Research of Bulk Erase Operation in Vertical Three-Dimensional Cell Array Architecture

2013 ◽  
Vol 52 (4S) ◽  
pp. 04CD14
Author(s):  
Hyung-jun Yang ◽  
Gae-hun Lee ◽  
Kyeong-rok Kim ◽  
Yun-heub Song
Keyword(s):  
Three Dimensional ◽  
Cell Array ◽  
Array Architecture ◽  
Dimensional Cell

Modeling and experimental verification on directivity of an electret cell array loudspeaker

2012 ◽  
Vol 24 (3) ◽  
pp. 326-333 ◽  
Author(s):  
Yu-Chi Chen ◽  
Wen-Ching Ko ◽  
Han-Lung Chen ◽  
Hsu-Ching Liao ◽  
Wen-Jong Wu ◽  
...  
Keyword(s):  
Finite Element Analysis ◽  
Finite Element ◽  
Three Dimensional ◽  
Digital Control System ◽  
Analysis Model ◽  
Cell Array ◽  
Element Analysis ◽  
Finite Element Analysis Model ◽  
The Finite Element Analysis ◽  
Directivity Characteristics

We propose a model to give us a method to investigate the characteristic three-dimensional directivity in an arbitrarily configured flexible electret-based loudspeaker. In recent years, novel electret loudspeakers have attracted much interest due to their being lightweight, paper thin, and possessing excellent mid- to high-frequency responses. Increasing or decreasing the directivity of an electret loudspeaker makes it excellent for adoption to many applications, especially for directing sound to a particular area or specific audio location. Herein, we detail a novel electret loudspeaker that possesses various directivities and is based on various structures of spacers instead of having to use multichannel amplifiers and a complicated digital control system. In order to study the directivity of an electret loudspeaker based on an array structure which can be adopted for various applications, the horizontal and vertical polar directivity characteristics as a function of frequency were simulated by a finite-element analysis model. To validate the finite-element analysis model, the beam pattern of the electret loudspeaker was measured in an anechoic room. Both the simulated and experimental results are detailed in this article to validate the various assertions related to the directivity of electret cell-based smart speakers.

scholarly journals Can keratin scaffolds be used for creating three-dimensional cell cultures?

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Chemical Activation ◽  
Three Dimensional ◽  
Enzymatic Digestion ◽  
Pepsin Digestion ◽  
Cell Culturing ◽  
3D Cell Cultures ◽  
Associated Proteins ◽  
Positive Effect ◽  
Dimensional Cell

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.

scholarly journals Factors to consider when interrogating 3D culture models with plate readers or automated microscopes

2021 ◽  
Author(s):  
Terry Riss ◽  
O. Joseph Trask
Keyword(s):  
Cell Culture ◽  
Three Dimensional ◽  
3D Culture ◽  
Fluorescent Imaging ◽  
Culture Models ◽  
Assay Methods ◽  
Diffusion Rates ◽  
Cell Culture Models ◽  
Dimensional Cell ◽  
Cells Cultured

AbstractAlong with the increased use of more physiologically relevant three-dimensional cell culture models comes the responsibility of researchers to validate new assay methods that measure events in structures that are physically larger and more complex compared to monolayers of cells. It should not be assumed that assays designed using monolayers of cells will work for cells cultured as larger three-dimensional masses. The size and barriers for penetration of molecules through the layers of cells result in a different microenvironment for the cells in the outer layer compared to the center of three-dimensional structures. Diffusion rates for nutrients and oxygen may limit metabolic activity which is often measured as a marker for cell viability. For assays that lyse cells, the penetration of reagents to achieve uniform cell lysis must be considered. For live cell fluorescent imaging assays, the diffusion of fluorescent probes and penetration of photons of light for probe excitation and fluorescent emission must be considered. This review will provide an overview of factors to consider when implementing assays to interrogate three dimensional cell culture models.

scholarly journals Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment

2017 ◽  
Vol 117 (20) ◽  
pp. 12764-12850 ◽  
Author(s):  
Guoyou Huang ◽  
Fei Li ◽  
Xin Zhao ◽  
Yufei Ma ◽  
Yuhui Li ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Biomimetic Materials ◽  
Cell Microenvironment ◽  
Dimensional Cell
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