Is a random urinary albumin concentration a useful screening test in insulin-treated diabetic patients?

1994 ◽  
Vol 163 (9) ◽  
pp. 406-409 ◽  
Author(s):  
O. L. Beatty ◽  
C. M. Ritchie ◽  
D. R. Hadden ◽  
L. Kennedy ◽  
P. M. Bell ◽  
...  
Keyword(s):  
Screening Test ◽  
Urinary Albumin ◽  
Diabetic Patients ◽  
Albumin Concentration ◽  
Urinary Albumin Concentration

scholarly journals Prognostic value of urinary albumin concentration in diabetic patients surviving the first 5 years after assessment

Diabetologia ◽  
1994 ◽  
Vol 37 (12) ◽  
pp. 1287-1288 ◽  
Author(s):  
H. A. W. Neil ◽  
M. M. Hawkins ◽  
M. H. N. Potock ◽  
J. I. Mann
Keyword(s):  
Prognostic Value ◽  
Urinary Albumin ◽  
Diabetic Patients ◽  
Albumin Concentration ◽  
Urinary Albumin Concentration

Screening for microalbuminuria in patients with diabetes mellitus: frozen storage of urine samples decreases their albumin content.

1989 ◽  
Vol 35 (2) ◽  
pp. 308-310 ◽  
Author(s):  
L D Elving ◽  
J A Bakkeren ◽  
M J Jansen ◽  
C M de Kat Angelino ◽  
E de Nobel ◽  
...  
Keyword(s):  
Frozen Storage ◽  
Urine Samples ◽  
Diabetic Patients ◽  
Albumin Concentration ◽  
Low Concentrations ◽  
Albumin Content ◽  
Patients With Diabetes ◽  
The Difference ◽  
Urinary Albumin Concentration

Abstract The influence of storage on urinary albumin concentration was prospectively studied with use of overnight urine specimens (Albustix negative) from 73 diabetic patients. From each urine sample four aliquots were taken. One was stored at 4 degrees C and assayed within two weeks, the other three were stored at -20 degrees C and assayed within two weeks and after two and six months. Albumin concentration was measured with laser immunonephelometry. The detection limit, 1 mg/L, suffices for the screening of diabetic patients for microalbuminuria. After storage for two and six months at -20 degrees C, significantly lower albumin concentrations were found. The difference was mainly caused by lower concentrations found in urine samples in which a precipitate had formed, which was the case in 22 and 25 samples, respectively. Thus, freezing of urine samples for determination of low concentrations of albumin may yield falsely low results. Urine samples are best stored at 4 degrees C and assayed within two weeks.

scholarly journals Characterization of Immunochemically Nonreactive Urinary Albumin

2004 ◽  
Vol 50 (12) ◽  
pp. 2286-2291 ◽  
Author(s):  
Tanya M Osicka ◽  
Wayne D Comper
Keyword(s):  
Disulfide Bonds ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Clinical Importance ◽  
Urinary Albumin ◽  
Albumin Concentration ◽  
Urinary Proteins ◽  
Native Page ◽  
Sds Page ◽  
Urinary Albumin Concentration

Abstract Background: Conventional immunoassays underestimate the urinary albumin concentration because intact albumin in urine exists in two forms, immunoreactive and immunochemically nonreactive. Methods: Urinary albumin concentration measured by HPLC (which measures total albumin, i.e., the sum of immunoreactive albumin + immunochemically nonreactive albumin) or RIA was compared with densitometric analysis of albumin bands in diabetic urine samples separated by either native polyacrylamide gel electrophoresis (PAGE) or reducing sodium dodecyl sulfate (SDS)-PAGE. Immunochemically nonreactive albumin was also isolated from diabetic urine (relative amount detected, 70–80% of the expected) and was tested for contamination by common urinary proteins by native PAGE, ELISA, and capillary electrophoresis. Results: Urinary albumin concentrations measured by native PAGE and HPLC were better correlated (r2 = 0.83) than concentrations measured by native PAGE and RIA (r2 = 0.62) because under native conditions both native PAGE and HPLC detect total albumin and not only the immunoreactive albumin alone that is measured by RIA. Urinary albumin concentrations measured by reducing SDS-PAGE and RIA were better correlated (r2 = 0.84) than concentrations measured by reducing SDS-PAGE and HPLC (r2 = 0.65) because under reducing conditions immunochemically nonreactive albumin is unstable and fragments into many smaller peptides. The partially purified preparation was found to contain <1% contamination by common urinary proteins and is stable to freezing and frequent freeze/thaw cycles. Conclusions: The results are consistent with the interpretation that immunochemically nonreactive albumin has a limited number of polypeptide chain scissions and is held together by noncovalent intrachain bonding and disulfide bonds. Detection of this molecule is likely to be of clinical importance in diagnosing kidney disease as well as cardiovascular disease.

scholarly journals Plasma creatine concentration is associated with incident hypertension in a cohort enriched for the presence of high urinary albumin concentration

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Adrian Post ◽  
Daan Kremer ◽  
J. Casper Swarte ◽  
Sara Sokooti ◽  
Fabian A. Vogelpohl ◽  
...  
Keyword(s):  
Urinary Albumin ◽  
Albumin Concentration ◽  
Incident Hypertension ◽  
Urinary Albumin Concentration

scholarly journals Assessing albuminuria in spot morning samples from diabetic patients

2008 ◽  
Vol 52 (9) ◽  
pp. 1482-1488 ◽  
Author(s):  
Emerson Sampaio ◽  
Vinicius Daher Alvares Delfino
Keyword(s):  
Roc Curves ◽  
Cross Sectional Study ◽  
Urinary Albumin ◽  
Diabetic Patients ◽  
Albumin Concentration ◽  
Cross Sectional ◽  
Incipient Nephropathy ◽  
Similar Accuracy

Microalbuminuria assessment is essential for diagnosing incipient nephropathy in diabetic patients. The present study aim to evaluate whether urinary albumin concentration (UAC) and urinary albumin-to-creatinine ratio (UACR) agree with 24 h urine collection in screening for albuminuria > 30 mg/24 h in type 1 and 2 diabetics. In this cross-sectional study were evaluated 293 diabetic patients (117 type 1 and 176 type 2). Albuminuria was determinated by turbidimetric immunoassay. The best discriminator value was 22 mg/l (sensitivity 82.5%, specificity 74.0%) for UAC and 27.3 mg/g creatinine (sensitivity 83.3%, specificity 80.9%) for UACR. Areas under ROC curves were 0.868 and 0.878, respectively (p = 0.53). Lower discriminators as 10 mg/l (sensitivity 94.2%, specificity 48.6%) and 10 mg/g creatinine (sensitivity 96.7%, specificity 49.1%) attained high sensitivities. UAC and UACR from spot morning urine had similar accuracy in screening microalbuminuria. The simplicity and lower cost of UAC justifies its preferential clinical use.

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