scholarly journals Sensitivity distribution of Venturia inaequalis to fenarimol in Québec apple orchards

2005 ◽  
Vol 75 (1) ◽  
pp. 35-43 ◽  
Author(s):  
O. Carisse ◽  
J.R. Pelletier
Keyword(s):  
Growth Inhibition ◽  
Frequency Distribution ◽  
Radial Growth ◽  
Venturia Inaequalis ◽  
Apple Scab ◽  
Inhibition Assay ◽  
Baseline Sensitivity ◽  
Growth Inhibition Assay ◽  
Sensitivity Distribution ◽  
Ergosterol Synthesis

This study was initiated to quantify the baseline sensitivity of apple scab (Venturia inaequalis) to fenarimol, an ergosterol synthesis-inhibiting fungicide. In 1988, 576 monoconidial isolates of Venturia inaequalis were collected from 26 commercial orchards throughout Quebec. Sensitivity to fenarimol was assessed by radial growth inhibition assay. The ED50 values for the 26 orchards ranged from 0.024 to 5.212 (μ g mL-1 with a mean ED50 of 0.156 μg ml-1. Reduced sensitivity, expressed as ED50, was found in three orchards for an overall frequency of 4.51% of isolates. Sensitive isolates had a mean ED50 of 0.079 μg ml-1, whereas isolates with reduced sensitivity had a mean ED50 of 1.714 μ g mL-1, yielding a resistance factor of about 22. Four populations were identified based on the frequency distribution of ED50 values.

scholarly journals Baseline Sensitivity of Fusarium virguliforme to Fluopyram Fungicide

Plant Disease ◽  
2017 ◽  
Vol 101 (4) ◽  
pp. 576-582 ◽  
Author(s):  
Jie Wang ◽  
Carl A. Bradley ◽  
Olivia Stenzel ◽  
Dianne K. Pedersen ◽  
Ursula Reuter-Carlson ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Mycelial Growth ◽  
Soybean Seed ◽  
Inhibition Assay ◽  
Baseline Sensitivity ◽  
Growth Inhibition Assay ◽  
Fusarium Virguliforme ◽  
Fungicide Sensitivity ◽  
Conidia Germination

Fluopyram, a succinate dehydrogenase inhibitor (SDHI) fungicide, was recently registered for use as a soybean seed treatment for management of sudden death syndrome (SDS) caused by Fusarium virguliforme. Although registered and now used commercially, in vitro baseline fungicide sensitivity of F. virguliforme to fluopyram has not yet been established. In this study, the baseline sensitivity of F. virguliforme to fluopyram was determined using in vitro growth of mycelium and germination of conidia assays with two collections of F. virguliforme isolates. A total of 130 and 75 F. virguliforme isolates were tested using the mycelial growth and conidia germination assays, respectively, including a core set of isolates that were tested with both assays. In the mycelial growth inhibition assay, 113 out of 130 isolates (86.9%) were inhibited 50% by effective concentrations (EC50) less than 5 µg/ml with a mean EC50 of 3.35 µg/ml. For the conidia germination assay, 73 out of 75 isolates (97%) were determined to have an estimated EC50 of less than 5 µg/ml with a mean EC50 value of 2.28 µg/ml. In a subset of 20 common isolates that were phenotyped with both assays, conidia germination of F. virguliforme was determined to be more sensitive to fluopyram (mean EC50 = 2.28 µg/ml) than mycelial growth (mean EC50 = 3.35 µg/ml). Hormetic effects were observed in the mycelial growth inhibition assay as 22% of the isolates demonstrated more growth on medium amended with the lowest fluopyram concentration (1 µg/ml), as compared with the nonfluopyram amended control. It was not possible to determine EC50 values for nine out of 185 isolates (4.8%), as those isolates were not inhibited by 50% even at the highest fluopyram concentrations of 100 µg/ml for mycelial growth and 20 µg/ml for conidia germination inhibition assays. On the whole, the F. virguliforme population appears to be sensitive to fluopyram, and this study enables future monitoring of fungicide sensitivity.

A new diatom growth inhibition assay using the XTT colorimetric method

2016 ◽  
Vol 185-186 ◽  
pp. 13-19 ◽  
Author(s):  
Weina Jiang ◽  
Takuya Akagi ◽  
Hidekazu Suzuki ◽  
Ayaka Takimoto ◽  
Hiroshi Nagai
Keyword(s):  
Growth Inhibition ◽  
Colorimetric Method ◽  
Inhibition Assay ◽  
Growth Inhibition Assay

scholarly journals Application of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population

PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0184563 ◽  
Author(s):  
Richard Baguma ◽  
Adam Penn-Nicholson ◽  
Erica Smit ◽  
Mzwandile Erasmus ◽  
Jonathan Day ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Growth Inhibition ◽  
Whole Blood ◽  
Inhibition Assay ◽  
Growth Inhibition Assay ◽  
Mycobacterial Growth

scholarly journals Evaluation of Tuberculosis Vaccine Candidate, pcDNA3.1-rpfD using Mycobacterial Growth Inhibition Assay (MGIA)

2021 ◽  
Vol 29 (1) ◽  
pp. 1-8
Author(s):  
Mifa Nurfadilah ◽  
Andriansjah Rukmana ◽  
Fithriyah Sjatha
Keyword(s):  
Growth Inhibition ◽  
Vaccine Candidate ◽  
Negative Control ◽  
Automated System ◽  
Control Group ◽  
Functional Assay ◽  
Inhibition Assay ◽  
Peripheral Blood Mononuclear ◽  
Growth Inhibition Assay ◽  
Mycobacterial Growth

Resuscitation-promoting factor D (RpfD) is a protein involved in the resuscitation of dormant bacteria. A new tuberculosis vaccine carrying the rpfD gene has been successfully constructed, pcDNA3.1-rpfD. It was demonstrated that this vaccine exhibits cellular and humoral immune responses. Therefore, within this study, the efficacy of this new vaccine candidate was evaluated using mycobacterial growth inhibition assay (MGIA). MGIA is a functional assay that measures the complex host immune response, peripheral blood mononuclear cell (PBMC) and splenocyte from BALB/c mice against mycobacteria. With BACTECTM MGITTM 960 automated system, the effect of vaccination on bacterial growth was reported as a time to positivity (TTP) in hours. The mean of TTP from the vaccinated group (both pcDNA3.1-rpfD and BCG) was higher than the negative control group. These results suggest that pcDNA3.1-rpfD may be effective in controlling tuberculosis growth and may provide a clue for the development of the tuberculosis vaccine. In addition, despite previous evidence that IFNγ was essential for tuberculosis immunity, IFNγ (interferon gamma) production was found not to be correlated with mycobacterial inhibition. Therefore, these findings offer an alternative method to evaluate vaccine candidates than the assessment using IFNγ only.

scholarly journals Ex vivo mycobacterial growth inhibition assay (MGIA) for tuberculosis vaccine testing - a protocol for mouse splenocytes

2015 ◽  
Author(s):  
Andrea Andrea Zelmer ◽  
Rachel Tanner ◽  
Elena Stylianou ◽  
Sheldon Morris ◽  
Angelo Izzo ◽  
...  
Keyword(s):  
Immune Response ◽  
Clinical Trials ◽  
Growth Inhibition ◽  
Ex Vivo ◽  
Inhibition Assay ◽  
Growth Inhibition Assay ◽  
Mouse Splenocytes ◽  
Vaccine Testing ◽  
Mycobacterial Growth ◽  
Detailed Protocol

The testing of vaccines for tuberculosis is costly and time-consuming, and dependent on preclinical animal challenge models and clinical trials. We have recently developed a mycobacterial growth inhibition assay (MGIA) to test vaccine efficacy ex vivo. This assay measures the summative effect of the host immune response and may serve as a novel tool to facilitate vaccine testing. It has generated much interest recently, and to facilitate technology transfer and reproducibility between laboratories, we here describe a detailed protocol for an ex vivo MGIA in mouse splenocytes.

Evaluation of a Microbiological Growth Inhibition Assay as a Screening Test for the Presence of Antibiotic Residues in Poultry Meat

2007 ◽  
Vol 2 (5) ◽  
pp. 457-461 ◽  
Author(s):  
Muhammad Akbar Shahid ◽  
Muhammad Siddique ◽  
Sajjad-Ur-Rehman . ◽  
Sajid Hameed ◽  
Abid Hussain
Keyword(s):  
Growth Inhibition ◽  
Screening Test ◽  
Poultry Meat ◽  
Antibiotic Residues ◽  
Inhibition Assay ◽  
Growth Inhibition Assay
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