scholarly journals Effects of anesthetics pentobarbital sodium and chloral hydrate on urine proteome

2015 ◽  
Author(s):  
Mindi Zhao ◽  
Youhe Gao
Keyword(s):  
Nervous System ◽  
Chloral Hydrate ◽  
Biomarker Discovery ◽  
Animal Experiments ◽  
Label Free ◽  
Urinary Proteins ◽  
Healthy Human ◽  
Rat Urine ◽  
Pentobarbital Sodium ◽  
Urine Proteome

Background. Urine can be a better source than blood for biomarker discovery since it accumulates many changes. The urine proteome is susceptible to many factors including anesthesia. Pentobarbital sodium and chloral hydrate are commonly used anesthetics in animal experiments. Methods. This study demonstrated effects of these two anesthetics on the rat urine proteome using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Results. With anesthesia, the urinary protein-to-creatinine ratio of all rats increased two fold. The relative abundance of 22 and 23 urinary proteins were changed with pentobarbital sodium or chloral hydrate anesthesia, respectively, as determined by label-free quantification. Among these changed proteins, fifteen had been considered as candidate biomarkers such as uromodulin, sixteen had been considered stable in healthy human urine, which are more likely to be considered as potential biomarkers when changed, such as transferrin. Discussion. The pattern of changed urinary proteins provides clues to the discovery of urinary proteins regulatory mechanisms. When determining candidate biomarker, anesthetic-related effects can be excluded in future biomarker discovery studies. Since anesthetics take effects via nervous system, this study is the first to provide clues that protein handling function of kidney may possibly be regulated by nervous system.

scholarly journals Effects of anesthetics pentobarbital sodium and chloral hydrate on urine proteome

2015 ◽  
Author(s):  
Mindi Zhao ◽  
Youhe Gao
Keyword(s):  
Nervous System ◽  
Chloral Hydrate ◽  
Biomarker Discovery ◽  
Animal Experiments ◽  
Label Free ◽  
Urinary Proteins ◽  
Healthy Human ◽  
Rat Urine ◽  
Pentobarbital Sodium ◽  
Urine Proteome

Background. Urine can be a better source than blood for biomarker discovery since it accumulates many changes. The urine proteome is susceptible to many factors including anesthesia. Pentobarbital sodium and chloral hydrate are commonly used anesthetics in animal experiments. Methods. This study demonstrated effects of these two anesthetics on the rat urine proteome using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Results. With anesthesia, the urinary protein-to-creatinine ratio of all rats increased two fold. The relative abundance of 22 and 23 urinary proteins were changed with pentobarbital sodium or chloral hydrate anesthesia, respectively, as determined by label-free quantification. Among these changed proteins, fifteen had been considered as candidate biomarkers such as uromodulin, sixteen had been considered stable in healthy human urine, which are more likely to be considered as potential biomarkers when changed, such as transferrin. Discussion. The pattern of changed urinary proteins provides clues to the discovery of urinary proteins regulatory mechanisms. When determining candidate biomarker, anesthetic-related effects can be excluded in future biomarker discovery studies. Since anesthetics take effects via nervous system, this study is the first to provide clues that protein handling function of kidney may possibly be regulated by nervous system.

scholarly journals Effects of the glucocorticoid drug prednisone on urinary proteome and candidate biomarkers

2017 ◽  
Author(s):  
Jianqiang Wu ◽  
Xundou Li ◽  
Manxia An ◽  
Youhe Gao
Keyword(s):  
Drug Efficacy ◽  
Clinical Proteomics ◽  
Label Free ◽  
Urinary Proteins ◽  
Rat Urine ◽  
Urine Proteome ◽  
Disease Biomarkers ◽  
Urinary Proteome ◽  
Prednisone Treatment ◽  
Human Orthologs

AbstractUrine is a good source of biomarkers for clinical proteomics studies. However, one challenge in the use of urine biomarkers is that outside factors can affect the urine proteome. Prednisone is a commonly prescribed glucocorticoid used to treat various diseases in the clinic. To evaluate the possible impact of glucocorticoid drugs on the urine proteome, specifically disease biomarkers, this study investigated the effects of prednisone on the rat urine proteome. Urine samples were collected from control rats and prednisone-treated rats after drug administration. The urinary proteome was analyzed using liquid chromatography–tandem mass spectrometry (LC-MS/MS), and proteins were identified using label-free proteome quantification. Differentially expressed proteins and their human orthologs were analyzed with bioinformatics methods. A total of 523 urinary proteins were identified in rat urine. Using label-free quantification, 27 urinary proteins showed expression changes after prednisone treatment. A total of 16 proteins and/or their human orthologs have been previously annotated as disease biomarkers. After functional analysis, we found that the pharmacological effects of prednisone were reflected in the urine proteome. Thus, urinary proteomics has the potential to be a powerful drug efficacy monitoring tool in the clinic. Meanwhile, alteration of the urine proteome due to prednisone treatment should be considered in future disease biomarker studies.

scholarly journals Effects of anesthetics pentobarbital sodium and chloral hydrate on urine proteome

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e813 ◽  
Author(s):  
Mindi Zhao ◽  
Xundou Li ◽  
Menglin Li ◽  
Youhe Gao
Keyword(s):  
Chloral Hydrate ◽  
Pentobarbital Sodium ◽  
Urine Proteome

scholarly journals Proteomics in Diagnosis of Prostate Cancer/ Протеомика Во Дијагноза На Простатниот Карцином

PRILOZI ◽  
2015 ◽  
Vol 36 (1) ◽  
pp. 5-36 ◽  
Author(s):  
Katarina Davalieva ◽  
Momir Polenakovic
Keyword(s):  
Prostate Cancer ◽  
Tumor Tissue ◽  
Biomarker Discovery ◽  
Molecular Level ◽  
Specific Antigen ◽  
Shotgun Proteomics ◽  
Comparative Proteomics ◽  
Label Free ◽  
The Past ◽  
Proteomics Research

Abstract Prostate cancer (PCa) is the second most frequently diagnosed malignancy in men worldwide. The introduction of prostate specific antigen (PSA) has greatly increased the number of men diagnosed with PCa but at the same time, as a result of the low specificity, led to overdiagnosis, resulting to unnecessary biopsies and high medical cost treatments. The primary goal in PCa research today is to find a biomarker or biomarker set for clear and effecttive diagnosis of PCa as well as for distinction between aggressive and indolent cancers. Different proteomic technologies such as 2-D PAGE, 2-D DIGE, MALDI MS profiling, shotgun proteomics with label-based (ICAT, iTRAQ) and label-free (SWATH) quantification, MudPIT, CE-MS have been applied to the study of PCa in the past 15 years. Various biological samples, including tumor tissue, serum, plasma, urine, seminal plasma, prostatic secretions and prostatic-derived exosomes were analyzed with the aim of identifying diagnostic and prognostic biomarkers and developing a deeper understanding of the disease at the molecular level. This review is focused on the overall analysis of expression proteomics studies in the PCa field investigating all types of human samples in the search for diagnostics biomarkers. Emphasis is given on proteomics platforms used in biomarker discovery and characterization, explored sources for PCa biomarkers, proposed candidate biomarkers by comparative proteomics studies and the possible future clinical application of those candidate biomarkers in PCa screening and diagnosis. In addition, we review the specificity of the putative markers and existing challenges in the proteomics research of PCa.

NUTRITION, GROWTH AND NEUROINTEGRATIVE DEVELOPMENT: AN EXPERIMENTAL AND ECOLOGIC STUDY

PEDIATRICS ◽  
1966 ◽  
Vol 38 (2) ◽  
pp. 319-320 ◽  
Author(s):  
Joaquin Cravioto ◽  
Elsa R. DeLicardie ◽  
Herbert G. Birch
Keyword(s):  
Nervous System ◽  
Animal Experiments ◽  
Ecologic Study ◽  
Large Pool ◽  
Environmental Responsiveness ◽  
Social Custom ◽  
The Central Nervous System ◽  
Physical Stature ◽  
Inadequate Food Intake ◽  
Normal Controls

IN THIS MONOGRAPH we report the results of an experimental and ecologic study concerned with estimating some of the effects which malnutrition in early childhood may have upon neurointegrative functioning. In particular, we have been concerned with the association between malnutrition early in childhood and intersensory organization in children during the school years. A study of these relationships has derived from a concern with the possibility that inadequate food intake, particularly as represented by protein-calorie malnutrition, affects not only stature and weight, but also the capacity to learn. If this is indeed the case, then the significance of the observable and dramatic consequences of malnutrition for physical stature may be but one visible sign of functionally, perhaps, far more important non-visible handicapping. Findings on the effects which malnutrition has on the central nervous system suggest strongly that protein deficiency may result in structural lesions of the nervous system. Animals experimentally deprived are persistently delayed in achieving simple developmental landmarks, appear to be less adequate in environmental responsiveness and slower in learning as well as poorer in the retention of that which has been learned than normal controls. Considering the animal experiments and the findings in humans as a unit, one is led to be concerned with what in an ecologic sense could be called a "spiral" effect. A low level of adaptive capacity, ignorance, social custom, infection, or environmental paucity of foodstuffs appear to result in malnutrition which may produce a large pool of individuals who come to function in suboptimal ways.

Motor responses of Rhodnius prolixus Stål (Hem., Reduviidae) to volatile drugs

1971 ◽  
Vol 61 (2) ◽  
pp. 309-313 ◽  
Author(s):  
Ian McLure
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Carbon Tetrachloride ◽  
Chloral Hydrate ◽  
Rhodnius Prolixus ◽  
The Other ◽  
Motor Responses ◽  
Normal Feeding ◽  
Insect Central Nervous System ◽  
Differential Action

Fifth-instar nymphs of Rhodnius prolixus Stålwere exposed to the vapours of 11 volatile drugs: acetone, bromobenzene, bromoform, carbon tetrachloride, chloral hydrate, chloroform, dioxane, ethanol, ethyl ether, isopropanol and paraldehyde. Bromobenzene, bromoform, carbon tetrachloride, chloroform and ether induced reversible anaesthesia. For each of these five, the insects exhibited a different andspecific pattern of motor responses before becoming totally immobile; these responses are described. The responses to carbon tetrachloride are similar to the normal feeding responses of this insect. The other six drugs did not induce anaesthesia, but instead, a commonand stereotyped pattern of cleaning responses, suggesting irritation of the sensory organs. It is proposed that the agent-specific responses to the anaesthesiainducing drugs are due to their differential action upon specific portions of the insect central nervous system.

Sign in / Sign up

Export Citation Format

Share Document