Evaluation of a Microbiological Growth Inhibition Assay as a Screening Test for the Presence of Antibiotic Residues in Poultry Meat

2007 ◽  
Vol 2 (5) ◽  
pp. 457-461 ◽  
Author(s):  
Muhammad Akbar Shahid ◽  
Muhammad Siddique ◽  
Sajjad-Ur-Rehman . ◽  
Sajid Hameed ◽  
Abid Hussain
Keyword(s):  
Growth Inhibition ◽  
Screening Test ◽  
Poultry Meat ◽  
Antibiotic Residues ◽  
Inhibition Assay ◽  
Growth Inhibition Assay

scholarly journals Sensitivity distribution of Venturia inaequalis to fenarimol in Québec apple orchards

2005 ◽  
Vol 75 (1) ◽  
pp. 35-43 ◽  
Author(s):  
O. Carisse ◽  
J.R. Pelletier
Keyword(s):  
Growth Inhibition ◽  
Frequency Distribution ◽  
Radial Growth ◽  
Venturia Inaequalis ◽  
Apple Scab ◽  
Inhibition Assay ◽  
Baseline Sensitivity ◽  
Growth Inhibition Assay ◽  
Sensitivity Distribution ◽  
Ergosterol Synthesis

This study was initiated to quantify the baseline sensitivity of apple scab (Venturia inaequalis) to fenarimol, an ergosterol synthesis-inhibiting fungicide. In 1988, 576 monoconidial isolates of Venturia inaequalis were collected from 26 commercial orchards throughout Quebec. Sensitivity to fenarimol was assessed by radial growth inhibition assay. The ED50 values for the 26 orchards ranged from 0.024 to 5.212 (μ g mL-1 with a mean ED50 of 0.156 μg ml-1. Reduced sensitivity, expressed as ED50, was found in three orchards for an overall frequency of 4.51% of isolates. Sensitive isolates had a mean ED50 of 0.079 μg ml-1, whereas isolates with reduced sensitivity had a mean ED50 of 1.714 μ g mL-1, yielding a resistance factor of about 22. Four populations were identified based on the frequency distribution of ED50 values.

A new diatom growth inhibition assay using the XTT colorimetric method

2016 ◽  
Vol 185-186 ◽  
pp. 13-19 ◽  
Author(s):  
Weina Jiang ◽  
Takuya Akagi ◽  
Hidekazu Suzuki ◽  
Ayaka Takimoto ◽  
Hiroshi Nagai
Keyword(s):  
Growth Inhibition ◽  
Colorimetric Method ◽  
Inhibition Assay ◽  
Growth Inhibition Assay

scholarly journals Application of a whole blood mycobacterial growth inhibition assay to study immunity against Mycobacterium tuberculosis in a high tuberculosis burden population

PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0184563 ◽  
Author(s):  
Richard Baguma ◽  
Adam Penn-Nicholson ◽  
Erica Smit ◽  
Mzwandile Erasmus ◽  
Jonathan Day ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Growth Inhibition ◽  
Whole Blood ◽  
Inhibition Assay ◽  
Growth Inhibition Assay ◽  
Mycobacterial Growth

scholarly journals Evaluation of Tuberculosis Vaccine Candidate, pcDNA3.1-rpfD using Mycobacterial Growth Inhibition Assay (MGIA)

2021 ◽  
Vol 29 (1) ◽  
pp. 1-8
Author(s):  
Mifa Nurfadilah ◽  
Andriansjah Rukmana ◽  
Fithriyah Sjatha
Keyword(s):  
Growth Inhibition ◽  
Vaccine Candidate ◽  
Negative Control ◽  
Automated System ◽  
Control Group ◽  
Functional Assay ◽  
Inhibition Assay ◽  
Peripheral Blood Mononuclear ◽  
Growth Inhibition Assay ◽  
Mycobacterial Growth

Resuscitation-promoting factor D (RpfD) is a protein involved in the resuscitation of dormant bacteria. A new tuberculosis vaccine carrying the rpfD gene has been successfully constructed, pcDNA3.1-rpfD. It was demonstrated that this vaccine exhibits cellular and humoral immune responses. Therefore, within this study, the efficacy of this new vaccine candidate was evaluated using mycobacterial growth inhibition assay (MGIA). MGIA is a functional assay that measures the complex host immune response, peripheral blood mononuclear cell (PBMC) and splenocyte from BALB/c mice against mycobacteria. With BACTECTM MGITTM 960 automated system, the effect of vaccination on bacterial growth was reported as a time to positivity (TTP) in hours. The mean of TTP from the vaccinated group (both pcDNA3.1-rpfD and BCG) was higher than the negative control group. These results suggest that pcDNA3.1-rpfD may be effective in controlling tuberculosis growth and may provide a clue for the development of the tuberculosis vaccine. In addition, despite previous evidence that IFNγ was essential for tuberculosis immunity, IFNγ (interferon gamma) production was found not to be correlated with mycobacterial inhibition. Therefore, these findings offer an alternative method to evaluate vaccine candidates than the assessment using IFNγ only.

scholarly journals Ex vivo mycobacterial growth inhibition assay (MGIA) for tuberculosis vaccine testing - a protocol for mouse splenocytes

2015 ◽  
Author(s):  
Andrea Andrea Zelmer ◽  
Rachel Tanner ◽  
Elena Stylianou ◽  
Sheldon Morris ◽  
Angelo Izzo ◽  
...  
Keyword(s):  
Immune Response ◽  
Clinical Trials ◽  
Growth Inhibition ◽  
Ex Vivo ◽  
Inhibition Assay ◽  
Growth Inhibition Assay ◽  
Mouse Splenocytes ◽  
Vaccine Testing ◽  
Mycobacterial Growth ◽  
Detailed Protocol

The testing of vaccines for tuberculosis is costly and time-consuming, and dependent on preclinical animal challenge models and clinical trials. We have recently developed a mycobacterial growth inhibition assay (MGIA) to test vaccine efficacy ex vivo. This assay measures the summative effect of the host immune response and may serve as a novel tool to facilitate vaccine testing. It has generated much interest recently, and to facilitate technology transfer and reproducibility between laboratories, we here describe a detailed protocol for an ex vivo MGIA in mouse splenocytes.

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