scholarly journals Isolation of Bacillus spp. including B. thuringiensis from apparently healthy female Aedes vexans nipponii

1994 ◽  
Vol 45 (3) ◽  
pp. 233-237
Author(s):  
Shogo MASUDA ◽  
Hiroko JITSUKAWA ◽  
Keiko SEKI ◽  
Junji SAKURADA ◽  
Miyo MURAI ◽  
...  
1968 ◽  
Vol 46 (5) ◽  
pp. 1077-1079 ◽  
Author(s):  
Milan Trpiš ◽  
W. O. Haufe ◽  
J. A. Shemanchuk

Newly emerged and apparently healthy female adults of Aedes vexans Meigen were collected in the Fraser Valley, British Columbia, After they had fed once on man at the time of collection and daily on guinea pigs thereafter in the laboratory, they were found to be infected with mermithid worms that developed rapidly at 25 °C. The parasites suppressed ovarian development and caused nearly 100% mortality within 10 days. They started to leave morbid hosts within 7 days. More detailed studies of the life cycle of the mermithid parasites are needed to assess their effectiveness and specificity in the biological control of A. vexans.


Author(s):  
G. Vijayakumar ◽  
K. Sasikala ◽  
S. Sivaraman ◽  
G. A. Balasubramaniam

Twenty apparently healthy female cattle were selected for the study. Animals were withdrawn of feed and water for 48 hours. During this period, cattle were administered with dextrose normal saline (@10ml/ kg IV twice daily) and were subjected to endoscopy every 12 hours. Animals were restrained in a chute and no sedation was followed. Endoscopy was performed using Olympus™ [GIF V70] flexible video endoscope. The endoscope was passed through the ventral nasal meatus, the pharynx and oesophagus and then into the reticulo-rumen. The entire rumen could be visualized clearly only when the animals were off feed and water for at least 36-48 hours and in some animals the ingesta had to be removed by lavage. The rumen appeared as pink with numerous papillae throughout its surface with different shapes. The procedure was well tolerated by all the cattle and satisfactory ruminal images could be obtained including biopsy.


2020 ◽  
Vol 44 (4) ◽  
pp. 81-90
Author(s):  
F. O. Olufemi ◽  
O. B. Keinde ◽  
P. A. Akinduti ◽  
O. A Odunfa

Studies conducted on the bacterial flora of the respiratory tract in goats or sheep focused on the clinically ill, with fewer studies on the apparently healthy goats and sheep and the antibiogram of isolated organisms. This study was carried out on apparently healthy 54 goats and 43 sheep. A total of seven different bacterial species were isolated from the upper respiratory tracts of the apparently healthy small ruminants using colonial morphology, gram staining and biochemical characterization.Antibiotic sensitivity of the bacterial isolates was performed against 10 commonly prescribed antimicrobial agents and minimum inhibitory concentration (MIC) of the antibiotics was conducted. The overall occurrence rate of bacteria isolated are Pseudomonas spp (42.0% in caprine and 27.3% in ovine); Bacillus spp (36.9%, caprine; 40%, ovine); Mannheima spp, (9.2%, caprine; 23.6%, ovine); Escherichia coli (7.6%, caprine; 9.1%, ovine); Staphylococcus spp (2.5%, caprine); Pasteurellaspp(0.8%, caprine) and Streptococcus spp (0.8%, caprine). The isolation of Pasteurella multocida and Mannheimia haemolytica from the nasal cavity of apparently healthy goats and sheep in this study reflects their possible role in most common respiratory diseases encountered in these small ruminants. All the 174 (100%) isolates were resistant to Amoxicillin and 161 (92.5%) were resistant to Ceftriaxone. One hundred and sixty-eight (96.6%) isolates were sensitive to Ofloxacin and 140 (80.5%) were sensitive to both Gentamycin and Ciprofloxacin and 135 (77.6%) were sensitive to Perfloxin. Staphaureus was resistant to all the antibiotics used except Amoxicillin hence only Amoxicillin can be used for its treatment, while most isolates were susceptible to the antimicrobials tested, as demonstrated by higher MIC value. The emergence of antibiotic resistance to these pathogens  may increase infectious disease burdens and make the rapeutic treatment more expensive.


2019 ◽  
Vol 10 (02) ◽  
pp. 371-373
Author(s):  
Navneet Agarwal ◽  
S. K. Jain ◽  
Vinod Sharma ◽  
Shashi Singhvi ◽  
Sapna Gandhi

ABSTRACTChoriocarcinoma is an uncommon tumor. Undiagnosed choriocarcinoma initially presenting with intracranial hemorrhage in an apparently healthy female is rare and presents a diagnostic difficulty. The authors report a case presenting as intracranial hemorrhage of unsuspected choriocarcinoma. As the measurement of beta-human chorionic gonadotropin (β-hCG) would have helped in the preoperative diagnosis, estimation of serum β-hCG levels is suggested in the diagnostic workup of a female of reproductive age group when radiological investigations are inconclusive. Craniotomy and excision of tumor followed by chemotherapy and radiotherapy may improve the outcome.


2012 ◽  
Vol 28 (1) ◽  
pp. 19-23
Author(s):  
Azizun Nesa ◽  
S D Joy ◽  
M O Faruk

The aim of this study was to isolate and identify uterine microorganisms in postpartum cows and to observe  the occurrence of uterine infection in crossbred and local cows. One hundred and nine samples, from both  crossbred and local dairy cows were collected from Chittagong area. They were inseminated artificially  (n=89) and naturally (n=20). Samples were collected at least 3 months after calving. Among the selected cows,  33 had different reproductive diseases and the rest were from apparently healthy cows. The crossbred dairy  cows were more susceptible to reproductive diseases (19.27%) than the local ones. Uterine infection was  higher (52%) in cows inseminated artificially than that of natural ones (21.21%). Nineteen different types of  bacteria were isolated from both diseased and healthy animals, except Clostridium spp and Nocardia spp which  were not found in diseased animal. Likewise, Bacillus spp and Salmonella spp were absent in healthy animals.  Most prevalent bacteria in diseased animal were Staphylococcus spp and Streptococcus spp found in 33% and  27.3% of the cases respectively. In non-diseased cases, the highest number of samples was positive for  Bacteroied spp (13.2%) followed by Streptococcus spp (10.4%). On average, the highest number of bacterial  population was isolated from diseased samples and most of those bacteria are pathogenic. DOI: http://dx.doi.org/10.3329/bjm.v28i1.11804 Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 19-23


2019 ◽  
Vol 33 (S1) ◽  
Author(s):  
Marcelo Pena ◽  
Francisco J Pena ◽  
Lisa Rodriguez ◽  
Francisco J Morales‐Acuna ◽  
Alvaro N Gurovich

2014 ◽  
Vol 8 (07) ◽  
pp. 909-913 ◽  
Author(s):  
Gamal Wareth ◽  
Jayaseelan Murugaiyan ◽  
Dalia F Khater ◽  
Shawky Ahmed Moustafa

Introduction: Camels migrate between the open boundaries of Sudan and Egypt either for grazing or for slaughtering. Bad hygiene and stress is often related to pulmonary diseases in camels. This study investigated whether camels slaughtered in Cairo carried pulmonary infections. Methodology: Five hundred lung tissues of slaughtered camels were examined and 100 samples suspected for pulmonary infection were subjected to microbial identification and histopathology. Results: A total of 70 lung tissues revealed 97 bacterial isolates of 8 species, including Staphylococcus aureus (37.14%), Escherichia coli (27.14%), Klebsiella pneumoniae (26.71%), Bacillus spp. (25.72%), Streptococcus pyogenes (10%), Corynebacterium spp. (8.85 %), Pasteurella spp. (2.85%), and Arcanobacterium pyogenes (1.4%). Some of these species were earlier reported to be associated with pulmonary infection. Histopathology revealed different types of pneumonia in 50% of the investigated lungs. Conclusions: A considerable number of apparently healthy camels carry pathogenic agents in their lower respiratory tracts. Immunosuppression and stressful conditions might influence these pathogens to induce respiratory diseases in camels. Thus, the infected camels might act as reservoir of these infections agents. If adequate care is not taken, this might be a threat to abattoir workers and may spread infections to humans.


2013 ◽  
Vol 1 (2) ◽  
pp. 46-49 ◽  
Author(s):  
Shobnom Sultana ◽  
Md Ariful Islam ◽  
Mst. Minara Khatun ◽  
Shamima Nasrin

The study was undertaken for isolation, identification and in vitro antibiotic sensitivity assay of bacteria present in the respiratory tract of apparently healthy Japanese quails. A total of 50 samples comprised of tracheal swabs (n = 18), tracheal washings (n = 8), air sacs (n = 8), lungs (n = 8) and exudates of infraorbital sinuses (n = 8) were aseptically collected from 26 apparently healthy Japanese quails. The samples were inoculated onto a variety of media for isolation of bacteria. Identification of bacteria was performed by colony morphology, Gram’s staining and biochemical tests. In total, 25 (50%), 9 (18%), 22 (44%), 20 (40%) and 24 (48%) isolates were identified as Escherichia coli, Salmonella spp., Pasteurella spp., Bacillus spp. and Staphylococcus spp. respectively. Antibiotic sensitivity tests of one randomly selected isolate from each genus of bacteria were performed against 10 common antibiotics. E. coli showed resistance to four antibiotics (amoxicillin, gentamycin, nalidixic acid and tetracycline); Salmonella spp. showed resistance to seven antibiotics (amoxicillin, ampicillin, erythromycin, gentamycin, kanamycin, nalidixic acid and tetracycline); Pasteurella spp. to three antibiotics (erythromycin, sulphamethoxazole and tetracycline); Bacillus spp. to chloramphenicol and nalidixic acid and Staphylococcus spp. to amoxicillin and ampicillin. All the isolates were susceptible to ciprofloxacin. Data of this study indicate that multidrug resistant bacteria are present in the respiratory tract of clinically healthy quails. This is the first report of isolation, identification and antibiogram profile of bacteria present in the respiratory tract of quails in Bangladesh. DOI: http://dx.doi.org/10.3329/mh.v1i2.14088 Microbes and Health, 2012 1(2): 46-49


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