scholarly journals Transcriptome Assembly and Expression Data from Normal and Mantled Oil Palm Fruit

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Jeremy R. Shearman ◽  
Chatchawan Jantasuriyarat ◽  
Duangjai Sangsrakru ◽  
Thippawan Yoocha ◽  
Apichart Vannavichit ◽  
...  

We performed RNA sequencing of fruit from three normal and three mantled (somaclonal variant affecting flower development) oil palm plants using a 454 pyrosequencer. The three normal fruit samples were combined and sequenced, generating 237 748 reads. The three mantled fruit samples were combined and sequenced giving 231 438 reads. The reads were assembled into 13 984 sequences that were clustered into 10 218 genes or gene families. This paper describes the generation of this transcriptome database and includes annotation of these genes from Blast2GO and blast results against the Arabidopsis protein database as well as identification of putative transcription factors. In addition to this, the expression values for each gene sequence of the normal samples are presented. This dataset will be of use to anyone working in oil palm genetics.

2018 ◽  
Vol 24 (2) ◽  
pp. 67-76
Author(s):  
Sujadi Sujadi ◽  
Hasrul Abdi Hasibuan ◽  
Meta Rivani ◽  
Abdul Razak Purba

Fresh fruit bunches (FFB) consist of fruit be composed grade in few spikelet. Fruit at a spikelet can be distinguished into performed fruit namely internal fruit, middle fruit and outer fruit as soon as each section contain parthenocarpy fruits. This research was conducted for determine composition and content fatty acid of oil at internal fruit, middle, outer and parthenocarpy fruit from oil palm fruit. Samples of fruit came from 3 – 5 spikelet the central of FFB. Result showed that oil content of outer fruit (46.9 + 9.9)% trend higher be compared middle fruit (42.8 + 10.3)% and internal fruit (39.1 + 9.5)%. Parthenocarpy fruits have a low oil content (14.2 + 16.2)% except yellowish fruit trend high relatively oil content. The main components of fatty acid at outer fruit, middle and internal are palmitic acid, oleic, linoleic and stearic with mean value respectively (44.8 – 45.8)%, (37.6 – 38.0)%, (9.9 – 10.9)% and (4.6 – 4.8)%. Oil content at parthenocarpy fruit have amount main component of fatty acid with performed fruit but composition of palmitic acid (40.0 + 5.9)% and oleic (34.6 + 8.4)% lower while linoleic acid (16.9 + 8.5)% and linolenic (1.6 + 1.8)% higher be compared to performed fruit. Simalungun variety has the highest oil content in the part of fruit, with that PPKS 540 and La Mé respectively. PPKS 540 variety has the highest oleic acid content while PPKS 718 has the highest linoleic content.


2016 ◽  
Vol 24 (2) ◽  
pp. 67-76
Author(s):  
Sujadi Sujadi ◽  
Hasrul Abdi Hasibuan ◽  
Meta Rivani ◽  
Abdul Razak Purba

Fresh fruit bunches (FFB) consist of fruit be composed grade in few spikelet. Fruit at a spikelet can be distinguished into performed fruit namely internal fruit, middle fruit and outer fruit as soon as each section contain parthenocarpy fruits. This research was conducted for determine composition and content fatty acid of oil at internal fruit, middle, outer and parthenocarpy fruit from oil palm fruit. Samples of fruit came from 3 – 5 spikelet the central of FFB. Result showed that oil content of outer fruit (46.9 + 9.9)% trend higher be compared middle fruit (42.8 + 10.3)% and internal fruit (39.1 + 9.5)%. Parthenocarpy fruits have a low oil content (14.2 + 16.2)% except yellowish fruit trend high relatively oil content. The main components of fatty acid at outer fruit, middle and internal are palmitic acid, oleic, linoleic and stearic with mean value respectively (44.8 – 45.8)%, (37.6 – 38.0)%, (9.9 – 10.9)% and (4.6 – 4.8)%. Oil content at parthenocarpy fruit have amount main component of fatty acid with performed fruit but composition of palmitic acid (40.0 + 5.9)% and oleic (34.6 + 8.4)% lower while linoleic acid (16.9 + 8.5)% and linolenic (1.6 + 1.8)% higher be compared to performed fruit. Simalungun variety has the highest oil content in the part of fruit, with that PPKS 540 and La Mé respectively. PPKS 540 variety has the highest oleic acid content while PPKS 718 has the highest linoleic content.


2016 ◽  
Vol 24 (1) ◽  
pp. 47-55
Author(s):  
Agus Eko Prasetyo ◽  
Agus Susanto

Elaeidobius kamerunicus is the main pollinating agent in oil palm plantations in Indonesia today. The development of oil palm plantations in new areas requires introduction of these insects, moreover the new areas are located on different islands. First introduction of Elaeidobius kamerunicus Faust had been done from North Sumatra to Seram Island on 23 September 2013. The introduction was performed on larva and pupa stadium in 4-5 days post anthesising of male inflorescences. The introduction of E. kamerunicus using plywood boxes has an average risk of death by 7.89% at 6 days delivery period. Observation before releasing of the weevils showed that this insect was not detected in both of oil palm male and female flowers and the oil palm fruit set was very low approximately 11.27%. For about 500 weevils/ha were released in Marnuhu estate and could be developed into 362,401 weevils/ha in 5 months with 97.86% of oil palm sex ratio. The oil palm fruit sets were increased after 1 and 2 months introduction, 53.70% and 75.56 %, respectively. The lower sex ratio of oil palm or the more availability of male inflorescences make growth of E. kamerunicus population became faster and the greater number of weevils that visiting anthesising of female flowers make the higher value of oil palm fruit set.


2018 ◽  
Vol 24 (2) ◽  
pp. 77-86
Author(s):  
Sri Wening ◽  
Agus Eko Prasetyo ◽  
Tjut Ahmad Perdana Rozziansha ◽  
Agus Susanto

African pollination weevil (Elaeidobius kamerunicus Faust) has an important role in the productivity of Indonesian oil palm plantation. Up to now, there has not been a comprehensive biological study of the species at molecular level. The basic knowledge is very useful for exploitation of the weevil for effective oil palm fruit set development. This research aimed to obtain DNA extraction protocol of E. kamerunicus for DNA fingerprinting of the species. Results showed that using a DNA extraction kit,material disruption by using micro pestle resulted the highest quantity of DNA, while there were no significant differences of resulted DNA quantity among treatments using tissue lyser for material disruption. DNA extracted by using micro pestle or tissue lyser for material disruption is adequate for DNA fingerprinting using AFLP (Amplified Fragment Length Polymorphism) and sequencing techniques.


2018 ◽  
Vol 24 (1) ◽  
pp. 47-55
Author(s):  
Agus Eko Prasetyo ◽  
Agus Susanto

Elaeidobius kamerunicus is the main pollinating agent in oil palm plantations in Indonesia today. The development of oil palm plantations in new areas requires introduction of these insects, moreover the new areas are located on different islands. First introduction of Elaeidobius kamerunicus Faust had been done from North Sumatra to Seram Island on 23 September 2013. The introduction was performed on larva and pupa stadium in 4-5 days post anthesising of male inflorescences. The introduction of E. kamerunicus using plywood boxes has an average risk of death by 7.89% at 6 days delivery period. Observation before releasing of the weevils showed that this insect was not detected in both of oil palm male and female flowers and the oil palm fruit set was very low approximately 11.27%. For about 500 weevils/ha were released in Marnuhu estate and could be developed into 362,401 weevils/ha in 5 months with 97.86% of oil palm sex ratio. The oil palm fruit sets were increased after 1 and 2 months introduction, 53.70% and 75.56 %, respectively. The lower sex ratio of oil palm or the more availability of male inflorescences make growth of E. kamerunicus population became faster and the greater number of weevils that visiting anthesising of female flowers make the higher value of oil palm fruit set.


Author(s):  
Abdul Hallis Abdul Aziz ◽  
Siti Azura Abuzar ◽  
Mas Elyna Azol ◽  
Nurol Husna Che Rose ◽  
Rohaslida Liyana Mohmad

Genetics ◽  
1996 ◽  
Vol 144 (1) ◽  
pp. 329-338 ◽  
Author(s):  
R C Shoemaker ◽  
K Polzin ◽  
J Labate ◽  
J Specht ◽  
E C Brummer ◽  
...  

Abstract Restriction fragment length polymorphism mapping data from nine populations (Glycine max × G. soja and G. max × G. max) of the Glycine subgenus soja genome led to the identification of many duplicated segments of the genome. Linkage groups contained up to 33 markers that were duplicated on other linkage groups. The size of homoeologous regions ranged from 1.5 to 106.4 cM, with an average size of 45.3 cM. We observed segments in the soybean genome that were present in as many as six copies with an average of 2.55 duplications per segment. The presence of nested duplications suggests that at least one of the original genomes may have undergone an additional round of tetraploidization. Tetraploidization, along with large internal duplications, accounts for the highly duplicated nature of the genome of the subgenus. Quantitative trait loci for seed protein and oil showed correspondence across homoeologous regions, suggesting that the genes or gene families contributing to seed composition have retained similar functions throughout the evolution of the chromosomes.


2015 ◽  
Vol 802 ◽  
pp. 225-230
Author(s):  
Farah Noor Abdul Aziz ◽  
Sani Mohammed Bida ◽  
Noor Azline Mohd Nasir ◽  
Nor Azizi Safiee ◽  
Mohd Saleh Jaafar

Addition or replacement of waste tyre in mortars and concretes in lightweight aggregate concrete composites are popular in concrete material research although the mechanical properties of the composite are reduced. Various research studies have been conducted in an effort to improve the mechanical properties of concretes and mortars containing waste tyre particles using chemicals and additives which lead to increase cost. This approach presents an economical and sustainable method, through adding oil palm fruit fibre (OPFF) at 0.5, 1%, and 1.5% by mass of cement content into the matrix and pre-treating the tyre crumb aggregate (0-40%) by volume with cement, in order to improve the properties of the composite. Mechanical properties including compressive strength, split tensile strength and flexural strength were measured on the mortar specimens. Results showed the addition of 0.5% OPFF in 10% treated tyre crumb mortar gives the best improvement in the mechanical strengths of mortar modified with treated tyre crumb.


2010 ◽  
Vol 101 (2) ◽  
pp. 745-751 ◽  
Author(s):  
Hossein Mazaheri ◽  
Keat Teong Lee ◽  
Subhash Bhatia ◽  
Abdul Rahman Mohamed

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