scholarly journals Nanoscale microenvironment engineering based on layer-by-layer self-assembly to regulate hair follicle stem cell fate for regenerative medicine

Theranostics ◽  
2020 ◽  
Vol 10 (25) ◽  
pp. 11673-11689
Author(s):  
Peng Chen ◽  
Yong Miao ◽  
Feifei Zhang ◽  
Junfei Huang ◽  
Yuxin Chen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Regenerative Medicine ◽  
Hair Follicle ◽  
Cell Fate ◽  
Self Assembly ◽  
Layer By Layer ◽  
Stem Cell Fate ◽  
Hair Follicle Stem Cell

scholarly journals Polyamine-controlled proliferation and protein biosynthesis are independent determinants of hair follicle stem cell fate

2020 ◽  
Author(s):  
Kira Allmeroth ◽  
Christine S. Kim ◽  
Andrea Annibal ◽  
Andromachi Pouikli ◽  
Carlos Andrés Chacón-Martínez ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hair Follicle ◽  
Cell Fate ◽  
Protein Biosynthesis ◽  
Mrna Translation ◽  
Stem Cell Differentiation ◽  
List Type ◽  
Stem Cell Fate ◽  
Cell Fate Decisions ◽  
Hair Follicle Stem Cell

AbstractStem cell differentiation is accompanied by an increase in mRNA translation. The rate of protein biosynthesis is influenced by the polyamines putrescine, spermidine, and spermine that are essential for cell growth and stem cell maintenance. However, the role of polyamines as endogenous effectors of stem cell fate and whether they act through translational control remains obscure. Here, we investigated the function of polyamines in stem cell fate decisions using hair follicle stem cell (HFSC) organoids. HFSCs showed lower translation rates than progenitor cells, and a forced suppression of translation by direct targeting of the ribosome or through specific depletion of natural polyamines elevated stemness. In addition, we identified N1-acetylspermidine as a novel parallel regulator of cell fate decisions, increasing proliferation without reducing translation. Overall, this study delineates the diverse routes of polyamine metabolism-mediated regulation of stem cell fate decisions.Key PointsLow mRNA translation rates characterize hair follicle stem cell (HFSC) stateDepletion of natural polyamines enriches HFSCs via reduced translationN1-acetylspermidine promotes HFSC state without reducing translationN1-acetylspermidine expands the stem cell pool through elevated proliferation

scholarly journals N1-acetylspermidine is a determinant of hair follicle stem cell fate

2021 ◽  
Vol 134 (9) ◽  
Author(s):  
Kira Allmeroth ◽  
Christine S. Kim ◽  
Andrea Annibal ◽  
Andromachi Pouikli ◽  
Janis Koester ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hair Follicle ◽  
Cell Fate ◽  
Translational Control ◽  
Mrna Translation ◽  
Stem Cell Differentiation ◽  
Stem Cell Fate ◽  
Cell Fate Decisions ◽  
Hair Follicle Stem Cell

ABSTRACT Stem cell differentiation is accompanied by increased mRNA translation. The rate of protein biosynthesis is influenced by the polyamines putrescine, spermidine and spermine, which are essential for cell growth and stem cell maintenance. However, the role of polyamines as endogenous effectors of stem cell fate and whether they act through translational control remains obscure. Here, we investigate the function of polyamines in stem cell fate decisions using hair follicle stem cell (HFSC) organoids. Compared to progenitor cells, HFSCs showed lower translation rates, correlating with reduced polyamine levels. Surprisingly, overall polyamine depletion decreased translation but did not affect cell fate. In contrast, specific depletion of natural polyamines mediated by spermidine/spermine N1-acetyltransferase (SSAT; also known as SAT1) activation did not reduce translation but enhanced stemness. These results suggest a translation-independent role of polyamines in cell fate regulation. Indeed, we identified N1-acetylspermidine as a determinant of cell fate that acted through increasing self-renewal, and observed elevated N1-acetylspermidine levels upon depilation-mediated HFSC proliferation and differentiation in vivo. Overall, this study delineates the diverse routes of polyamine metabolism-mediated regulation of stem cell fate decisions. This article has an associated First Person interview with the first author of the paper.

scholarly journals First person – Kira Allmeroth

2021 ◽  
Vol 134 (9) ◽  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Fate ◽  
Early Career ◽  
First Person ◽  
Max Planck ◽  
Stem Cell Fate ◽  
Early Career Researchers ◽  
Hair Follicle Stem Cell ◽  
Selection Of

ABSTRACT First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Kira Allmeroth is first author on ‘ N1-acetylspermidine is a determinant of hair follicle stem cell fate’, published in JCS. Kira conducted the research described in this article while a PhD student in Martin Denzel's lab at the Max Planck Insitute for Biology of Ageing, Cologne, Germany. She is now a postdoc in the Denzel lab, investigating stem cells and metabolism.

scholarly journals Polyelectrolyte multilayers of poly (l-lysine) and hyaluronic acid on nanostructured surfaces affect stem cell response

Nanoscale ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 2878-2891 ◽  
Author(s):  
Marcus S. Niepel ◽  
Bhavya K. Ekambaram ◽  
Christian E. H. Schmelzer ◽  
Thomas Groth
Keyword(s):  
Stem Cell ◽  
Hyaluronic Acid ◽  
Cell Fate ◽  
Interference Lithography ◽  
Layer By Layer ◽  
Laser Interference Lithography ◽  
Laser Interference ◽  
Stem Cell Fate ◽  
Nanostructured Surfaces ◽  
First Time

Laser interference lithography (LIL) and layer-by-layer (LbL) technique are combined for the first time to design exceptional systems for control of stem cell fate.

Mechanoregulation of stem cell fate via micro-/nano-scale manipulation for regenerative medicine

Nanomedicine ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. 623-638 ◽  
Author(s):  
Chor Yong Tay ◽  
Cheng Gee Koh ◽  
Nguan Soon Tan ◽  
David Tai Leong ◽  
Lay Poh Tan
Keyword(s):  
Stem Cell ◽  
Regenerative Medicine ◽  
Cell Fate ◽  
Stem Cell Fate ◽  
Nano Scale

scholarly journals Glutamine Metabolism Controls Stem Cell Fate Reversibility and Long-Term Maintenance in the Hair Follicle

2020 ◽  
Vol 32 (4) ◽  
pp. 629-642.e8 ◽  
Author(s):  
Christine S. Kim ◽  
Xiaolei Ding ◽  
Kira Allmeroth ◽  
Leah C. Biggs ◽  
Olivia I. Kolenc ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hair Follicle ◽  
Cell Fate ◽  
Glutamine Metabolism ◽  
Stem Cell Fate ◽  
Term Maintenance

scholarly journals Orchestrating stem cell fate: Novel tools for regenerative medicine

2019 ◽  
Vol 11 (8) ◽  
pp. 464-475 ◽  
Author(s):  
Sara Cruciani ◽  
Sara Santaniello ◽  
Andrea Montella ◽  
Carlo Ventura ◽  
Margherita Maioli
Keyword(s):  
Stem Cell ◽  
Regenerative Medicine ◽  
Cell Fate ◽  
Stem Cell Fate
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