Polysaccharide Functionalized Magnetic Nanoparticles for Cell Labeling and Tracking: A New Three-Dimensional Cell-Array System for Toxicity Testing

Author(s):  
Y. Miyamoto ◽  
Y. Koshidaka ◽  
H. Noguchi ◽  
K. Oishi ◽  
H. Saito ◽  
...  
Biomaterials ◽  
2013 ◽  
Vol 34 (34) ◽  
pp. 8555-8563 ◽  
Author(s):  
Jeong Ah Kim ◽  
Jong-Ho Choi ◽  
Minsoo Kim ◽  
Won Jong Rhee ◽  
Boram Son ◽  
...  

2013 ◽  
Vol 52 (4S) ◽  
pp. 04CD14
Author(s):  
Hyung-jun Yang ◽  
Gae-hun Lee ◽  
Kyeong-rok Kim ◽  
Yun-heub Song

2015 ◽  
Vol 21 (42) ◽  
pp. 6038-6054 ◽  
Author(s):  
Dragoș Gudovan ◽  
Paul Balaure ◽  
Dan Mihăiescu ◽  
Adrian Fudulu ◽  
Bogdan Purcăreanu ◽  
...  

2012 ◽  
Vol 24 (3) ◽  
pp. 326-333 ◽  
Author(s):  
Yu-Chi Chen ◽  
Wen-Ching Ko ◽  
Han-Lung Chen ◽  
Hsu-Ching Liao ◽  
Wen-Jong Wu ◽  
...  

We propose a model to give us a method to investigate the characteristic three-dimensional directivity in an arbitrarily configured flexible electret-based loudspeaker. In recent years, novel electret loudspeakers have attracted much interest due to their being lightweight, paper thin, and possessing excellent mid- to high-frequency responses. Increasing or decreasing the directivity of an electret loudspeaker makes it excellent for adoption to many applications, especially for directing sound to a particular area or specific audio location. Herein, we detail a novel electret loudspeaker that possesses various directivities and is based on various structures of spacers instead of having to use multichannel amplifiers and a complicated digital control system. In order to study the directivity of an electret loudspeaker based on an array structure which can be adopted for various applications, the horizontal and vertical polar directivity characteristics as a function of frequency were simulated by a finite-element analysis model. To validate the finite-element analysis model, the beam pattern of the electret loudspeaker was measured in an anechoic room. Both the simulated and experimental results are detailed in this article to validate the various assertions related to the directivity of electret cell-based smart speakers.


Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 249-253
Author(s):  
Marta Bochynska-Czyz ◽  
Patrycja Redkiewicz ◽  
Hanna Kozlowska ◽  
Joanna Matalinska ◽  
Marek Konop ◽  
...  

AbstractThree-dimensional (3D) cell cultures were created with the use of fur keratin associated proteins (F-KAPs) as scaffolds. The procedure of preparation F-KAP involves combinations of chemical activation and enzymatic digestion. The best result in porosity and heterogeneity of F-KAP surface was received during pepsin digestion. The F-KAP had a stable structure, no changes were observed after heat treatment, shaking and washing. The 0.15-0.5 mm fraction had positive effect for formation of 3D scaffolds and cell culturing. Living rat mesenchymal cells on the F-KAP with no abnormal morphology were observed by SEM during 32 days of cell culturing.


1993 ◽  
Vol 21 (2) ◽  
pp. 191-195 ◽  
Author(s):  
Knut-Jan Andersen ◽  
Erik Ilsø Christensen ◽  
Hogne Vik

The tissue culture of multicellular spheroids from the renal epithelial cell line LLC-PK1 (proximal tubule) is described. This represents a biological system of intermediate complexity between renal tissue in vivo and simple monolayer cultures. The multicellular structures, which show many similarities to kidney tubules in vivo, including a vectorial water transport, should prove useful for studying the potential nephrotoxicity of drugs and chemicals in vitro. In addition, the propagation of renal epithelial cells as multicellular spheroids in serum-free culture may provide information on the release of specific biological parameters, which may be suppressed or masked in serum-supplemented media.


2020 ◽  
Vol 4 (3) ◽  
Author(s):  
C. Kons ◽  
Manh-Huong Phan ◽  
Hariharan Srikanth ◽  
D. A. Arena ◽  
Zohreh Nemati ◽  
...  

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