
Vol 37 (12) ◽  
pp. 1287-1288 ◽  
Author(s):  
H. A. W. Neil ◽  
M. M. Hawkins ◽  
M. H. N. Potock ◽  
J. I. Mann
Keyword(s):  
Prognostic Value ◽  
Urinary Albumin ◽  
Diabetic Patients ◽  
Albumin Concentration ◽  
Urinary Albumin Concentration
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