scholarly journals Noninvasive Imaging of Myocardial Inflammation in Myocarditis using 68Ga-tagged Mannosylated Human Serum Albumin Positron Emission Tomography

Theranostics ◽  
2017 ◽  
Vol 7 (2) ◽  
pp. 413-424 ◽  
Author(s):  
Seung-Pyo Lee ◽  
Hyung-Jun Im ◽  
Shinae Kang ◽  
Seock-Jin Chung ◽  
Ye Seul Cho ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Noninvasive Imaging ◽  
Emission Tomography ◽  
Myocardial Inflammation ◽  
Positron Emission

Noninvasive imaging of macrophages in rheumatoid synovitis using11C-(R)-PK11195 and positron emission tomography

2008 ◽  
Vol 58 (11) ◽  
pp. 3350-3355 ◽  
Author(s):  
Conny J. van der Laken ◽  
Ernst H. Elzinga ◽  
Mark A. Kropholler ◽  
Carla F. M. Molthoff ◽  
Joost W. van der Heijden ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Noninvasive Imaging ◽  
Emission Tomography ◽  
Positron Emission

scholarly journals Noninvasive Pulmonary [18F]-2-Fluoro-Deoxy-d-Glucose Positron Emission Tomography Correlates with Bactericidal Activity of Tuberculosis Drug Treatment

2009 ◽  
Vol 53 (11) ◽  
pp. 4879-4884 ◽  
Author(s):  
Stephanie L. Davis ◽  
Eric L. Nuermberger ◽  
Peter K. Um ◽  
Camille Vidal ◽  
Bruno Jedynak ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Bactericidal Activity ◽  
Fdg Pet ◽  
Point Of Care ◽  
Noninvasive Imaging ◽  
Emission Tomography ◽  
Response To Treatment ◽  
Tb Treatment ◽  
Positron Emission ◽  
Drug Regimens

ABSTRACT Tools for monitoring response to tuberculosis (TB) treatment are time-consuming and resource intensive. Noninvasive biomarkers have the potential to accelerate TB drug development, but to date, little progress has been made in utilizing imaging technologies. Therefore, in this study, we used noninvasive imaging to monitor response to TB treatment. BALB/c and C3HeB/FeJ mice were aerosol infected with Mycobacterium tuberculosis and administered bactericidal (standard and highly active) or bacteriostatic TB drug regimens. Serial pulmonary [18F]-2-fluoro-deoxy-d-glucose (FDG) positron emission tomography (PET) was compared with standard microbiologic methods to monitor the response to treatment. [18F]FDG-PET correctly identified the bactericidal activity of the drug regimens. Imaging required fewer animals; was available in real time, as opposed to having CFU counts 4 weeks later; and could also detect TB relapse in a time frame similar to that of the standard method. Lesion-specific [18F]FDG-PET activity also broadly correlated with TB treatment in C3HeB/FeJ mice that develop caseating lesions. These studies demonstrate the application of noninvasive imaging to monitor TB treatment response. By reducing animal numbers, these biomarkers will allow cost-effective studies of more expensive animal models of TB. Validated markers may also be useful as “point-of-care” methods to monitor TB treatment in humans.

Cerebral oxygen delivery by liposome-encapsulated hemoglobin: a positron-emission tomographic evaluation in a rat model of hemorrhagic shock

2007 ◽  
Vol 103 (1) ◽  
pp. 28-38 ◽  
Author(s):  
Vibhudutta Awasthi ◽  
Seong-Hwan Yee ◽  
Paul Jerabek ◽  
Beth Goins ◽  
William T. Phillips
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Rat Model ◽  
Oxygen Delivery ◽  
Oxygen Metabolism ◽  
Blood Substitutes ◽  
Oxygen Carriers ◽  
Positron Emission Tomographic ◽  
Positron Emission

Liposome-encapsulated Hb (LEH) is being developed as an artificially assembled, low-toxicity, and spatially isolated Hb-based oxygen carrier (HBOC). Standard methods of evaluating oxygen carriers are based on surrogate indicators of physiology in animal models of shock. Assessment of actual delivery of oxygen by HBOCs and resultant improvement in oxygen metabolism at the tissue level has been a technical challenge. In this work, we report our findings from 15O-positron emission tomographic (15O-PET) evaluation of LEH in a rat model of 40% hypovolemic shock. In vitro studies showed that PEGylated LEH formulation containing ∼7.5% Hb and consisting of neutral lipids (distearoylphosphatidylcholine:cholesterol:α-tocopherol, 51.4:46.4:2.2) efficiently picks up 15O-labeled oxygen gas. The final preparation of LEH contained 5% human serum albumin to provide oncotic pressure. Cerebral PET images of anesthetized rats inhaling 15O-labeled O2 gas showed efficient oxygen-carrying and delivery capacity of LEH formulation. From the PET images, we determined cerebral metabolic rate of oxygen (CMRO2) as a direct indicator of oxygen-carrying capacity of LEH as well as oxygen delivery and metabolism in rat brain. Compared with control fluids [saline and 5% human serum albumin (HSA)], LEH significantly improved CMR[Formula: see text] to ∼80% of baseline level. Saline and HSA resuscitation could not improve hypovolemia-induced decrease in CMR[Formula: see text]. On the other hand, resuscitation of shed blood was the most efficient in restoring oxygen metabolism. The results suggest that 15O-PET technology can be successfully employed to evaluate potential oxygen carriers and blood substitutes and that LEH resuscitation in hemorrhage enhances oxygen delivery to the cerebral tissue and improves oxygen metabolism in brain.

scholarly journals Microfluidic Preparation of 89Zr-Radiolabelled Proteins by Flow Photochemistry

Molecules ◽  
2021 ◽  
Vol 26 (3) ◽  
pp. 764
Author(s):  
Daniel F. Earley ◽  
Amaury Guillou ◽  
Dion van der Born ◽  
Alex J. Poot ◽  
Jason P. Holland
Keyword(s):  
Positron Emission Tomography ◽  
Human Serum Albumin ◽  
Radiochemical Purity ◽  
Flow Reactor ◽  
Radiochemical Yield ◽  
Emission Tomography ◽  
Flow Process ◽  
Automated Production ◽  
Desferrioxamine B ◽  
Positron Emission

89Zr-radiolabelled proteins functionalised with desferrioxamine B are a cornerstone of diagnostic positron emission tomography. In the clinical setting, 89Zr-labelled proteins are produced manually. Here, we explore the potential of using a microfluidic photochemical flow reactor to prepare 89Zr-radiolabelled proteins. The light-induced functionalisation and 89Zr-radiolabelling of human serum albumin ([89Zr]ZrDFO-PEG3-Et-azepin-HSA) was achieved by flow photochemistry with a decay-corrected radiochemical yield (RCY) of 31.2 ± 1.3% (n = 3) and radiochemical purity >90%. In comparison, a manual batch photoreactor synthesis produced the same radiotracer in a decay-corrected RCY of 59.6 ± 3.6% (n = 3) with an equivalent RCP > 90%. The results indicate that photoradiolabelling in flow is a feasible platform for the automated production of protein-based 89Zr-radiotracers, but further refinement of the apparatus and optimisation of the method are required before the flow process is competitive with manual reactions.

scholarly journals Noninvasive imaging of islet grafts using positron-emission tomography

2006 ◽  
Vol 103 (30) ◽  
pp. 11294-11299 ◽  
Author(s):  
Y. Lu ◽  
H. Dang ◽  
B. Middleton ◽  
Z. Zhang ◽  
L. Washburn ◽  
...  
Keyword(s):  
Positron Emission Tomography ◽  
Noninvasive Imaging ◽  
Emission Tomography ◽  
Positron Emission
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